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Experiments show that Ang Ⅱ activates JNK mediated by AT〓, and JNK increases transcription activity of c-Jun by phosphorylating c-Jun amido-terminal, and induces expression of c-Fos gene. c-Jun and c-Fos form transcriptional factor activated protein-1 complex. AP-1 may stimulates genes which correlate with cardiovascular remodeling expression, such as transforming growth factor, collagen type Ⅰ and Ⅲ, skeletal muscle α-actin,β-MHC, atrial natriuretic factor.

实验表明,在心肌和血管平滑肌细胞Ang Ⅱ通过AT〓介导激活JNK;JNK通过磷酸化c-Jun氨基末端增加c-Jun的转录活性并诱导c-Fos基因表达,c-Jun和c-Fos蛋白形成转录因子复合物激活蛋白-1(AP-1),并刺激各种心血管重塑相关基因如转化生长因子-β1(TGF-β1)、Ⅰ,Ⅲ型胶原、骨骼肌α-actin、β-MHC(β-myosin heavy chain,β-肌球蛋白重链)、心钠素的表达。

Objective To explore the apoptosis induced by dexamethasone and adenosine triphosphate in protoscolex of Echinococcus granulosus .

摘要目的探讨地塞米松与三磷酸腺苷联用在体外诱导细粒棘球绦虫原头节细胞凋亡的作用。

In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞,运用活细胞计数和碘化丙吮检测其增殖能力;采用流式细胞术检测其细胞表面标志的表达;用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化,并用Von Kossa染色进行鉴定;用地塞米松与胰岛素诱导其向脂肪细胞分化,并以油红O染色进行鉴定。

Based on the retrieval and review of the bibliographer, the original process for the preparation of Dimethyl Phosphite in Zhejiang Xinan Chemical co., Ltd was evaluated.

论文在文献阅译和工艺原理分析的基础上,结合浙江新安化工集团股份有限公司原亚磷酸二甲酯生产装置的实际,研究了以甲醇、三氯化磷为原料的无溶剂的连续化生产新工艺。

HGF can regulate the proliferation and differentiation of chondrocyte both in vivo and in vitro.

外源性HGF能加强骨、软骨的形态发生,提高培养软骨细胞Ⅱ型胶原mRNA的表达和碱性磷酸酶活性。

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞,使之转化为成骨细胞;用RT-PCR检验成骨细胞;不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基,作用于成骨细胞,并分析生长曲线,分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

Storage temperature of 22°C exclusively enhancedadenosine diphosphate- and collagen-induced aggregation comparedwith storage at 37°C.

存储温度22°C 相较37°C 而言是唯一可以增强二磷酸腺苷和胶原诱导的凝集反应。

Starch phosphate is one kind of starch derivative produced from esterification of starch and phosphate.

淀粉磷酸酯是原淀粉与磷酸盐发生酯化反应而生成的一种淀粉衍生物。

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞,DMEM、新生牛血清培养基进行原代培养,部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化,用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

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