碱的

Macerate 1.0g of the powdered sample with 20.0 ml of chloroform plus 0.3ml of 25％ NH_4 OH in a glassstoppered flask overnight.

本文报道用不含粘合剂的氧化铝薄层，以二甲苯-丙酮-无水乙醇-二乙胺(50：40：10：0.6)为展开剂分离茄科植物中的莨菪碱、东莨菪碱、山莨菪碱、樟柳碱及红古豆碱等五种莨菪烷生物碱，用改良Dragendorff试剂-Wagner试剂(1：1)显色，定量收集莨菪碱、东莨菪碱及山莨菪碱的斑点，莨菪碱和东莨菪碱用氨甲醇洗脱，蒸干洗脱液后分别用pH 5.6及pH 4.5的溴甲酚绿溶液作比色测定；山莨菪碱用4％醋酐氯仿洗脱，蒸干洗脱液后用pH 5.6的溴甲酚绿溶液作比色测定。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Conclution: Diester aconitum alkaloid can be changed to monoester aconitum and aconine alkaloids. Under the controled condition benzoylaconine is a major hydrolysis products.

双酯型的乌头碱可水解转化为单酯型乌头碱和乌头原碱；在控制条件下，苯甲酰乌头原碱为主要水解产物。

The frequency of EST-SSRs was 6.3%. The dinucleotide repeat motif was the most abundant SSR, accounting for 71.8%, followed by 9.1%, 8.1%, 5.7% and 5.3%, respectively,for tri-, hexa-, penta- and tetra-nucleotide repeats.

结果表明，从绵羊皮肤组织相关的EST序列中搜索到微卫星209个，含有微卫星的序列196 个，占整个EST 序列数据库的6.3%，其中双碱基重复150个，三碱基重复19个，四碱基重复12个，五碱基重复17个，六碱基重复11个。

A method for the simultaneous determination of berberine, palmatine, jatrorrhizine, magnoflorine and coptisine from Rhizoma Coptidis samples based on the nonaqueous capillary electrophoresis mode has been developed.

建立了一种非水毛细管电泳同时测定黄连饮片生品与炮制品中小檗碱、巴马汀、药根碱、木兰碱和黄连碱含量的方法。

In the second week,patients in respiratory alkalosis combined metabolic acidosis or respiratory alkalosis mixed acid-base disturbance,should be careful of the sharp type occur and treat it in time.When metabolic alkalosis occur or remove the block of the respiraˉtory,respiratory alkalosis disappear,indicate the disease recover.When some medicine involve acid balance is used must pay attention to the acid-base disorders,adjust it in time,when respirator is used in the treatment,be careful of the adˉjustment to the respiratory alkalosis and must control the pressure level rational.

患者病程2周内出现呼碱代酸或呼碱型三重酸碱失衡，应高度警惕发展为极重型的可能，积极加强治疗；出现单纯的代碱或排除气道阻塞、呼吸肌疲劳及机械通气因素造成的呼酸，以及呼碱消失，可能预示着病情开始恢复；应用某些药物时要随时注意酸碱失衡，及时调整或纠正；应用机械通气要着重对呼碱因素进行治疗，合理调整压力水平。

Objective:An ionpair RPHPLC method was established to determine the amounts of lotusine, liensinine, isoliensinine and neferine in Plumula Nelumbinisimultaneously.

目的：建立同时测定莲子心中四种异喹啉生物碱莲心季铵碱、莲心碱、异莲心碱、甲基莲心碱含量的离子对反相高效液相色谱法。

Ethoxyberberine and 9-isopropanoxyberberine bearing with different sterically hindered groups were synthesized from beberrubine material.

以小檗碱为原料，合成了小檗碱C-9位不同空间位阻取代的9-乙氧基小檗碱、9-异丙氧基小檗碱，通过MS、1H NMR确认其结构。

Results Brucine and its liposome had significant inhibiting effect on transplanted tumor growth in mice. Inhibitory rates of brucine on Heps were 35.05% [1.61mg/], 43.70% [3.23mg/], and 46.09% [6.46mg/], while of its liposome on Reps was 45.41% 1.61mg/1 and 58.19% [3.23 mg/, respectively. The inhibitory rate of brucine on S180 was 37.59% [1.61 mg/], 36.13% 3.23mg/, and 38.87% [6.46 mg/, while of its liposome on S180 was 53.00% [1.61mg/] and 48.37% [3.23mg/], respectively. However, both of them had no distinct effect on prolonging the life of mice with transplanted tumor.

结果 马钱子碱及其脂质体能有效抑制实体瘤模型荷瘤小鼠体内肿瘤生长，1.61、3.23、6.46mg/马钱子碱对Heps小鼠的抑瘤率分别为35.05%、43.70%和46.09%，而1.61、3.23mg/马钱子碱脂质体的抑瘤率分别为45.41%和58.19%；1.61%、3.23、6.46mg/马钱子碱对S180小鼠的抑瘤率分别为37.59%、36.13%和35.57%，而1.61和3.23mg/马钱子碱脂质体的抑瘤率分别为53.00%和48.37%，明显高于马钱子碱；但两者对腹水瘤模型荷瘤小鼠的生存时间均无延长作用。

As caustic solution prewashing of FCC gasoline discharges lots of lye, it is nec essary to improve the process. The solid base pretreating of FCC gasoline wil l completely avoid this problem.

用苛性碱液对汽油进行预碱洗时易产生大量废碱液，为彻底消除废液提出了用固体碱预处理代替苛性碱液预碱洗的方法。

If you are committed to an education career, then the Master of Arts in Education from Ashford University is for you.

如果你是致力于教育事业，那么，艺术教育硕士学位阿什福德大学是给你的。

objective:to explore the expression of survivin protein and its mrna in rectal cancer and their clinic significance.

目的：研究凋亡抑制因子survivin蛋白及其mrna在直肠癌组织中的表达及其与临床病理关系。