磷酸化酶

Results At the 7th day after osteoblastic induction, ALP was strongly positive, and at the 10th day, mineralized nodules stained with chinalizarin were jacinth. At the 21th day after adipogenic induction, the adipocytes were stained with oil red O.

结果 BMSCs在成骨诱导液中7d 碱性磷酸酶呈强阳性，10d 矿化结节染色呈橘红色；在成脂诱导液中，21d 油红O染色呈阳性。

The following data demonstrated that ALP secretion of differentiated hMSC increased significantly compared with undifferentiated cells. And the matrix secreted by differentiated hMSC began to mineralize after 14 days.

结果 经过诱导分化的骨髓基质细胞，碱性磷酸酶分泌明显增加，可以产生大量的矿化结节，具有成熟成骨细胞的表型特征。

This paper reviewed its pathogenesis, forms, clinical manifestations, differential diagnosis, treatment and prognosis. Here a case of neonatal hypophosphatasia is reported. This baby was female (30 minutes old). Prenatal ultrasound showed disproportionate biparietal diameter and long bones of limbs in the baby. After birth, she presented with obvious craniomalacia, respiratory distress and cyanosis. Serum alkaline phosphatase level was significantly reduced. Both X-ray and autopsy showed extremely insufficient skeletal mineralization. Four days later she died of respiratory failure.

该文对其发病机制、分型及临床表现、鉴别诊断、治疗和预后进行了综述，并介绍了在该院诊断明确的1例新生儿型的罕见病例，患儿为出生30 min女婴，产前B超提示胎儿双顶径与四肢长骨不成比例，生后即有明显的颅骨软化、呼吸困难和紫绀等表现，血碱性磷酸酶显著低下，X线表现及尸检结果均提示骨骼矿化极度低下，4 d后因呼吸衰竭死亡。

These pathways include the production of advanced glycation end products, alterations in the sorbitol, hexosamine and protein kinase C pathways and activation of poly-ADP ribose polymerase.

代谢和血管通道的缺陷包括三个方面，一是糖基化终未产物形成，二是山梨醇、氨基己糖和蛋白激酶 C 改变，三是多聚二磷酸腺苷核糖多聚酶激活。

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化，CD44免疫组化法分析ADSCs表面标志；用含成骨、成脂诱导培养基培养4周后，行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

The cells were identified by invert phase contrast microscopy and alkaline phosphatase staining and mineralized nodules staining.The activity distribution of osteoblasts was determined by microtitration during a culture cycle.

通过倒置相差显微镜观察，并用碱性磷酸酶和矿化结节染色鉴定，利用微量滴定法测定一个培养周期的成骨细胞活性分布，探讨一种经济、高效的原代成骨细胞培养方法。

Results showed sTGC-CM, which is similar with the effect of hTGC-CM, was able to induce human DPSCs to make odontoblastic changes as indicated by remarkable morphological changes, increased proliferation ability, higher multipotential and colony forming capability, and expression of some odontogenic markers in gene and protein levels,such as DSP, DSPP, DMP1, BSP, OCN, OPN.

结果表明：人牙髓干细胞经过一定时间的猪牙胚条件培养基诱导后，能在细胞形态上和细胞周期上发生改变，能具有更高的克隆形成能力和多向分化能力，能维持更好的干细胞特性，具有更强的增殖能力，表达更高水平的碱性磷酸酶，免疫组化和RT-PCR提示诱导后的牙髓干细胞能在蛋白水平和基因水平表达出一些牙向分化的标志，如DSP、DSPP、DMP1、BSP、OCN、OPN等。

The biochemistry and image analyzing were used to investigate the effects of the PDGF-AA at high concentration on the osteoclastic bone resorption in the osteoblast-osteoclast co-culture system.

在高浓度PDGF-AA的作用下，应用生化检查和图象分析技术，检测PDGF-AA对成骨细胞-破骨细胞共育体系中破骨细胞骨吸收功能的影响；应用免疫荧光检测破骨细胞合成抗酒石酸酸性磷酸酶的变化；应用免疫组化和RT-PCR技术检测骨保护素配体的表达。

In this thesis, effects of common factors in yeast extract production with autolysis on yield and total amount of amino acid nitrogen in supernatant, degradation of RNA and saccharides during autolysis were studied, in which fresh baker\'s yeast was used as material. Extraction of 5\'-phosphodiesterase from barley rootlets was also studied. The optimum technology conditions of yeast extract production was determined by comprehensive considering results of above-mentioned study.

本论文以新鲜面包酵母为原料，研究了自溶法生产酵母抽提物过程中的影响因素对得率、上清液氨基氮总量、自溶过程中RNA降解及糖类物质降解的影响，同时还对麦芽根中5'-磷酸二酯酶的提取进行了研究，综合以上研究结果优化了酵母抽提物生产的工艺条件；此外，从酵母自溶残渣中提取了酵母细胞壁多糖，并对该多糖的乳化性及酯化修饰进行了研究，取得了下述主要研究结果。1。

Technical parameters application ＊ egg and milk products including rich fat milk powder,cocoa milk powder, milk powder,eggwhiteetc.＊ food and plant juice including oats,chickenjuice,coffee,speedy dissolving tea,flavorign,protein,bean,peanut protein,protein solution etc.＊ sugar series including corn syrup,com starch,amylaceum,pectin barley sugear,pltassium sorbate etc.＊ pharmaceutical products including chinese medicine extract,pharmiglucin,vitamin,antibiltie,amylase,lipase,protease etc.＊ plastic resins including ab,abs emulsion,pollopas,phenolic resin,,melamac,polyethylene,pvc etc.＊ detergents including normal,high grade washing powder,soap power,soda ash,emulsifier,brightener,phlphoric agent etc.＊ chemical products including sodiumfluoride,basis dye/colour,dye intermediate,compoumd fertilizer,catalyzer,sulphuric agent,amino acid and white carbon etc.＊ ceramics including aluminum oxide,ceramic tile material,magnesium oxide,speckstone etc.＊ others including aluminum hydroside,calcium chloride,staric acid agent etc.

料液桶d皿mof，awmatehalliquid 应用食品工业蛋奶制品富脂奶粉、胳肮，可可奶粉、代乳粉、蛋清等食物及植物汁燕麦鸡汁，咖啡，速溶茶，调味香料肉，蛋白质，大豆，花生蛋白质、蛋白质水解物等糖类玉米浆、玉米淀粉，葡萄糖，果胶、麦牙糖，山梨酸钾等化学工业氟化钠、碱性染料颜料、染料中间体、复合肥、甲茎硅酸、催化剂、硫酸剂、氨基酸、白碳黑等医药用品中药浸膏、药胶、酵母、维生素、抗生素、淀粉酶、脂肪酶、蛋白酶等塑料树脂 ab、abs乳液、尿醛树脂、酚醛树脂、密胶甲醛树脂、聚乙烯、聚氯乙烯等洗涤剂高级洗衣粉，普通洗衣粉、肥皂粉、苏打粉、乳化剂、增白剂、磷酸剂等陶瓷氧化铝、态砖材料、氧化镁、滑石等其它氢氧化铝、氯化钙、硬脂酸剂等冷却喷雾技术参数

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。