磷酸酶

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

The optimal pH was 8.7. Immunofluorescent and AP-immunocytochemical assays revealed that the HO distributed dispersively in the worm, and located in cytoplasm.

成虫组织切片显示，特异性荧光在虫体内呈散在性分布，细胞碱性磷酸酶免疫染色试验表明该酶位于成虫的细胞质。

Consequently, the enzymatic hydrolyzable P may contribute to the internal loading of the eutrophic lake, and stratification of kinetics and compositions of phosphatase in shallow lake is theoretically noticeable.

因此，富营养湖泊沉积物中磷的&内负荷&应有可酶解有机磷的含义，浅水湖泊中磷酸酶的活性组成和动力学参数的分层现象当为湖沼学研究中值得深入探讨的新理论问题。

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

After cultured for 4 days,the cells were stained with TRAP. Multinucleated(≥ 3 nuclei) TRAP-positive cells were counted as osteoclasts, and the expression of TRAP mRNA were determined by RT-PCR.

2.0？10.0？50.0 ？滋g/ml，对获得的各组破骨细胞行抗酒石酸酸性磷酸酶染色计数阳性破骨细胞(核≥ 3)的数目；提取总RNA用逆转录-聚合酶链反应检测TRAP mRNA的表达？

Results:There were no abdominal pain and fever,jaundice faded away gradually postoperation.Biliary drainage was 300ml-1000ml/d more or less.Colour of bile changed into normal by degrees.Intra peritoneal drainage was open and the colour was light bloody.Amount of drainage dropped off day after day.Whole blood cell test, serum glutamic pyruvic transaminase,serum glutamic oxalacetic transarninase,total serum protein,serum albumin and serum globulin returned to normal in first week postoperation.Serum bilirubin,serum glutamyltranspetidase and serum alkaline phosphatase declined gradually in first week postoperation.T-tube was clipped at twelfth day postoperation.T-tube Cholangiography was clear at 24~ day postoperation.

结果：术后无上腹部绞痛、高热，黄疸逐渐减退，每日引流胆汁300ml-1000ml不等，由淡黄色混浊、内有胆泥过渡到金黄色清亮的正常胆汁，腹腔引流通畅，引流液为淡红色，量从最初的200ml迅速过渡到10ml，血常规、血清谷丙转氨酶、谷草转氨酶、总蛋白、白蛋白、球蛋白等1周内均恢复正常，血清总胆红素、直接胆红素、间接胆红素、谷氨酰转肽酶、碱性磷酸酶逐渐下降，术后12d夹闭T管，术后24d T管造影提示左、右肝管及胆总管下端通畅，无狭窄。

Pharmacodynamics experiments indicated that the drug obviously degraded the content of Cholesterol,Triglycerides,glutamic pyruvic transaminase and Maleic Dialdehyde in the Alcoholic fatty liver of rats and mice treated with by oral administration, it also degraded the content of glutamic oxalacetic transaminase, r-GLU,alkaline phosphatase and increased the content of Albumin and Superoxide Dismutase in the Alcoholic fatty liver of rats, and remarkably reduced depressant effect of the mice'centra nerve.

药效学实验表明，经预防灌胃给药，可以显著降低酒精性脂肪肝大鼠和小鼠，总胆固醇、甘油三酯、谷丙转氨酶、丙二醛含量，降低酒精性脂肪肝大鼠谷草转氨酶、γ-谷氨酰基转移酶、碱性磷酸酶含量，增加白蛋白和超氧化物歧化酶含量。

Pinctada fucata ; Alkaline phosphatase; Enzyme activity

合浦珠母贝；碱性磷酸酶；酶活性

Kummerva M Localization of acid phosphatase in maize root under phosphorus deficieacy Biologia Plantarium , 1986,null

李玉京%李滨低磷营养胁迫条件下小麦RNA酶与酸性磷酸酶活性的变化西北植物学报， 1997，4

One pt at the 30,000-mg/day dose level experienced reversable, asymptomatic grade III elevation of gamma glutamyl transferase and grade II elevation of alkaline phosphatase during a second cycle.

一个病人在第二个疗程用到每天3万毫克时出现了可逆性的3级咖玛gultamyl转移酶升高，和2级碱性磷酸酶升高。但没有临床症状。三个病人治疗两个疗程后停止了治疗。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。