磷酸

Methods: The chromatographic separation was performed on a Hypersil ODS2 column(250 mm×4.6 mm, 5 μm) with mobile phases of potassium dihydrogen phosphate hexyl amine solution-acetonitrile(74∶26) for the determination of its related substances and methanol-0.05 mol/L potassium dihydrogen phosphate solution(1∶1) for the determination of dexbetamethasone sodium phosphate. The flow rate was 0.8 ml/min, the detection wavelength was 254 nm, the column temperature was 25℃ and the sample size was 20 μl.

色谱柱为Hypersil ODS2柱(250 mm×4.6 mm，5 μm)；有关物质测定的流动相为磷酸二氢钾己胺溶液-乙腈(74∶26)，含量测定的流动相为甲醇-0.05 mol/L磷酸二氢钾溶液(1∶1)；流速为0.8 ml/min，检测波长为254 nm，柱温为25℃，进样量为20 μl。

Tissue samples were examined on 0, 7, 14, 21, 28 and 35 d with micro section paraffin and histochemistry staining method.

分别于0，7，14，21，28和35d取材，利用显微石蜡切片、组织化学染色的方法检测其肝、消化道和肌肉细胞糖原、酸性磷酸酶和碱性磷酸酶细胞化学特征。

Tissue samples were examined on 0, 7, 14, 21, 28 and 35day with micro section paraffin and histochemistry staining method.

分别于0、7、14、21、28和35 d取材，利用显微石蜡切片、组织化学染色的方法检测其肝、肠和肌肉细胞糖原、酸性磷酸酶和碱性磷酸酶细胞化学特征。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

In this paper,the process of sodium hypophosphite production and equipment is summarized,new views and suggestions are put forward according to corresponding technological exploitation.

王书林，沈万涛，次磷酸钠生产工艺及反应器的优化对生产次磷酸钠工艺及主要设备进行了评述，对相应的技术开发提出了新的见解和建议。

Owing to phosphorous acid is produced from phosphorus trichloride in industry by hydrolysts, inspissation and crystallization, if we use phosphorus trichloride directly on synthesize N- iminodiacetic acid, not only can simplify operation, but also can red.

原工艺采用的是在酸性条件下高温时将甲醛滴加到亚磷酸与亚氨基二乙酸一钠盐混合溶液中进行反应，考虑到工业上亚磷酸的合成一般采用三氯化磷水解、提浓、结晶制得，直接将三氯化磷用于双甘膦的合成，不仅可以简化操作过程，而且对于降低生产成本和能耗有明显意义。

Originally techniques dropped formaldehyde into the mixture of phosphorous acid and monosodium of imino diacetic acid in high temperature. Owing to phosphorous acid is produced from phosphorus trichloride in industry by hydrolysis, inspissation and crystallization, if we use phosphoros trichloride directly on synthesize N- iminodiacetic acid, not only can simplify operation, but also can reduce the cost and save energy.

原工艺采用的是在酸性条件下高温时将甲醛滴加到亚磷酸与亚氨基二乙酸一钠盐混合溶液中进行反应，考虑到工业上亚磷酸的合成一般采用三氯化磷水解、提浓、结晶制得，直接将三氯化磷用于双甘膦的合成，不仅可以简化操作过程，而且对于降低生产成本和能耗有明显意义。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

Examined the prospect of multiple kenaf forage harvests during a single growing season, harvesting kenaf at 85, 92, and 99 DAP and then reharvesting the same plants at 179 DAP.

1995检验红麻前景多在单一种植牧草丰收季节，在收割红麻85、92、 99、磷酸二铵reharvesting然后在同一厂磷酸二铵179。

We will synthesize NO2A, PhNO2A and its dimer structure, and study the stability constants for lanthanides complexes and kinetics in hydrolyzing BNPP. And we also want to understand their differences in catalytic mechanism.

由前人的研究可得知，改变配位子的特性，或许可提高金属错合物对於磷酸酯键的水解效力，因此我们将比较NO2A 及 PhNO2A 对於镧系金属稳定常数，及其镧系金属错合物水解磷酸酯键的影响。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。