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Four weeks after operation, a large quantity of rosy newly formed bone and green bone trabecula were showed in the BG-COL-PS-HYA group. At week 12, scaffold materials was substituted by new bone tissues basically, and Haversian system was visible. Eight weeks after operation, the results of X-ray film revealed that cortical bone was fully connected.

生物活性玻璃/胶原蛋白/透明质酸/磷酸丝氨酸组术后4周硬组织切片可见大量玫瑰红色新骨和绿色骨小梁形成,术后12周支架已基本由新生骨组织替代,哈弗系统形成;术后8周X射线显示骨皮质连接完整,12周缺损完全修复,髓腔基本再通。

The cells were identified by invert phase contrast microscopy and alkaline phosphatase staining and mineralized nodules staining.The activity distribution of osteoblasts was determined by microtitration during a culture cycle.

通过倒置相差显微镜观察,并用碱性磷酸酶和矿化结节染色鉴定,利用微量滴定法测定一个培养周期的成骨细胞活性分布,探讨一种经济、高效的原代成骨细胞培养方法。

AIM: To examine the role of matrix metalloproteinases expressed by the tartrate-resistant acid phosphatase-positive mononuclear and multinucleated cells in articular cartilage damage.

目的:观察在胶原诱导的C57BL/6小鼠类风湿性关节炎模型中,基质金属蛋白酶-2、9在耐酒石酸盐酸性磷酸酶阳性的单个核及多核细胞中的表达及其在关节软骨损伤中的作用。

Objective:To investigate the relationship of the expression of phosphorylation p38 mitogen activated protein kinase(p-p38MAPK) and the inflammatory damage of dorsal root ganglion and neuropathological pain caused by non-compressed nucleus pulposus in rats.

目的:观察背根神经节磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)表达的变化与非压迫性髓核所致的炎性反应及病理性神经痛的关系。

Sodium orthovanadate 1 mmol·L-1, a potent inhibitor of protein tyrosine phosphatases, blocked GST-induced inhibition of Ica,L.

酪氨酸磷酸酶抑制剂原钒酸钠可阻断GST对钙通道电流的抑制作用。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后,碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性;成脂诱导后油红染色呈阳性,有一定数量的脂滴形成。

Expression of collagen I,II and alkaline phos┐phatase in osteogenesis by rhBMP┐2in vivo

rhBMP┐2在体内诱导成骨中I,II型胶原及碱性磷酸酶的表达

Cells in the bron choalveolar lavage fluid were counted and differentiated, as well as the amounts of total protein, albumin and the activities of alkaline phos phatase, lactate dehydrogenase and type Ⅳ collagenase (matrix metallco proteinases, MMP-2, MMP-9) were detected.

计数支气管肺泡灌洗液中的细胞数并行分类,测定总蛋白、白蛋白含量及碱性磷酸酶、乳酸脱氢酶及Ⅳ型胶原酶(MMP-2、MMP-9)活性。

Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China AIM: To observe the in situ phosphated expression of p38 MAPK in lung tissues of acute lung injury rat model induced by Lipopolysaccharide.

目的:观察磷酸化的分裂原激活的蛋白激酶(p38 MAPK)在脂多糖引起的大鼠急性肺损伤中在体原位的表达。

Methods:The RP-HPLC method was applied and protocatechuic aldehyde was used as the internal standard substance.

采用RP-HPLC法,以原儿茶醛为内参照物,色谱柱:Luna C_(18)柱(250mm×4.6mm,5μm),流动相:0.05%磷酸水溶液-乙腈,梯度洗脱程序为:0min时A-B(100∶0),80min时A-B(67∶33),85min时A-B(60∶40),100min时A-B(60∶40),流速:1.0mL·min~(-1),检测波长:280nm。

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