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The cells cultured for 12 days in cultureplates and coated with VN showed positive ALP expression and calcifying nodules.

用以VN包被的培养板培养12 d,碱性磷酸酶染色呈阳性,硝酸银染色显示有钙结节形成。

Alkaline removal is especially effective with etch-resist inks which contain one or more acid metal chelating groups.

碱性去除特别有效的蚀刻,抗拒油墨含有一个或多个酸金属螯合组。

Novel shell cross-linked micelles with pH-responsive PDEA cores and thermo-sensitive PNIPAM coronas in aqueous solution were then fabricated by cross-linking the inner PDMA shells of the PDEA-core micelles.

在碱性条件下,该三嵌段聚合物自组装形成以PDEA为核、PDMA为内壳和PNIPAM为外壳的三层洋葱状胶束。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料,经过过滤去除菌体,上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸,聚唾液酸沉淀用去离子水溶解,再用过滤去除变性蛋白质,滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后,再用过量乙醇沉淀出聚唾液酸,聚唾液酸沉淀用超纯水溶解,用层析柱进行分级纯化,收集洗脱液经过透析,冷冻干燥得聚唾液酸产品。

The rhOP-1 was renatured by dialyzing and reducing the concentration of urea after purification by chromatography. NIH3T3 cells proliferation was assayed with improved MTT method and the activity of ALP was analyzed with PNPP method. At the same time, its activity was analyzed by implanting rhOP-1 into thigh muscles of mouses in vivo.

采用改良MTT法检测rhOP-1对NIH3T3细胞增殖和对硝基苯酚法检测rhOP-1对细胞碱性磷酸酶活性的影响,同时在体内通过小鼠肌袋埋植实验测定rhOP-1活性。

The present invention is particularly applicable but not limited to the treatment of alkaline and acidic non-cyanide zinc electrodeposits to impart improved corrosion resistance and a decorative appearance to the treated substrate.

本发明特别适用但不限于酸性和碱性治疗非氰化锌镀层至传授提高耐蚀、装饰物外观的待遇。

It effectively penetrates and emulsifies oil, grease, dirt to restore the natural luster of all washable surfaces with efficient cleaning performance and leaves pleasant scent after application.

本产品为碱性清洁剂,对油污乳化能力特强,适用于各种防水表面的除油、去渍和除臭等作用,具有良好的清洁用途,用后留有清新香味。

They eat only vegan alkaline forming foods, if they do eat, and perform enemas.

他们吃的是碱性成vegan食品,如果他们吃饭、表演灌肠。

In these fresh running streams, the flamingos drink, having dined in the caustic waters of the lake.

在这些新鲜激流中,火鹤喝水,在湖泊的碱性水中进食。

In view of the paraquat in the gastrointestinal absorption rate of only 5% to 15%, and in acidic and neutral environment of stability, can be hydrolyzed in alkaline solution.

鉴于百草枯在胃肠道的吸收率仅为5%~15%,且在酸性及中性环境中稳定,可在碱性溶液中水解。

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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。

If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。