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Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明,AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化;TUNEL检测呈阳性,说明有染色体DNA出现断裂;Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露;电镜观察的结果显示,转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

With either method, first dissolve or suspend 10.0 g of the specimen if it is a solid, or 10 mL, accurately measured, if the specimen is a liquid, in pH 7.2 Phosphate Buffer, Fluid Soybean–Casein Digest Medium, or Fluid Casein Digest–Soy Lecithin-Polysorbate 20 Medium to make 100 mL.

不管使用哪一种方法,首先将10.0g固体样品或精密量取的10mL液体样品,放入pH 7.2的磷酸盐缓冲液,或液体大豆酪蛋白消化物培养基,或液体酪蛋白消化物-大豆卵磷脂-聚山梨酯20中,制成100mL的溶液或悬浮液。

For the mieroemulsions, Miglyol 812 was chosen as oil phase, SbPC and HS-15~ as surfactants, PEG400 and ethanol (7:3) as cosurfactant and the double-distilled water as water phase. Pseudo-ternary phase diagrams were constructed to obtain the concentration range of each component for the microemulsion formation. The effects of various ratio of SbPC to HS-15 and different weight ratios of surfactant to cosurfactant on the droplet size were investigated, as well as viscosity and stability of microemulsion. The constitute of stable blank ME with more oil and little surfactant was confirmed.

分别以IPM和Miglyol 812作为油相、豆磷脂与HS-15为表面活性剂、PEG 400和乙醇作为助表面活性剂、重蒸水作为水相,绘制伪三元相图,研究各组分的组成对微乳形成的影响,并考察了不同油相、不同表面活性剂、助表面活性剂的组成变化对制剂粒径、粘度及稳定性的影响,确定了含有较多油相、较少表面活性剂的稳定空白微乳的组成。

INGREDIENTS: skim milk, sugar, cream, chocolaty chips (sugar, coconut oil, cocoa processed with alkali, partially hydrogenated coconut oil, cocoa, salt, soy lecithin, natural flavor), corn syrup, cocoa processed with alkali, whey protein, egg yolks, tapioca maltodextrin, soy protein, natural flavor, Propylene Glycol Monostearate, guar gum, carrageenan, cellulose gum, mono and diglycerides, salt, malt extract, xanthan gum, molasses, vitamin A palmitate, dextrose, caramel color

成分:脱脂牛奶,糖,奶油, chocolaty芯片(糖,椰子油,可可碱处理,部分氢化的椰子油,可可粉,盐,酱油,卵磷脂,天然香料),玉米糖浆,可可碱处理,乳清蛋白,蛋蛋黄,木薯麦芽糊精,大豆蛋白,天然香料,丙二醇酯,瓜尔豆胶,卡拉胶,纤维素胶,单声道和diglycerides ,盐,麦芽提取物,黄原胶,糖蜜,维生素A棕榈酸酯,葡萄糖,焦糖色

Both diabectic angiopathy and lipid soakage are presented in group DMCH.Conclusion It was confirmed that there was rather high rate of cholecysts stone forming in the rabbits with DM led by Alloxan,that the bile acid,phospholipid reduced and cholesterol increased in the

证实四氧嘧啶致糖尿病大耳白兔胆囊结石形成率甚高,胆囊胆汁酸分泌减少、磷脂减少和胆固醇增加形成成石胆汁,以及形成胆囊微血管病变是胆石形成的重要因素,为深入研究提供了有用的工具。

Conches (from the Spanish word "", meaning a shell) is the name given to the troughs in which 100 to 1000 kilograms of chocolate paste at a time can be heated up to 80 °C and, while being constantly stirred, is given a velvet smoothness by the addition of certain amounts of cocoa butter and of the very valuable lecithin.

巧克力揉捏机(源于西班牙词&concha&,是海螺的意思),它可以同时容纳搅拌100到1000公斤的巧克力泥,并加热到80 °C,在向其中添加可可脂和卵磷脂之后,使之变得柔滑细腻。

Before and after the treatment with corticoid plus small dose of heparin for 4~6 weeks,the serum ACA was measured by ELISA method in 20 nephrotic syndrome children and the results were compared with those obtained from 40 normal children.

近年来有研究报道抗心磷脂抗体( anti- cardiolipin antibody,ACA)在成年人原发性肾病综合征的发病及发展中可能有着重要的作用,但在儿童原发性肾病综合征小剂量肝素钙治疗前和治疗后 ACA检测其高凝状态未见报道。

Substantial amounts of dialysable "phosphatido-peptides" have also been found.

还发现大量的可透析的&磷脂--肽&。

The effect of aluminum ions on the Fe2+-initiated lipid peroxidation in liposomes was studied by chemiluminescence, TBARS, conjugate dienes assays and ESR spectroscopy.

利用化学发光、TBA 反应与测量共轭二烯的方法观测了Al3+对Fe2+启动的卵磷脂脂质体脂质过氧化的影响。

The exocytosis of milk fat may be related to its high lysolecithin content.

乳脂的外吐,可能与它溶血卵磷脂含量有关。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。