磷酸酶

Result Activity of serum alkalinity phosphatase of cows and sheep was in a normal range. But activity of alkalinity phosphatase of mid pregnancy cows was obviously lower than before pregnancy and not pregnant and later pregnancy (P.05). Alkaline phosphatase retention was lower than 26%. It showed that serum alkalinity phosphatase of cows and sheep came from the skeleton.

结果］所有奶牛和绵羊血清碱性磷酸酶活性均在正常范围内，但奶牛怀孕中期的碱性磷酸酶活性比未怀孕、怀孕前期、怀孕后期明显降低(P.05)；碱性磷酸酶保存率均低于26%，表明奶牛和绵羊血清碱性磷酸酶均来自骨骼。

When PO3-4-P concentrations exceeded 0.010mg·L-1, the TAPA was significant difference (P＜0.01).②The contents of APA in phytoplankton, bacteria and dissolved were different. The ratio of DAPA to TAPA was the highest and BAPA was the smallest.

水体中的碱性磷酸酶在不同粒级组分中的分布差异明显，其中以溶解性碱性磷酸酶活性所占的比例最大，浮游植物体中的碱性磷酸酶活性所占的比例次之，细菌体中的碱性磷酸酶活性所占的比例最小。

The results indicated that AKP exist in all tissues and organs of Andrias davidianus, which were mainly 230 and 200 KD. Activity of AKP in different tissues and organs was different, and these were strongest in heart and weakest in eyes. In the same tissues and organs, two kinds of AKP activities were different.

结果表明，二十四种组织器官中都有碱性磷酸酶存在，其中230和200 KD的碱性磷酸酶占优势；不同组织器官的碱性磷酸酶活性有差异，心脏中活性最强，眼球中活性最弱；在同一组织中，230和200 KD的碱性磷酸酶活性也有差异。

That means Pho85 kinaseand calcineurin were involved in salt tolerance with an identical target protein or in 中科院上海生化与细胞所博士学位论文摘要 the same pathway. Inhibition of calcineurin decrease the YPH499, pho80? mutant,and pap1 (pcl7)? mutant Mn2+ tolerance but not that of pho85? mutant and thepho85? mutant was more sensitive to Mn2+ than YPH499, pho80? mutant, and pap1(pcl7)? mutant even with the addition of cyclosporin A. Therefore, the conclusioncould be drawn that PHO85 gene played a dominant role in Mn2+ homeostasisregulation in compare with calcineurin. As for Ca2+ tolerance, cyclosporin A canincrease the tolerance to Ca2+ of all the mutant mention above, that means Pho85kinase and calcineurin function antagonistically in regulation of Ca2+ homeostasis.In Bioinformatics, BRI3 gene is a novel gene without any function clue but givehigh conservation in mammalian.

我们通过钙调磷酸酶的特异抑制剂环孢菌素A研究了YPH499、+2+pho85缺失株、pho80缺失株、pap1缺失株在钙调磷酸酶失活后对Na、Mn、2+Ca金属离子敏感性的变化，结果显示Pho85蛋白激酶和钙调磷酸酶通过直接+或间接激活同一个靶蛋白或途径来增强细胞对Na的耐受；和钙调磷酸酶相比，2+PHO85的缺失对酵母细胞Mn耐受性的破坏是相对控制性的，钙调磷酸酶的失2+活不能进一步降低pho85缺失株对Mn的耐受能力；Pho85和钙调磷酸酶在对2+Ca的耐受调节中是相互拮抗的，钙调磷酸酶的失活能增加pho85缺失株和2+pho80缺失株的Ca耐受。i中科院上海生化与细胞所博士学位论文摘要对本实验室克隆的人新基因BRI3进行系统的生物信息学分析，发现它是一个在哺乳动物中保守的，但功能未知的基因。

The main results were as follows:There was significant correlation between the soil urease and phosphatase activities, which showed no significant difference upon different plots. The urease and phosphatase activities in the soil were higher in spring than autumn, which under mangrove plants were all higher than those in the New alluvial soil without plants and Bared land. Soil urease activity exhibited no significant difference under different mangrove species. The phosphatase activity in the soil of Centenary Aegiceras corniculatum and Spartina alterniflor was the highest, which was the higher in the soil of Kandelia candel and Avicennia marine.

