磷酸

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

A variety of factors were comparatively studied with regard to their effects on the stability of phosphoglucomutasein Chinese cabbage.

首先对影响葡萄糖磷酸变位酶稳定性的因素进行了比较研究，结果发现，在所有被检测的因子中，磷酸根对酶的保护作用最强，而Tris－盐酸则效果极差。

Acid phosphatase, alkaline phosphatase, and superoxide dismutase were found in the serum, blood cells and liver extract of Chlamys farreri . The activities of ACP and ALP were highest in the liver.

在栉孔扇贝的血清、血细胞和肝脏提取液中均发现有酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶活性，其中，ACP和ALP在肝脏中的活性最高。

In this study, preparation of chitosan molecular imprinted resins were studied, and a simple method with a nozzle was developed, in which molecularly imprinted chitosan resin was made for hemoglobin as"print"molecule and chlorohydrin was used as crosslinker.

采用滴加成球法制备出球形壳聚糖介质，研究了三聚磷酸钠溶液浓度及空气流速对成球的影响，得到其最佳条件为：三聚磷酸钠溶液浓度3％；空气流量为1.8m〓／min，线速度为23.81m／h。

Water、alcohol denat.、glycerin、butylene glycol、alanine、sucrose、urea、peg-60 hydrogenated castor oil、triethanolamine、allantoin、limonene、hamamelis virginiana extract、linalool、menthoxypropanediol、propylene glycol、alpha-isomethyl ionone、aspartic acid、acrylates/c10-30 alkyl acrylate crosspolymer、bisabolol、citronellol、fructose、glucose、glutamic acid、hexylene glycol、dextrin、hexyl nicotinate、fragranceparfum

水、双丙甘醇、变性乙醇、甘油、聚乙二醇-75、丁二醇、麦芽糖醇、PEG-60氢化蓖麻油、透明质酸钠、聚山梨醇酯-20、杏汁、乙醇、乳酸菌/梨汁发酵过滤液、磷酸氢二钠、乙基葡糖苷、羟丙基甲基纤维素、磷酸钾、黄原胶、豆瓣菜提取物、EDTA二钠、香精、茶叶提取物、苯氧乙醇。

Synthesis of fatty acid alkylolamide phosphate using cocoanut oil,methanol and monoethanolamide as material.

以椰子油、甲醇和单乙醇胺为原料合成椰子油脂肪酸烷醇酰胺，然后经磷酸化及中和反应制得椰子油脂肪酸烷醇酰胺磷酸酯盐。

The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术，观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响，结果证实了促孕液治疗卵巢静止、持久黄体的机理：(1)微弱的雌激素样作用，能刺激卵泡发育，促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化；(2)提高子宫内膜碱性磷酸酶的活性；(3)改善微循环；(4)促进子宫运动。

The steps for preparing insecticide include: first shaking flask for fermentation, adding amidon, soya bean cake powder, cotton cake powder, protease leather, yeast powder, maize milk, magnesium sulfate heptahydrate, monobasic potassium phosphate, dibasic potassium phosphate, calcium carbonate alpha-amylase and make-up water by ratio in culture medium with 100 unit volumes; secondarily filling 100ml culture mediums in triangular bottle, then seeding BTH-13 after disinfection and culturing them until the brood-cell pulls off under finite temperature; third adopting the selected culture medium and fermenting them in full-automatic fermentation tank, acidifying the fermentation liquor after the fermentation, adding the emulsifier, and directly performing spray-drying after the compression of the fermentation liquor to prepare the wettable powder.

制备杀虫剂的步骤是：首先是摇瓶发酵，在100单位体积的培养基中，按比例加入淀粉、豆饼粉、棉饼子粉、蛋白胨、酵母粉、玉米浆、七水硫酸镁、磷酸二氢钾、磷酸氢二钾、碳酸钙、及α-淀粉酶，补充水；其次是在三角瓶中装100ml培养基，灭菌后接种BTH-13，在一定温度培养至芽孢脱落；第三是采用筛选出来的培养基，在全自动发酵罐中进行发酵，发酵结束后发酵液酸化，加入乳化剂，酵液浓缩后直接喷雾干燥制备可湿性粉剂。

To investigate the prevention of anti-apoptosis drugs (sodium ferulate, SF; schisandrin B, Sch B; flos chrysanthemi, FC; semem planagines, SP) on lens epithelial cell damaged by oxidative stress, and to study the changes of intracellular-free calcium, cyclic adenosine monophosphate and cyclic guanine monophosphate, in order to disclose the mechanism of signal transduction in cellular and molecular level.

目的 本研究旨在探讨细胞凋亡抑制剂阿魏酸钠、五味子乙素、菊花、车前子对氧化损伤的晶状体上皮细胞内Ca2+、环磷酸腺苷、环磷酸鸟苷的影响，从细胞信号转导角度揭示天然药物防治白内障的细胞和分子学机制。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。