磷酸酶

Methods: MTT and enzyme dynamics methods were used to observe the effects of CAP on cell proliferation and alkaline phosphatase activity of HDFC.

分别采用MTT比色法和酶动力学方法观察牛牙骨质附着蛋白对体外培养人牙囊细胞增殖及其碱性磷酸酶活性影响。

ALP is an enzyme found in high concentrations in the liver and bile ducts, as well as some other tissues.

碱性磷酸酶是一种酶中发现高浓度的肝和胆管，以及其他一些组织。

This paper deals with the changes in ATPase and PPase activities, lipid compositon of tonoplast vesicles from two wheat cultivars, Altas 66(Al-tolerant) and Scout 66(Al-sensitive) under Al stress.

刘友良 ，沈振国，何龙飞研究了铝胁迫下耐铝性不同的两个小麦品种根细胞液泡膜ATP酶、焦磷酸酶活性和膜脂的变化。

Methods:A rabbit model was developed with scald complicated with artificial infection of Pseudomonas aeruginosa.Studies were carried out by using the combat trauma spray group and the solution group.At day1,2,4,7,14,post experiments,rabbit serum was collected,the body temperatures of animals were taken,and such inflammatory factors as white cell count,serum levels of NO 2-/NO 3-,serum levels of tumor necrosis factor,serum superoxide anion,as well as GPT,AKP,aspartate carbamyl transferase,urea nitrogen were also collected to measure the functions of both the kidney and liver.

采用烫伤合并人工感染绿脓杆菌的家兔模型，用战伤喷剂与溶剂组于实验前和实验后第1、2、4、7、14天采集家兔血清，测量体温，检测白细胞数、血清NO 2-/NO 3-水平、血清肿瘤坏死因子水平、血清超氧阴离子等炎性因子水平以及谷丙转氨酶、碱性磷酸酶、天冬氨酸转移酶、肌苷和尿素氮等肝肾功能指标水平。

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞，使之转化为成骨细胞；用RT-PCR检验成骨细胞；不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基，作用于成骨细胞，并分析生长曲线，分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织，用Percoll分离液分离出骨髓中的单个核细胞，在含体积分数为10%小牛血清的高糖DMEM培养液中，置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养，通过传代培养扩增骨髓基质干细胞，传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养，用倒置显微镜、HE染色观察增殖和分化情况，并测定碱性磷酸酶活性和钙结节形成能力。

The effect of the concentration, pH of several ligands such as acetate, oxalate, tartrate and phosphate solution and their orders added on the adsorption and activity of BSA and acid phosphatase on yellow brown soil, latosol, kaolinite, goethite and -MnO2 was first systematically studied by using batch and FTIR methods after analyzed and studied the relative literature that reported in the world.

论文在分析总结了国内外有关研究的基础上，用批量法和傅立叶变换红外光谱法首次系统地研究了乙酸、草酸、酒石酸和磷酸几种配体的浓度、pH及加入顺序对牛血清白蛋白、酸性磷酸酶在黄棕壤、砖红壤、高岭石、针铁矿和二氧化锰表面的吸附行为及活性的影响，探讨了这些配体对蛋白吸附机理及二级结构的影响。

The effect of the concentration, pH of several ligands such as acetate, oxalate, tartrate and phosphate solution and their orders added on the adsorption and activity of BSA and acid phosphatase on yellow brown soil, latosol, kaolinite, goethite and δ-MnO〓 was first systematically studied by using batch and FTIR methods after analyzed and studied the relative literature that reported in the world. The impact of these ligands on the adsorption mechanism and secondary structure of protein was simultaneously engaged.

论文在分析总结了国内外有关研究的基础上，用批量法和傅立叶变换红外光谱法首次系统地研究了乙酸、草酸、酒石酸和磷酸几种配体的浓度、pH及加入顺序对牛血清白蛋白、酸性磷酸酶在黄棕壤、砖红壤、高岭石、针铁矿和二氧化锰表面的吸附行为及活性的影响，探讨了这些配体对蛋白吸附机理及二级结构的影响。

Duque G, Rivas D. Alendronate has an anabolic effect on bone through the differentiation of mesenchymal stem cells.

与这些研究结果类似，笔者在实验中发现阿仑磷酸钠和辛伐他汀都能明显刺激成骨细胞的增殖，并且能显著刺激成骨细胞分泌碱性磷酸酶，表明两种药物对骨形成都有一定的促进作用。

The classic osteoblast-induced system including 0.1μmol/L dexamethasone, 10 mmol/Lβ-glycerophosphate, 50μg/mL Vitamin C was used to induce osteoblastic differentiation of BMSCs. Through the detection of alizarin red staining and quantitative, the ALP activity and the expression of OCN-mRNA, it explored the effect of osteoblastic differentiation of BMSCs on Bushen Therapy, Huatan Therapy, Bushen Huatan Therapy.

使用0.1μmol/L地塞米松，10 mmol/Lβ-甘油磷酸钠，50μg/mL Vitamin C联合诱导BMSCs成骨分化，通过检测茜素红染色及定量、碱性磷酸酶活性和骨钙素mRNA表达，探讨补肾中药、化痰中药和补肾化痰中药对BMSCs成骨分化的影响。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。