磷酸原

Phosphorylase mRNA wasless in proembryos, but significantly increased in differentiating embryos accumulatingtransition starch.

原胚中的磷酸化酶mRNA表达较弱，到分化胚积累过渡性淀粉时才显著增加。

College of Life Science, Wuhan University, Wuhan 430072On the basis of optimization of in situ RNA hybridization technique, Westudied the temporal and spatial expression of calmodulin and phosphorylasemRNA and its relation with fertilization and embryogenesis in rice as well as antherdevelopment in tabacco. We also constructed cDNA libraries of proembryos anddifferentiating young embryos in rice using microscopic isolation and RT-PCRtechniques.

本文在优化RNA原位杂交技术的基础上，研究了钙调素mRNA和磷酸化酶mRNA时空表达及其与水稻受精和胚胎发生以及烟草花药发育的关系；利用显微分离技术和RT-PCR技术建立了水稻原胚和分化幼胚cDNA文库。

Aliquots were subjected to impedance aggregometryin response to collagen, adenosine diphosphate, ristocetin,and arachidonic acid performed at 22°C, 34°C, 37°C,and 40°C.

一部分用作在22°C 、34°C 、37°C 和40°C 下测定对胶原、二磷酸腺苷、瑞斯西丁素和花生四烯酸反应中的阻抗集合度测试。

METHODS: The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition, and cultured in vitro with collagenase digestion. All cells were divided into 3 groups: in the BMP-2 group, cells were cultured with medium containing 0.1 g/L vitamin C, 10 mmol/Lβ-sodium glycerophosphate and 10μg/L BMP-2 for 10 minutes, followed by 4-14 days inoculation with density of 18×104 cells per pore. In the BMP-7 group, cells were cultured with BMP-7 with the same methods as BMP-2 group. The cells were cultured with simple culture medium in the control group.

无菌切取成年新西兰大白兔皮下脂肪，胶原酶消化法体外分离培养脂肪干细胞，分为3组：骨形成蛋白2组加入含0.1 g/L维生素C、10 mmol/L β-甘油磷酸钠、10 μg/L骨形成蛋白2的诱导培养基培育15 min，然后按18×104个细胞/孔接种，再培育4~14 d；骨形成蛋白7组培养方法基本相同，仅将骨形成蛋白2更换为骨形成蛋白7；对照组同法加入单纯培养基进行培育。

Methods Primary brown adipocytes from rat fetuses, and from wild-type neonate mice and neonate mice deficient in the gene encoding protein tyrosine phosphatase-1B (Ptpn1, also known as Ptp1b) were cultured in the absence or presence of TNFα and different nuclear receptor agonists.

原代棕色脂肪细胞自大鼠胚胎、野生型新生小鼠和蛋白酪氨酸磷酸酶-1B基因基因(Ptpn1，也被称作 Ptp1b)缺陷新生小鼠，在加或者不加TNFα和不同浓度核受体激动剂中培养。

Mol. Cell. Biol. 19:7491 7500.[2].Bassett,C.A.,Pawluk,R.J.,and Pilla,A.A.(1974)Augmentation of bone repair by inductively coupled electromagnetic fields. Science 184,575-577[3]. Scott, G., and King, J.

脉冲电磁场促进骨髓间充质干细胞碱性磷酸酶的表达以及成骨指标如 osteotrix ，骨型态蛋白4和 I 型胶原的表达。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。