碳酰亚胺

For the advantages of mild reaction conditions,DDC condensation method and acyl chloride esterification method are much more popular.

由于具有反应条件温和的优点，二环己基碳二亚胺脱水缩合法和酰氯酯化法是目前国内外最常用的两种方法。

Methods GAMP was activated with cyanogen bromide and combined with 1, 6-adipic acid dihydrazide. The prepared polysaccharide derivative GAMP-ADH was coupled to recombinant protein Pep10 with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride.

GAMP经溴化氰活化后，共价接合己二酰肼手臂，在碳二亚胺催化下与重组蛋白Pep10偶联，制备结合物GAMP-ADH-Pep10。

The invention relates to a preparation method for carbon nanotubes modified by micromolecule imide, and belongs to the material technical field.

本发明属于材料技术领域，具体涉及一种小分子酰亚胺修饰的碳纳米管的制备方法。

Was synthesized from cyanoacetic acid and diethylamine via DCC coupling under mild conditions.

中间体5 的制备由氰基乙酸在二环己基碳二亚胺作用下经酰胺化一步制得，条件温和，收率较高，避免使用剧毒的氰化钠。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Tetrachloroterephthalic acid was prepared by chlorinating terephthalic acid, oleumsolvent and iodine catalyst. Tetrachloroterephthalic acid was acylated by sulphurous oxychloride in the presence of DMF to form tetrachloroterephthaloyl chloride. The acyl chloride product was fluorinated by potassium fluoride and esterified by methanol to synthesize dimethyl tetrafluoroterephthalate. The ester was reduced with potassium borohydride and lithium chloride to give tetrafluoroterephthalyl alcohol. The above product was bromated in the hydrobromic acid to obtain 4-bromomethyl-2,3,5,6-tetrafluoro-benzene methanol. 4-Methyl-2,3,5,-6-tetrafluorobenzene ethanol was obtained by reducing the bromide with magnesium. In the exist of DCC and DMAP, tefluthrin was got by the condensation reaction between 4-methyl-2,3,5,6-tetrafluoro-benzene methanol and-(1RS,3RS)- 3-(2- chloro-3,3,3-trifluoro-1-propenyl)-2,2-dimethyl cycolpropanecar-boxylate acid.

对苯二甲酸与氯气在碘存在下以发烟硫酸为溶剂进行氯化制备四氯对苯二甲酸；再以DMF为催化剂与二氯亚砜反应生成四氯对苯二甲酰氯；以氟化钾为氟化剂，环丁砜为溶剂置换氟化，再与甲醇酯化得到四氟对苯二甲酸甲酯；以KBH4-LiCl为还原剂二甲醚为溶剂反应制得四氟对苯二甲基苄醇；以氯苯为溶剂在溴化氢溶液中溴化得到溴化产物；在乙醇溶液中经镁粉还原得到4-甲基-2，3，5，6-四氟苄醇；甲基苄醇与功夫酸在催化剂二环己基碳二亚胺和4-二甲氨基吡啶作用下缩合得到七氟菊酯，总收率达43.6%。

Methods Activate groups A and C meningococcal polysaccharide with cyanogens bromide and prepare into MenAPS-ADH and MenCPS-ADH derivatives by reacting with 1, 6-adipic acid dihydrazine. Mix MenAPS-ADH and MenCPS-ADH derivatives with purified tetanus toxoid at a certain mass ratio, then add l-ethyl-3(3-dimethylaminopropyl)-carbodiimide to prepare bulks of MenAPS-TT and MenCPS-TT conjugates respectively. Mix the two bulks at a certain ratio, add stabilizer and lyophilize to prepare group A+C meningococcal polysaccharide conjugate vaccine.

A群和C群脑膜炎球菌多糖在溴化氰的活化下，以1，6己二酰肼作为连接子，与精制破伤风类毒素在碳二亚胺作用下结合，再按一定的比例将两者混合，制成A+C群脑膜炎球菌多糖结合疫苗原液，加保护剂冻干后，制成A+C群脑膜炎球菌多糖结合疫苗。

Chlorine atoms were selectively introduced into the chlorin ring based on different reactivity between vinyl group at 3-position and 20-meso position by electrophilic addition and electrophilic substitution using hydrogen peroxide/hydrochloric acid, N-chlorosuccinimide, hydrogen chloride (or concentrated hydrochloric acid) as chlorinating reagent.

以过氧化氢/盐酸、N-氯代丁二酰亚胺、氯化氢为氯化剂，根据3-乙烯基和20-meso-位的不同化学反应活性，通过亲电加成和亲电取代反应，在二氢卟吩环上选择性地引进氯原子，完成8种未见报道的具有叶绿素-a基本碳架的氯代二氢卟吩衍生物的合成。

Peptides are traditionally synthesized in liquid phase, such methods are DCC, mixed anhydrides, acyl chloride and azide etc., which provide more choices for peptides synthesis.

传统的肽类合成多采用液相化学合成，常用的包括碳二亚胺法，混合酸酐法、酰氯法、叠氮物法等等，为多肽的合成提供了多种选择。

Thus if we control the reaction temperature at somesolvent we can obtain the products either by the reaction of phosphoryl-stabilizedcarbonanion with N-tosyl imines or by the reaction of methoxycarbonyl-stabilizedcarbanion with N-tosyl imines.

在一定溶剂中，如果控制反应的温度可以控制得到由膦酰基稳定的碳负离子和亚胺反应或由甲氧羰基稳定的碳负离子和亚胺反应。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。