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HPLC was coupled with both mass spectroscopy and ultraviolet absorption spectroscopy for simultaneous separation and characterization of the products from glow plasma reaction of chloroform.(3) Phosphorus pentachloride has been used to perchlorinate acenaphthene under moderate pressure and temperature in sealed reaction vessels.

在此基础上，以反相C〓柱为固定相，甲醇/乙醇/环己烷为流动相，采用梯度洗脱的方法，对氯仿辉光放电合成产物进行高效液相色谱-紫外光谱-质谱联用分离分析，成功的分离得到包括富勒烯C〓和全氯代碳簇在内的数十种产物，并得到了相应的质谱和光谱数据。

Finally, two short-path distillation processes and one falling film evaporator are applied in the regeneration of waste lubricating oil. In the test, the diesel oil, gasoline and lubricating oil are distilled respectively, while the gelatinoid materials, asphaltene and carbon residue remain in the draft. The regenerative lubricating oil accords with the standards of base oil. And the recovery efficiency is up to 75 percent. The technology used in the test doesnt comprise processes like pickling and alkali neutralization, which would cause secondary pollution like acid draff and sulfur dioxide and so on. As a result of this, our technology is friendly to the environment.

最后，利用一级薄膜蒸发和两级短程蒸馏工艺对废润滑油进行了再生回收工业应用试验，将柴油、汽油等轻油以及润滑油馏分分别蒸馏出来，而大部分胶质、沥青质、碳垢存在于残渣中，试验得到的再生润滑油符合基础油的标准，回收率达到75％，达到废润滑油再生的目的；同时再生过程中没有酸洗、碱中和等工序，不产生酸渣、二氧化硫等二次污染，是一种清洁环保的废润滑油再生工艺。

In this area seven microfacies in the Chuangde Fm. red beds can be separated. They are: 1 reddish foraminifera grainstone; 2 reddish microfossils packstone/wackestone; 3 reddish marlstone with microfossils; 4 reddish marlstone; 5 red to variegated floatstone ; 6 reddish shales; 7 reddish radiolarite/ radiolarian chert.In the Yamudok Cuo area, it can be distinguished six microfacies in the redbeds including 1 reddish foraminifera grainstone; 2 reddish microfossils marlstone; 3 reddish shales; 4 yellow-grayish floatstone ; 5 reddish radiolarian chert; 6 reddish chert with radiolaria. The depositional environment of the Chuangde formation can be interpreted from sediments composition, as is deep oceanic basin adjacent to continental slope/rise. The slope/rise setting is indicated by the occurrence of olistoliths and slumped pelagic/hemipelagic limestone, present near the top of the Chuangde Fm.

进一步资料对比研究表明，全球上白垩统海相红层具有以下特征：a上白垩统红层出现在北大西洋、阿尔卑斯、喀尔巴阡、土耳其，向东一直到特提斯喜马拉雅地区和澳大利亚西北角的Exmouth海底高原，横贯整个特提斯和北大西洋；b沉积环境以外陆棚-斜坡和盆地为主，在斜坡环境下多发育有浊流沉积，如Scaglia Rossa和Nietrental组；c有机碳含量普遍很低，0～0.3％之间；d盆地相仅发育红色泥质岩，而斜坡和远洋环境下不仅发育红色页岩，同时还有红色泥灰岩、红色灰岩和红色硅质岩；e北大西洋和地中海地区在Cenomanian-Turonian界线黑色页岩之后不久的Turonian早期就出现红色沉积，而藏南出现在Santonian早期，Exmouth出现在Campanian早期，红层出现似乎有"西早东晚"的趋势。

Cancellous bone from the lumbar proteomic expression analysis show that actin, keratin, enolase, ATP synthase, the expression of myosin may be related to ovarian lumbar cancellous bone after osteoporosis-related happened; Qiang Gu Bao Po on the lumbar spine osteoporosis intervention effect may be related to reduced carbonic anhydrase, actin, crystal protein, 3- phosphoglycerate dehydrogenase, serum albumin, ATP synthase, myosin, the enolase expression.

从腰椎松质骨蛋白质组表达分析可知，肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白的表达增强可能与去卵巢后腰椎松质骨骨质疏松的发生相关；强骨宝对腰椎骨质疏松的干预效应可能与下调碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶的表达有关。

The main products include: large type heating furnace of continuous walking type furnace and annular furnace、 Tilting Aluminum Melting、holding temperature furnace、 homogenization furnace、 annealing furnace、quenching furnace、aging furnace used for aluminum material;copper material of heating furnace、 annealing furnace（brightness/commonly）、reverberator etc; heating furnace、quenching furnace、tempering furnace、drying furnace、sintering furnace、carburizing nitriding furnace、aging furnace、quenching and high tempering furnace、normalizing furnace、galvanizing line and color coating line used for steel;sand core surface drying furnace、casting annealing furnace used in automobile generator profession;the main type furnaces include: pusher-type furnace、roller- type furnace、rotary- type furnace、 pit type furnace、 mesh-belt furnace、well- ty

