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We collected Leishmania promastigotes,washed its with 0.01mol/LpH7.2 buffers ,smashed its,and then measured protein content and titrated work concentration.2.We tested Kala-azar patients"serum and healthy persons"serum of epidemic area by ELISA. The results of three antigensmethods were the same, work concentration of serum was the same(l :100).Results:The protein content of three antigens is, 901 strain with 1000ug/ml,801 strain with 960ug/ml and 951 strain with 1100ug/ml. Suitable work concentration is, 901 strain with 10ug/ml ,801 strain with 12ug/ml and 951 strain with 11ug/ml.

1、抗原的提取:收集新疆不同地区分离得到的利什曼原虫前鞭毛体,经培养,用0.01mol/L pH7.2的磷酸盐缓冲液漂洗,超声粉碎,比色法测定其蛋白质含量,间接ELISA法滴定出抗原的工作稀释度。2、检测:用提取的三株抗原,分别进行间接ELISA法测定黑热病病人血清和疫区健康人血清抗体,并以肺结核病人血清、非疫区健康人血清做对照,三株抗原所用检测方法一致,血清工作稀释度均为1∶100。

Methods Streptomycin and dihydrostreptomycin were extracted from honey samples using a liquid extraction with a phosphate buffer followed by a solid-phase extraction based on weak cation-exchange columns. After sample preparation, the hydrophilic interaction chromatography was used to retain streptomycin and dihydrostreptomycin on the column for the separation of the analytes from endogenous compounds. The aminoglycosides were detected and quantified with electrospray ionization tandem mass spectrometry.

方法用磷酸盐缓冲溶液提取蜂蜜中的链霉素和双氢链霉素后,经弱阳离子交换柱进行固相萃取,样品制备后采用亲水作用色谱,使链霉素和双氢链霉素实现保留,从而与内源性物质达到分离,采用电喷雾离子化串联质谱法检测和定量。

A derivative of amphetamine, such as dextroamphetamine or a phosphate or sulfate of amphetamine, used as a central nervous system stimulant in the treatment of certain conditions, such as narcolepsy and depression.

硫酸苯丙胺安非他命的衍生物,如右旋安非他命,或其磷酸盐或硫酸盐,在治疗某种病状如发作性睡病和精神抑郁时可用作中枢神经系统兴奋剂

DNA is composed of a backbone made from deoxyribose and phosphate.

DNA是由脱氧核糖组成了脊梁和磷酸盐

In DNA the corresponding sugar is the closely related deoxyribose. A ribose molecule combined with adenine, guanine, cytosine, or uracil forms a nucleoside; adding a phosphate group forms a nucleotide.

去氧核糖核酸中相应的糖类和去氧核糖密切相关核糖分子和腺嘌呤、鸟嘌呤、胞嘧啶或尿嘧啶形成核;再加上磷酸盐和磷酸酯便成为核。

The chlortetracycline biosynthetic rate got to 140 u/h-mL and was more than that ofcontrol by 50% when 0.1% glutamine was added in fermentation broth in cell growth phase.

碳在HMS途径的通量对金霉素的合成起重要的调节作用,添加芳香性氨基酸〓和高磷酸盐抑制碳在HMS的通量,金霉素最终合成量降低50%以上。

Only the thick blob of blue-green algae will thrive in low phosphate environment , and so over time this species tends to dominate these stable systems .

在低磷酸盐的环境中,唯一能够繁荣兴盛的只有大块的蓝绿海藻,因此,随着时间的推移,这个物种势必控制这些稳定的系统。

Only the thick blob of blue-green algae will thrive in low phosphate environment , and so over time this species tends to dominate these stable systems .

在低磷酸盐的环境中,唯一能够繁荣兴盛的只有大块的蓝绿海藻,那么,随着时间的推移,这个物种势必在这些稳定系统中占据主位。

Only the thick blob of blue-green algae will thrive in low phosphate environment , and so over time this species tends to dominate these stable systems .

在低磷酸盐的环境中,唯一能够繁荣兴盛的只有大块的蓝绿海藻,因而,随着时间的推移,这个物种势必占据这些稳定的系统。

That process may have started inadvertently in 1956 when the British administration of the Gilbert Islands in Micronesia put a levy on the export of phosphates-bird manure-used in fertiliser.

这个过程在1956年本来可以自然开始的,那时在位于密克罗尼西亚的吉尔伯特群岛上英国管理部门对出口用作肥料的磷酸盐-鸟的粪肥-强制增税。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。