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碳氧血红蛋白

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Objective To investigate factors affecting the determination of the percent carboxyhemoglobin saturation of blood in an attempt to offer further data for results interpretation and sample storage requirement.

目的 考察血中碳氧血红蛋白饱和度测定的影响因素,为其结果评定和所需样品保存条件提供实验依据。

This article had made self-matched test of 62 male adults which showed no significant statistical difference between the carboxyhemoglobin contents in the blood from finger and ear by the method of replacement with mercury.

血中碳氧血红蛋白的含量是评价环境中一氧化碳污染对人体健康影响的一种特异、灵敏的指标,因而在研究空气污染的危害时被广为采用。

The carboxyhemoglobin levels in artery blood were detected with CO-oximeter and the expression of intercellular adhesion molecule-1 (ICAM-1) in the lung was detected by Western blotting.

应用一氧化碳血氧分析仪监测动脉血中碳氧血红蛋白水平的变化;应用Western blotting检测肺组织中细胞间粘附分子-1(ICAM-1)表达的变化。

Carboxyhemoglobin level in the artery blood, malondialdehyde content and superoxide dismutase activity in the lung, heart, liver and kidney were detected. The 24-hour survival rate of rats was studied.

测定各组心、肺、肝和肾组织匀浆中丙二醛含量、超氧化物歧化酶活性及血液内碳氧血红蛋白的变化,观察动物 2 4小时存活率。

The carboxyhemoglobin levels of in-flowing pulmonary blood and out-going pulmonary blood were determined at 2,4 and 6 hours after treatments. Malondialdehyde contents and superoxide dismutase activities in the lung,liver and blood were also determined. Pathological changes in lung and liver were examined with light microscope,and immunohistochemical technique was used for analysis of heme oxygenase-1 (HO-1) protein expression and distribution in lung and liver.

各组分别在制模后2、4和6 h测定出入肺血中碳氧血红蛋白水平;肺、肝组织及血液中丙二醛含量及超氧化物歧化酶活性;光镜下观察肺、肝组织形态学改变;免疫组化分析血红素加氧酶-1(HO-1)在肺、肝组织中的蛋白表达和分布。

Mean carboxyhemoglobin leel in smokers was 3.81 +/- 2.17 gdL(-1) as compared to 2.95 +/- 1.33 gdL(-1) in non-smokers.

平均碳氧血红蛋白量吸烟组为3.81 +/- 2.17 gdL(-1),非吸烟组为2.95 +/- 1.33 gdL(-1)。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达,酶联免疫法检测培养上清液中碳氧血红蛋白含量,四唑氮比色法检测细胞增殖。

By addition of suitable amount of sodium hydrosulfite into blood samples which were diluted by 0.1% Na2CO3, the absorption curve from 400 nm to 600 nm and absorbance at 419 nm and 425.5 nm of the blood samples were detected for qualitative and quantitative determination of carboxyhemoglobin.

检血用0.1% Na2CO3稀释后加入连二亚硫酸钠,用紫外-可见分光光度计测定500nm-600nm吸收光谱图和419nm及425.5nm处的吸光度值,对碳氧血红蛋白进行定性和定量分析。

Long-term inhalation of Fio2 0.75 initially increasedand then gradually decreased exhaled CO to basal levels, concomitantwith a decrease of arterial carboxyhemoglobin.

长时间吸入75%氧气时在最初会增加呼出气一氧化碳的水平,但继之逐渐下降并伴有动脉碳氧血红蛋白的减少。

Investigations have already shown that HO can increase blood stream into uterus placenta, accelerate the formation of blood vessels, and protect placenta histiocyte. Enough blood stream into uterus placenta is the prerequisite for healthy growth of a fetus. Internal and external researches mainly focus on adults or

本研究通过检测健康足月儿及足月小于胎龄儿脐血中HO-1的活性,血浆碳氧血红蛋白的百分含量,并观察HO-1在脐血管上的表达,研究:⑴新生儿脐血中HO-1活性、HbCO百分含量及其与出生体重的关系;⑵新生儿脐

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