公司的主要产品包括：步进炉、环行炉等大型加热炉；铝材方面的熔铝炉、保温炉、加热炉、均质炉、退火炉、固熔炉、时效炉等；铜材方面的加热炉、退火炉、反射炉等；钢铁方面的加热炉、淬火炉、回火炉、烘干炉、烧结炉、渗碳炉、氮化炉、时效炉、调质炉、正火炉、镀锌线、彩涂线等；汽车发动机行业的砂芯表干炉、铸件退火炉等；主要炉型有推杆炉、辊底炉、转底炉、坑式炉、网带炉、井式炉、燃油炉、箱式炉、台车炉、多用炉、钟罩炉、真空炉等。

The main products include: large type heating furnace of continuous walking type furnace and annular furnace、 Tilting Aluminum Melting、holding temperature furnace、 homogenization furnace、 annealing furnace、quenching furnace、aging furnace used for aluminum material;copper material of heating furnace、 annealing furnace（brightness/commonly）、reverberator etc; heating furnace、quenching furnace、tempering furnace、drying furnace、sintering furnace、carburizing nitriding furnace、aging furnace、quenching and high tempering furnace、normalizing furnace、galvanizing line and color coating line used for steel;sand core surface drying furnace、casting annealing furnace used in automobile generator profession;the main type furnaces include: pusher-type furnace、roller- type furnace、rotary- type furnace、 pit type furnace、 mesh-belt furnace、well- type furnace 、gas fired furnace、 box type furnace、trolley furnace、multi-purpose furnace、 bell-type furnace 、vacuum-type furnace and so on.

公司的主要产品包括：步进炉、环行炉等大型加热炉；铝材方面的熔铝炉、保温炉、加热炉、均质炉、退火炉、固熔炉、时效炉等；铜材方面的加热炉、退火炉、反射炉等；钢铁方面的加热炉、淬火炉、回火炉、烘干炉、烧结炉、渗碳炉、氮化炉、时效炉、调质炉、正火炉、镀锌线、彩涂线等；汽车发动机行业的砂芯表干炉、铸件退火炉等；主要炉型有推杆炉、辊底炉、转底炉、坑式炉、网带炉、井式炉、燃油炉、箱式炉、台车炉、多用炉、钟罩炉、真空炉等。

Methods Human PDLC cultured in vitro was collected and seeded on Col, HA, HA/Col scaffolds crosslinked by carbodiimide. The influences of scaffolds on cell adhesion and growth were observed by MTT assay. The growth of human PDLC on scaffolds was observed through inverted phase contrast microscope and scanning electron microscope.

将体外培养的人牙周膜细胞接种到碳化二亚胺交联的胶原、透明质酸及透明质酸/胶原支架上；MTT法检测支架对人牙周膜细胞黏附、生长的影响；并用倒置相差显微镜和扫描电镜观察形态变化。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

In this area seven microfacies in the Chuangde Fm. red beds can be separated. They are: 1 reddish foraminifera grainstone; 2 reddish microfossils packstone/wackestone; 3 reddish marlstone with microfossils; 4 reddish marlstone; 5 red to variegated floatstone ; 6 reddish shales; 7 reddish radiolarite/ radiolarian chert.In the Yamudok Cuo area, it can be distinguished six microfacies in the redbeds including 1 reddish foraminifera grainstone; 2 reddish microfossils marlstone; 3 reddish shales; 4 yellow-grayish floatstone ; 5 reddish radiolarian chert; 6 reddish chert with radiolaria. The depositional environment of the Chuangde formation can be interpreted from sediments composition, as is deep oceanic basin adjacent to continental slope/rise. The slope/rise setting is indicated by the occurrence of olistoliths and slumped pelagic/hemipelagic limestone, present near the top of the Chuangde Fm.

进一步资料对比研究表明，全球上白垩统海相红层具有以下特征：a上白垩统红层出现在北大西洋、阿尔卑斯、喀尔巴阡、土耳其，向东一直到特提斯喜马拉雅地区和澳大利亚西北角的Exmouth海底高原，横贯整个特提斯和北大西洋；b沉积环境以外陆棚-斜坡和盆地为主，在斜坡环境下多发育有浊流沉积，如Scaglia Rossa和Nietrental组；c有机碳含量普遍很低，0～0.3％之间；d盆地相仅发育红色泥质岩，而斜坡和远洋环境下不仅发育红色页岩，同时还有红色泥灰岩、红色灰岩和红色硅质岩；e北大西洋和地中海地区在Cenomanian-Turonian界线黑色页岩之后不久的Turonian早期就出现红色沉积，而藏南出现在Santonian早期，Exmouth出现在Campanian早期，红层出现似乎有&西早东晚&的趋势。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。