结果表明：土壤脲酶和磷酸酶活性存在极显著的相关性；两种酶在不同区域间均无显著差异，季节变化均表现为春季大于秋季；有红树植被的土壤脲酶和磷酸酶活性皆大于空地和裸地，土壤脲酶不同红树植被下无显著差异，磷酸酶则表现为百年生桐花树和互花米草显著高于其余植被，秋茄和白骨壤次之。

The hepatic glycogen and the activities of ACP, LDH, returned to normal at 7th postoperative day in the Labiatae group.

3丹参组术后3天琥珀酸脱氢酶、镁离子激活三磷酸腺苷酶、葡萄糖-6-磷酸酶活性升高，术后7天肝糖原和乳酸脱氢酶、酸性磷酸酶活性回到正常水平。

Detect with Western Blot protein of MAb counterpoise form and the natural phosphoric acid is enzymatic reactivity in the cell, obtain 6 individual plant to be able to be stabilized in all secrete the individual plant of cross tumour cell that fights phosphoric acid enzymatic MAb, go enzymaticly to study double particularity phosphoric acid further phosphation action mechanism and its activation formerly in mitosis way of enzymatic signal of albumen phosphoric acid turns guide provide strong tool.

用Western blot检测mAb对重组蛋白和细胞中天然磷酸酶的反应性，共获得6株可稳定分泌抗磷酸酶mAb的杂交瘤细胞株，为进一步探究双特异性磷酸酶的去磷酸化功能机理以及其在有丝分裂原激活蛋白磷酸酶信号途径中的信号转导提供强有力的工具。

By TUNEL and ultrastructural observation,the results showed that cadmium toxication caused apoptosis in testes,and adding selenium may decrease the effect. 5.Cadmium can affect the activites of several enzymes in biomembrane, Such as SDH,ALP,Mg2+-ATPase,G-6-P,Ca2+-ATPase and lead to metabolical disorder in tissues.

通过酶组化检测，证明镉能影响公鸡睾丸组织中酶活性，即琥珀酸脱氢酶、碱性磷酸酶、Mg~(2+)激活的三磷酸腺苷酶Mg~(2+-ATPase、葡萄糖-6磷酸酶(G-6-Pase)、Ca~(2-)激活的三磷酸腺苷酶Ca~(2--ATPase活性下降，酸性磷酸酶活性升高，导致睾丸组织损伤。

Both of the venoms showed activities of multiplehydrolases including hyaluronidase, protease, DNase, acid phosphetase,alkaline phosphetase and choline esterase, of which hyaluronidase, protease and DNase were more active. The activity of protease in VDPGwas higher than that of protease in VES and the activities of the resthydrolases in VDPG were lower than those of hydrolases in VES.

通过对粗毒进行酶活性分析，发现两种粗毒都具有透明质酸酶、蛋白水解酶、DNA酶、酸性磷酸酶、碱性磷酸酶、乙酰胆碱酯酶等酶活性；其中透明质酸酶、蛋白水解酶、DNA酶活性较高；毒囊粗毒中蛋白水解酶活性高于电刺激粗毒，而其它几种酶活性均低于电刺激粗毒。

Protein phosphatases are crucial for cell living. Protein phosphatase 2A (PP2A) is an important member of serine/theronine protein phosphatase family, which is involved in almost all biological processes in eukaryotic cell. The 3D structure of PP2A core enzyme and holoenzyme was solved in 2006. These results are significant instructions for us to further understand PP2A structure, interactions between PP2A subunits, and also interactions between PP2A and its binding proteins.

蛋白磷酸酶在细胞的生命活动中起着十分重要的作用，蛋白磷酸酶2A(protein phosphatase 2A， PP2A)作为蛋白磷酸酶家族中十分重要的一员，它几乎与所有真核细胞的生命活动都有密不可分的关系。2006年，PP2A核心酶和全酶晶体结构的陆续破解对于深入了解PP2A自身的结构和亚基之间的相互作用，以及其与结合蛋白作用的机制都有重大的影响。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。