碱测定法

The technique of refining mucopolysaccharide from Dasyatis akajei cartilage was studied and the content of mucopolysaccharide was measured.

本文探索了利用碱浸提和酶法去蛋白从赤魟软骨中提取分离纯化粘多糖的技术，并对多糖进行了含量测定。

The technique of extracting and refining mucopolysaccharide from Dasyatis akajei cartilage obtained was studied and the content of mucopolysaccharide was measured.

探索利用碱浸提和酶法去蛋白从赤魟软骨中提取分离纯化黏多糖的技术，并对该多糖进行了含量测定。

The contents of exceedingly weak diabasic alkalies such as sodium DL Hydroxy Succinic acid , sodium citric acid and sodium adipic acid were detected by using the standard curve method.

用标准曲线法测定了极弱二元碱：苹果酸钠、酒石酸钠、柠檬酸钠的含量。

Objective: To develop a RP-HPLC method for simultaneous determination of three active compounds, dictamnine, obacunone and fraxinellone in root bark of Dictamnus dasycarpus and supply a reference for the establishment of the quality standard of D.

中文摘要：目的：建立同时测定白鲜皮中白鲜碱、黄柏酮和梣酮含量的反相高效液相色谱法，为中药白鲜皮标准的制定提供依据。

A HPLC method was established for the determination of gentiopicrin and matrine in Chushi injection.

建立了HPLC法测定除湿注射液中龙胆苦苷和苦参碱的含量。

Lycorine, lycoramine and galanthamine in Lycoris radiata were microwave-assisted extracted and determined by HPLC using triethylamine aqueous solution (pH9.00)-acetonitrile-methanol as the mobile phase.

采用微波辅助提取法提取石蒜中的石蒜碱、力可拉敏和加兰他敏生物碱，建立了微波辅助提取／HPLC测定石蒜中这3种生物碱的分析方法。

Ostrea cucullata was exposed to waterˉsoluble fraction of0 # diesel in laboratory condition,and the DNA damage in the digestive gland and gill was measured by alkaline unwinding assay .

5mg/L、中(2mg/L)和高(5mg/L)3种浓度0#柴油水溶性成分的海水中，利用碱解旋法测定消化腺和鳃DNA损伤的程度。

Delayed Extraction Matrix Assisted Laser Desorption/Ionization Mass Spectrometrywas used to high resolution analysis of mix based DNA samples,using the binary matrices 3 HPA(3 Hydroxy 2 Picolinic Acid)/PAand substrate nitrocellose film.

本文使用延迟引出基体辅助激光解吸/电离质谱( DE- MALDI- MS)法，以3-羟吡啶甲酸和吡啶甲酸混合液为二元基体，硝化纤维膜为基底，对混合碱基组成的 DNA样品进行高分辨率测定，获得满意的结果：DNA2 0 - mer分子离子峰分辩率为 8769，DNA 36- mer为 1 650 ，DNA 54- mer为 843，前二者的组成分子离子峰各同位素峰清晰可辩。

One integrative transformant was obtained through PCR and phenotype identification, designated as A.oryzae ONL1. The 7-day fermentation broth of A.oryzae ONL1 could hydrolyze tributyrin verified by forming transparent cleared zone on tributyrin plate, and the enzyme activity reached 2.5 U/mL. The SDS-PAGE electrophoretic map of the fermentation broth demonstrated that there was a typical RML band at 32.5 kDa.

其培养7天的培养液上清在以三丁酸甘油酯为底物的平板上形成清晰的水解透明圈，碱滴定法酶活测定表明培养液酶活可达2.5 U/mL，培养液上清的SDS-PAGE图谱在32.5 kDa处有RML的特征条带。

The results indicate that the method is simple and rapid for the determination of strong polar trigonelline in Trigonella foenum-graecum L. Furthermore, it significantly reduces the equilibration time compared with ion-pair liquid chromatography recorded in the Pharmacopoeia of China. This new method can be used as a valid method for the quality control of Trigonella foenum-graecum L.

结果表明所建方法分离效果好、快速简易，可以弥补中国药典中离子对色谱法平衡时间过长的缺陷，适用于胡芦巴药材中强极性胡芦巴碱的测定，为胡芦巴的质量控制提供了有效的方法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。