碱性反应

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Methods To determine the serum PCT of 35 patients with bacteriosis and 15 patients with nonbacterial disease by gold immunochromatographic assay,and determine C-reactive protein and alkaline phosphatase of neutrophilic granulocyte in corresponding period.

方法采用半定量的胶体金免疫结合法，测定35例细菌感染性疾病住院患者及15例非细菌感染性疾病住院患者的PCT，并同期测定患者C-反应蛋白与中性粒细胞碱性磷酸酶。

Basophilic. calcium deposits within the dermis that are variable in size. often surrounded by a foreign body type of granulomatous reaction.

嗜碱性。钙质沈积物存在于不同大小的真皮，这些真皮经常被俱肉芽瘤反应的外来体所包围。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

Then VIII reacted with hydrochloric acid and bromoethlane respectively to get tertiary ammonium chloride and quaternary ammonium bromide cationic surfactants, and the properties of cations were detected by the method of bromophenol blue.

然后再与盐酸和溴乙烷反应，合成铵盐和季铵盐型含氟阳离子表面活性剂，利用酸、碱性溴酚兰实验和铁氰化钾法分别对表面活性剂进行了定性和定量分析。

Resonance light scattering spectrum of cadmium- phenanthorline- bromophenol blue system has been studied. The effects of interference substances and optimization of procedure have been investigated. In the presence of Tris-HCl(pH=9.60),Cd2+ reacts with Phen to form the chelate cation, which further reacts with acid dye of BPB to form an ion-associated complex. Then, the complex molecules are accumulated to particles by virtue of hydrophobic forces and other molecular forces. This results in significant enhancement of RLS intensity, and the enhanced intensity of RLS at 638nm is proportional to the concentration of Cd~(2+).

研究了镉-邻菲罗啉-溴酚蓝体系在碱性条件下的共振光散射光谱特征，考察了体系的影响因素和最佳反应条件，研究表明，在pH=9.60的Tris-HCl介质中，镉与邻菲罗啉形成络阳离子，再与酸性染料溴酚蓝形成离子缔合配合物，在疏水力等分子间作用力下，缔合物分子聚集形成缔合微粒，溶液的共振散射光显著增强；在638nm处RLS增强程度与镉浓度成线性关系，线性范围为0-3.20mg/L，检出限为4.50ug/L。

The activities of succinate dehydrogenase in hepatocytes, alkaline phosphatase and thiamine pyrophosphatase on the wall of bile canaliculi became obviously less.Both the activities and localizations of TPPase had changed. Some TPPase from the damaged lysosome-like vesicles and Golgi saccules of hepatocytes discharged into the cytoplasm. TPPase reaction product in some bile canaliculi decreased.

酶细胞化学技术显示肝细胞线粒体琥珀酸脱氢酶和毛细胆管壁上的碱性磷酸酶活性明显减弱或消失，焦磷酸硫胺素酶的活性及定位也发生了改变， TPPase从损伤的高尔基复合体及溶酶体样小泡漏出进入胞浆，部分毛细胆管壁上的TPPase反应物减少。

Five polychlorobiphenyl monomers were synthesized by addition-elimination reaction between chloric aniline and benzene in the presence of alkaline.

在碱性的条件下，以氯代苯胺为重氮化试剂，与苯、氯苯等发生加成反应，合成了5种多氯联苯单体，其结构经1H NMR和IR表征。

After derivatization with 9-fluolenylmeghyl chloroformate at 40℃ in alkalescent environment (pH=8.0), taurine in samples was separated and quantified by HPLC.

在硼酸盐缓冲溶液存在的弱碱性(pH=8.0)环境中样品中的牛磺酸与9-氯甲酸芴甲酯在40℃水浴中反应生成衍生化产物，用反相高效液相色谱法检测定量。

The preparation method comprises removing benzyl of the compound IV0 in alkali condition to obtain the compound (V, reacting the compound with benzyl chloroformate to obtain the compound, wherein the compounds can be used to prepare 5-substituted-hydride indolizine natural alkaloids.

本发明所提供的制备方法是将化合物在碱性条件下去苄基得到的化合物，将化合物与氯甲酸苄酯反应，生成的化合物。和化合物可以用于制备5-取代-氢化中氮茚天然生物碱。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。