磷酸酰胺
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Amino-6-methoxy Benzothiazole 99%、2-Amino Benzothiazole 99%、Veratraldehyde 99%、Veratric Acid 99%、1,2- Dimethoxy Benzene 99%、6-Nitroveratric Acid 97%、2,5-Dichlorobenzoic Acid 99%、4-Chloro-3-Nitrobenzoic Acid 99%、 3-Nitrobenzole Acid 98%、p-Methoxybenzoie Acid 99%、Anisole 99.5%、p-Methoxyacetophenonc 99%、(2'-Chloroethyl) Benzene 99%、(2'-Bromoethyl) Benzene 99%、N,N-Diethyi-m-Toiuamide 99%、Aminoacetonitrile Hydrochloride 98%、 Alkyl Diphenyl Phosphate Estrel 98%、5-Chloro-2-benzothiazolone 99%、6-bromo-2-naphthol 98%、6-Nitro-Veretric Acid Estrel 97%、4-chloro phenoxyaeetic 99
2-氨基-6-甲氧基苯骈噻唑、2-氨基苯骈噻唑、藜芦醛、藜芦酸、藜芦醚、6-硝基藜芦酸、2,5-二氯苯甲酸、3-硝基-4-氯苯甲酸、3-硝基苯甲酸、对甲氧基苯甲酸、苯甲醚、对甲氧基苯乙酮、β-氯代苯乙烷、β-溴代苯乙烷、N,N-二乙基间甲苯甲酰胺、氨基乙腈盐酸盐、磷酸二苯月桂酯、5-氯-2-苯骈噻唑酮、6-溴-2-萘酚、6-硝基藜芦酸甲酯、4-氯苯氧乙酸
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In this paper, the study status of enhancing the protein emulsification by modification methods such as deamidation, proteolysis, glycosylation, phosphorylation and graft copolymerization were introduced, the development direction of the preparation of new type protein emulsifier was also pointed out,it provided a reference for the future study.
综述了脱酰胺作用、蛋白水解作用、糖基化作用、磷酸化作用、接枝改性等蛋白质改性技术对提升蛋白质乳化性的研究现状,展望了制备新型蛋白质乳化剂的发展方向,为今后蛋白质乳化剂的研究提供参考。
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To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.
为研究磷酸化应激诱导蛋白的表达、定位和功能,采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段,经产物纯化、与载体连接,分别构建了STIP1基因的原核表达载体和真核表达载体;IPTG诱导原核表达载体,变性聚丙酰胺凝胶电泳与染色后,可见相应大小的蛋白质表达;STIP1基因的真核表达载体转染细胞系后,共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内;用药物筛选STIP1基因转染的细胞后,流式细胞仪检测细胞周期,可见G〓-G〓期和G〓-M期的细胞明显降低,S期细胞明显增多。
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BMSCs were isolated from adult rats by modified bone marrow culture method. BMSCs were inoculated in basic medium containing 10%fetal bovine serum, high glucose DMEM, 100 U/L penicillin, 100 U/L streptomycin and 2 mmol/L glutamine in the control group. BMSCs were inoculated in conditioned medium (basic medium, 10 mmol/L β-glycerophosphoric sodium, 10-7 mol/L dexamethasone, 50 mg/L vitamin C).
通过改良的骨髓培养法分离成年大鼠骨髓间充质干细胞,对照组以基础培养液(10%胎牛血清+高糖DMEM+100U/L青霉素+100 U/L链霉素+2 mmol/L谷氨酰胺)培养,实验组用条件培养液(基础培养液,10 mmol/Lβ-甘油磷酸纳,10-7 mol/L地塞米松,50 mg/L维生素C)进行诱导培养。
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In order to make WRCRFs with good performance, the raw material fertilizers with smaller effects on HWAR such as urea, monoammonium phosphate and potassium chloride should be selected. On the other hand, for HWAR, high stability and adequate WAR such as polyacry lamide graft polymer should be needed.
