磷酸酯合成酶

We have been interested in design and synthesis of macrocyclic lanthanide complexes which are capable of catalytically hydrolyzing phosphodiester bonds. Because of their high thermodynamics stability, low kinetics lability, high coordination numbers, and charge density, macrocyclic lanthanide complexes will be suitable as artificial nucleases.

合成设计能促进磷酸酯键水解的金属错合物，一直是我们实验室很感兴趣的研究领域，尤其是以镧系金属离子大环配位子错合物这方面的研究，因其具有高度的热力学稳定、动力学稳定、高配位数及电子密度等特性，使得镧系金属大环错合物非常适合作为人造水解酶。

In search of databases, the deduced products of sanP, sanQ, sanR, sanS, sanT and sanU show highest similarity to those of nikP2, nikQ, nikR, nikS, nikT and nikU of S. tendae respectively. In comparison with the proteins of identified function, it is indicated that sanP encodes a thioesterase, sanQ encodes a cytochrome P450, sanR encodes a uracil phosphoribosyltransferase, sanS encodes a carboxylase, sanT encodes a histidinol-phosphate aminotransferase and sanU encodes a mutase.

在蛋白数据库中比较结果表明，sanP、sanQ、sanR、sanS、sanT和sanU基因编码的蛋白分别和唐德链霉菌的尼可霉素生物合成基因nikP2、nikQ、nikR、nikS、nikT、nikU六个基因编码的蛋白同源性最高；根据和已知功能蛋白的比较，推测sanP基因编码的是硫酯酶，sanQ基因编码的是细胞色素P450，sanR基因编码的是尿嘧啶磷酸核糖转移酶，sanS基因编码的是羧化酶，sanT基因编码的是组氨醇磷酸氨基转移酶，sanU基因编码的是一种变位酶。

TPP gene plays an important role in synthesis of trehalose. The cloning of TPP gene lays a solid basis for investigating the biosynthesis of trehalose in L. bicolor.

海藻糖－6-磷酸磷酸酯酶在海藻糖合成过程中起关键作用，该基因的克隆为研究海藻糖在二色补血草中的合成奠定了基础。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

This product can suppress the activity of PDE III, reduce the infiltration of oil, and promote the synthesis of hormone-sensitive type esterase, thus has the effects of decomposing and releasing lipocyte lipid.

抑制磷酸二酯酶Ⅲ活性，减少油脂渗入，并促进激素敏感型酯酶的合成，从而具有分解和释放脂肪细胞内脂质的作用。

A series of pH- and temperature-sensitive hydrogels carrying pendant phosphategroups have been synthesized by copolymerizing 2-methacryloyloxyethyl dihydrogenphosphate with N-isopropylacrylamide. The swelling ratio, swelling and deswellingkinetics have been investigated. High loading percentage of lysozyme into hydrogelshas been achieved and the release profile has been studied.

通过N-异丙基丙烯酰胺和含磷酸基团的单体2-甲基丙烯酰乙氧基二氢磷酸酯的共聚合，合成了一类含磷酸基团的既对温度敏感又对pH敏感的水凝胶，研究了它们在不同pH及温度时的溶胀率及溶胀动力学，并初步研究了其对溶菌酶的释放行为。

Metal complex and metallomicelle as model compounds for artificial enzyme have been widely and extensively studied. In this dissertation, a series of homo- and hetero-dinuclear oxamido-bridged complexes have been synthesized, and the catalytic hydrolyses of carboxylic acid esters and phosphate by these complexes have been systematically investigated, and the catalytic mechanisms have also been dissected.

金属配合物及金属胶束作为人工酶的模型化合物受到广泛而深入的研究，本文通过合成一系列的草酰胺桥联同核和异核双核配合物，较为系统地研究了它们催化羧酸酯、磷酸酯水解反应的动力学，探讨了它们的作用机制；同时，本文还探索了将金属胶束这一较为成功的水解酶模拟模型应用于过氧化物酶模拟研究的可行性。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。