磷酸化酶

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

This key reaction is catalyzed by Ribulose 1,5-bisphosphate carboxylase/oxygenase, which is the most abundant protein on earth.

这步关键反应被1，5-二磷酸核酮糖羧化／加氧酶( Ribulose 1，5-bisphosphate carboxylase/oxygenase ，Rubisco)所催化，该酶是世界上最丰富的蛋白。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

Growth curve of the cells revealed the presence of strong proliferation activity.

观察细胞的生长状态及形态，绘制生长曲线并计算细胞倍增时间，并进行碱性磷酸酶染色和矿化能力的检测。

The present study explored the effects of morphine on plasticity of spinal cord in three parts as follows: 1. the effects of morphine on dorsal root terminal in spinal lamina Ⅱ of spared root rat---- a quantitative study by FRAP histochemistry; 2. the effects of morpnine on synaptic reinnervation in spinal lamine Ⅱ of spared root rat----an EM quantitative study and 3. the effects of morphine on collateral sprouting of nerve fiber in spared root of rat----an EM quantitative study

本课题从三个方面探讨了吗啡对脊髓可塑性的影响：1、吗啡对备用根大鼠脊髓Ⅱ板层背根终末的影响——抗氟化物酸性磷酸酶组化定量研究；2、吗啡对备用根大鼠脊髓Ⅱ板层突触重建的影响——电镜定量研究；3、吗啡对大鼠备用根神经纤维侧支出芽的影响——电镜定量研究。

Sequencing revealed that the most common genes appearedin the library is photorespiratory-related genes, such as ribulose bisphosphate carboxylase, Rubisco activase, glyoxylate aminotransferase, glutamine synthetase,glyceraldehyde dehydrogenase, ferredoxin, transport protein, etc.

测序之后发现最为显著的是一些光呼吸相关的基因如核酮糖-1，5-二磷酸羧化／加氧酶、Rubisco激活酶、乙醛酸转氨酶、谷氨酸转氨酶、甘油醛脱氢酶、铁氧还原蛋白和转运蛋白等。

The first step of the plant pterin branch is catalyzed by GTP cyclohydrolase Ⅰ and the first step in the conversion of chorismate to pABA is mediated in plants by aminodeoxychorismate synthase.

叶酸分子是由喋呤和对氨基苯甲酸从头合成的，二者合成的关键酶腺苷三磷酸环化水解酶和氨基脱氧分支酸合成酶已得到克隆鉴定。

The laboratory findings of a typical CCML patient comprised of peripheral blood leukocytosis, basophilia and eosinophilia,myeloid differentiation in different stages, and increased megakaryocytes. The immunohistochemical features of the CCML consisted of highly positive MPO and CD68, significant lowering of neutrophil alkaline phosphatase, positive for Philadel- phia chromosome or chimeric BCR/ABL gene, etc. But in most cases of juvenile CCML, the Philadelphia chromosome could not be detected.

临床表现以乏力、低热、贫血、肝脾及淋巴结肿大为主要特点；典型儿童慢性髓细胞白血病的实验室特点有白细胞计数高、嗜酸和嗜碱性粒细胞增多、骨髓不同阶段髓细胞分化和巨核细胞增多；免疫组化提示CD68，髓过氧化物酶，中性粒细胞碱性磷酸酶积分明显减低，Ph染色体或BCR/ABL融合基因阳性，而幼年型CCML的Ph染色体常为阴性。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织，用Percoll分离液分离出骨髓中的单个核细胞，在含体积分数为10%小牛血清的高糖DMEM培养液中，置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养，通过传代培养扩增骨髓基质干细胞，传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养，用倒置显微镜、HE染色观察增殖和分化情况，并测定碱性磷酸酶活性和钙结节形成能力。

A typical area of habitat fragmentation in the Thousand-Island Lake, Zhejiang Province, was selected as subject to study effects of habitat variation as a result of island forming, on arbuscular mycorrhizae infection rate and its correlation with phosphatase activity in the rhizosphere of Castanopsis sclerophylla.

以浙江千岛湖典型生境片断化区域苦槠为材料，研究了水库形成后岛屿化导致的群落生境差异对苦槠AM侵染率的影响以及AM侵染率与根际土壤磷酸酶活性的相关性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。