磷酸原

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果：提取液主要为Ⅰ型胶原蛋白；72小时37℃恒温培养箱内培养无细菌生长；紫外分光光度计测定，吸收高峰为230nm；氨基酸成分主要为甘氨酸：占40%，脯氨酸：14.3%。显微镜下见：相互交织的胶原原纤维结构组成的网状结构；纤维均匀，有规则横纹。胶原蛋白液在37℃恒温下，可制成凝胶，用于培养细胞；用磷酸液冲洗胶原蛋白凝胶可成固体片状，制成胶原膜，用作医用材料。

Owing to phosphorous acid is produced from phosphorus trichloride in industry by hydrolysts, inspissation and crystallization, if we use phosphorus trichloride directly on synthesize N- iminodiacetic acid, not only can simplify operation, but also can red.

原工艺采用的是在酸性条件下高温时将甲醛滴加到亚磷酸与亚氨基二乙酸一钠盐混合溶液中进行反应，考虑到工业上亚磷酸的合成一般采用三氯化磷水解、提浓、结晶制得，直接将三氯化磷用于双甘膦的合成，不仅可以简化操作过程，而且对于降低生产成本和能耗有明显意义。

Originally techniques dropped formaldehyde into the mixture of phosphorous acid and monosodium of imino diacetic acid in high temperature. Owing to phosphorous acid is produced from phosphorus trichloride in industry by hydrolysis, inspissation and crystallization, if we use phosphoros trichloride directly on synthesize N- iminodiacetic acid, not only can simplify operation, but also can reduce the cost and save energy.

原工艺采用的是在酸性条件下高温时将甲醛滴加到亚磷酸与亚氨基二乙酸一钠盐混合溶液中进行反应，考虑到工业上亚磷酸的合成一般采用三氯化磷水解、提浓、结晶制得，直接将三氯化磷用于双甘膦的合成，不仅可以简化操作过程，而且对于降低生产成本和能耗有明显意义。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

Detect with Western Blot protein of MAb counterpoise form and the natural phosphoric acid is enzymatic reactivity in the cell, obtain 6 individual plant to be able to be stabilized in all secrete the individual plant of cross tumour cell that fights phosphoric acid enzymatic MAb, go enzymaticly to study double particularity phosphoric acid further phosphation action mechanism and its activation formerly in mitosis way of enzymatic signal of albumen phosphoric acid turns guide provide strong tool.

用Western blot检测mAb对重组蛋白和细胞中天然磷酸酶的反应性，共获得6株可稳定分泌抗磷酸酶mAb的杂交瘤细胞株，为进一步探究双特异性磷酸酶的去磷酸化功能机理以及其在有丝分裂原激活蛋白磷酸酶信号途径中的信号转导提供强有力的工具。

The psammoma body mineralization in meningioma is a common type of mineralizationThe analysis of the mineral composition may provide some support information in finding the reason of happening and developing of the diseaseThis paper focuses on the concentric layered structure mineralization in meningiomas, using mineralogical methods, such as HRSEM, ESEM, EDAX, EPMA, HRTEM, XRD and FTIR to systematically investigate the mineral composition, structure and shape of the minerals in psammoma bodies in meningiomasWe have devised a method for preparing the silicon wafer sheet which was used for the ESEM insitu observations and analysisIn this study, we first got the ESEM and HRTEM images of the initial mineralization phase of meningiomasThese images showed that in the early stage of psammoma body mineralization in meningiomas, many mineralized balls composed of octocaphosphate were precipitated on the collagen fibersThese balls continued to grow and aggregate, and were gradually hydrolyzed to become the dahlliteThe continued development of mineralization resulted in the mineralized collagen fibersThe study revealed that the concentric layered structure of the psammoma bodies in meningiomas is formed by the spiral arrangement of the mineralized collagen fibers on which the mineralized grains precipitated.

砂粒体矿化是脑膜瘤中常见的矿化类型，对其形成机理和矿物成分的分析可能会对肿瘤发生、发展的研究提供辅助信息。该研究选取人脑膜瘤中的砂粒体矿化作为研究对象，采用偏光显微镜、环境扫描电镜及能谱、X射线衍射仪、高分辨透射电镜和电子探针对样品的形貌、结构和成分进行测试分析，并以此为依据探讨脑膜瘤中砂粒体的形成机理。研究结果表明矿化的初期为沉淀在胶原纤维上的矿化小球，成分为磷酸八钙；矿化小球不断生长聚集，并逐步水解为碳羟磷灰石晶体，矿化的不断发展致使胶原纤维也发生矿化。砂粒体的同心层状构造是由螺旋状排列的矿化胶原纤维及沉淀在其上的矿化颗粒组成的集合体，而不是多数研究中所述：砂粒体是以坏死细胞残骸为中心由内至外的同心层沉淀。

The effects of corn starch, potato starch, sweet potato starch, and their modified forms, oxidized potato starch, acetified potato starch, phosphorylated potato starch on qualities and textures of sausage .

实验主要研究了玉米淀粉、马铃薯淀粉、红薯淀粉的3种不同的原淀粉以及氧化马铃薯淀粉、醋酸化马铃薯淀粉、磷酸化马铃薯淀粉三种改性淀粉对灌肠质构的影响，结果发现：在相同条件下，玉米原淀粉灌肠的硬度高于马铃薯和红薯原淀粉灌肠的硬度，但是在弹性、结着性上马铃薯淀粉灌肠更好。

Therefore, pretreatment of raw flax was performed with selected chelating agents such as ethylene diamine tetraacetic acid, sodium tripolyphosphate, oxalic acid and ammonium oxalate.

选用EDTA、多聚磷酸钠、草酸和草酸铵等螯合剂对亚麻原麻进行了预处理，结果表明：EDTA、草酸、多聚磷酸钠的处理效果均不理想，改变浓度、温度等条件，韧皮与麻秆并没有分离；而草酸铵是比较有效的螯合剂，用5 g/L的草酸铵在62℃处理亚麻原麻5 h后，韧皮与麻秆能够完全分离。

Class-specific polyclonal antibodies for O,O-diethyl phosphorothioate pesticides had been generated. A series of generic haptens were synthesized and coupled to bovine serum albumin and ovalbumin for immunogens and coating antigens, respectively. New Zealand rabbits were immunized with immunogens to obtain corresponding polyclonal antibodies.

通过分析硫代磷酸二乙酯类农药的结构特点，设计并合成了系列半抗原；采用活泼酯法将半抗原分别与牛血清蛋白和卵清蛋白偶联制备了系列免疫原和包被原；通过免疫新西兰大白兔获得了相应抗硫代磷酸二乙酯类农药的类特异性抗体。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。