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Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂(β-甘油磷酸钠,地塞米松,维生素C)、成脂肪细胞诱导液(地塞米松,甲基异丁酸黄嘌呤,牛胰岛素,吲哚美辛)及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化,经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物(碱性磷酸酶、骨钙素mRNA、钙结节)、脂肪标志物(脂滴、PPARγ-2mRNA)、以及类神经标志物(尼克氏体、神经烯醇化酶、神经丝蛋白)。

Then the contents of brain tissue water, malonyl diadehyde and the activities of Na+-K+-adenosine triphosphatase (Na+-K+-ATPase), Ca2+-Mg2+-adenosine triphosphatase (Ca2+-Mg2+-ATPase), CuZn-superoxide dismutase and mitochondrial Mn-superoxide dismutase were measured.

测定脑组织含水量、丙二醛含量、钠钾三磷酸腺苷酶(Na~+-K~+-ATPase)、钙镁三磷酸腺苷酶Ca~(++-Mg~-ATPase、铜锌超氧化物歧化酶以及线粒体锰超氧化物歧化酶的活力。

Taking zinc oxide and phosphoric acid as raw materials and silane coupling agent as modified agent,the micro-size zinc phosphate is prepared by wet-process mechanicochemical method and its modification is carried out.

以氧化锌和磷酸为原料,以硅烷偶联剂为改性剂,采用机械力化学湿法制备微细磷酸锌并进行表面改性。

Staining observation on complex mesochondrium of marrow-derived mesenchymal stem cells-TCP: It was found with TB staining that the complex of marrow-derived mesenchymal stem cells-TCP inside the joint cavity obviously had positive staining, which indicated that there were glycosidoprotein base formed.

骨髓基质干细胞-磷酸三钙复合体软骨基质特染观察结果:甲苯胺蓝染色发现,植入关节腔的骨髓基质干细胞-磷酸三钙复合体有明显阳性染色,表明有糖蛋白基质形成。

On day 8 of pregnancy, STAT3 immunostaining was mainly located in the decidual area near the lumen, and tyrosine-phosphorylated STAT3 immunostaining was seen in the decidual area near the myometrium, being stronger in the mesometrium side.

妊娠第8天时,STAT3免疫染色主要位于近腔蜕膜区,而酪氨酸磷酸化的STAT3主要位于近肌层蜕膜区,在子宫系膜侧的蜕膜中酪氨酸磷酸化STAT3的信号较强。

Key words: sludge of wet-process phosphoric acid; comprehensive utilization; monoammonium phosphate MAP

关键词]湿法磷酸淤浆;综合利用;磷酸一铵

China's current phosphate demand is some 10 Mt/a(P2O5) and the same will be kept still in 2010. So the adjustment of product mix should be emphasized at the enhancement of monoammonium phosphate, diammonium phosphate and NPKs, maintenance of FMP and decrease of SSP.

目前我国化学磷肥的年需求量约1 000万吨P2O5,到2010年前后我国化学磷肥的年需求量仍为1 000万吨P2O5左右,提出我国磷肥产业结构的调整应当积极发展磷酸一铵、磷酸二铵和三元复合肥,稳定钙镁磷肥的生产,适当压缩过磷酸钙的生产。

The current standard for monoammonium phosphate is ZB/TG 21009-90,whenused in our products, disputation occurred against the analysis results for many times because of its defects.

摘 要]粉状磷酸一铵产品质量标准为ZB/TG21009-90,在执行该系列标准对我厂磷酸一铵产品质量进行检验的过程中,多次发生分析结果的争执。

The separation was achieved within 4 min using a BEH Shield RP18 column (100 mm 2.1 mm, 1.7 μm) with 50 mmol/L monoammonium phosphate solution (pH 6.5) and acetonitrile as the mobile phase.

以BEH Shield RP18柱(100 mm 2.1 mm,1.7 μm)为分析柱,以50 mmol/L的磷酸二氢铵(pH 6.5)和乙腈为流动相进行梯度洗脱,3种磷酸腺苷在4 min内实现了较好的分离。

The results showed that CRMP had higher phosphorus availability in soil and the changes ofavailable P concentration in both root zone and non-root zone soil were more reasonable during the sorghum growing season as compared to common monoammonium phosphate.

与普通磷酸一铵相比,控释磷酸一铵施入土壤后磷的有效性较高,无论是近根区还是非根区土壤有效磷随时间的变化分配更趋于合理。

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