制造性能优越的保水型控释肥,应尽可能选择对HWAR稳定性影响较小的尿素、磷酸一铵、氯化钾等原料肥料,对于包膜材料则应选择稳定性高且具适度吸水倍率的聚丙烯酰胺型HWAR。
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SOD, ATPase, MDH and ME isoenzymes in gill, digestive gland, foot, adductor muscle and mantle of Mytilus edulis were tested and analyzed using polyacrylamide gel electrophoresis.
采用聚丙烯酰胺垂直电泳法对紫贻贝 Mytilus edulis 的鳃、消化腺、外套膜、足、闭壳肌等5种组织的超氧化物歧化酶、三磷酸腺苷酶、苹果酸脱氢酶、苹果酸酶进行了检测分析。
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Thioamide was refluxed with sodium hypophosphite and ppt-Ni prepared beforehand from NiCl〓·6H〓O and Zn powder in a mixture of dioxane and water to give amines in 60-98% yields (12 examples).
催化剂Ni-Zn是用氯化镍和锌粉反应制成,为合金型固体粉末,次磷酸钠作为氢供体,对12种硫代酰胺进行脱硫还原,收率60-98%。
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Results Patients who had at least one abnormal result of liver function test accounted for 70.99% in untreated group and 61.07% in treated group. The most common abnormal hepatic function parameter in patients with thyrotoxic hepatic lesion was increased alkaline phosphate enzyme, which was 57.3% in untreated group and 51.0% in treated group. Abnormalities of alanine transaminase, aspartate transaminase, gamma-glutamyl tyanspeptidase and total bilirubin were 28.2%(37/131), 18.3%(24/131), 10.7%(14/131) and 21.4%(28/131) in untreated group and 21.5%(32/149), 15.4%(23/148), 4.7%(7/149) and 20.1%(30/149) in treated group, respectively. The possibility of thyrotoxic hepatic dysfunction was correlated with the patients' age and FT3 and FT4 levels, but not with the genesis and course of disease, sex, family history of hyperthyroidism, or levels of TGAb and TMAb. Conclusion Abnormal results of liver function test are commom in patients with hyperthyroidism.
结果 在未治组甲亢性肝功能损害的发生率为70.99%,经治组为61.07%;甲亢性肝功能损害最常见的异常指标是碱性磷酸酶增高,未治组、经治组分别为57.3%、51.0%;血清谷丙转氨酶异常在两组的发生率分别为28.2%、21.5%;血清谷草转氨酶异常在两组的发生率分别为18.3%、15.4%;总胆红素异常在两组的发生率分别为21.4%、20.1%;谷酰胺转肽酶异常的发生率未治组和经治组分别为10.7%、4.7%;甲亢性肝功能损害的发生与病程、甲状腺疾病家族史、性别、TGAb水平、TMAb水平无关,与年龄、甲状腺激素水平有关。
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Sulfuric acid series, hydrochloric acid (AR hydrochloride, CP hydrochloric acid) series, nitric acid, phosphoric acid, boric acid, protopine, soda ash, ammonia, acetic acid, bleaching powder, chloride, alkaline chlorine of aluminum, nickel chloride, nickel sulphate, polyacrylamide, such as hydrogen peroxide.
硫酸系列,盐酸系列,硝酸,磷酸,硼酸,片碱,纯碱,氨水,冰醋酸,漂白粉,聚合氯化铝,碱式氯化铝,氯化镍,硫酸镍,聚丙烯酰胺、双氧水等。
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The mobile phase was 0.1 mol/L ammonium oxalate + N,N-dimethylformamide + 0.2 mol/L diammonium phosphate (75+15+10, by vol) adjusted to pH 8 by aqueous ammonia. Column temperature was 30℃, flow rate was 0.8 ml/min, and detection wavelength was 365 nm.
使用C18反相柱和二极管阵列检测器,以0.1mol/L草酸铵溶液+二甲基甲酰胺+0.2mol/L磷酸氢二铵溶液=75+15+10(用氨水调pH值至8.0左右)为流动相,柱温30℃,流速0.8ml/min,检测波长365nm,外标法对水合霉素的有效成分进行定量分析。
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For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether
年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。
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Eph. 4:23 And that you be renewed in the spirit of your mind
弗四23 而在你们心思的灵里得以更新
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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.
老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。