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High performance liquid chromatography was used to detect concentration of BP in the air of working sites and level of urinary 1-hydroxypyrene (1-OH-Py) of workers. Contents of acetylcholine in blood filtrate and activities of erythrocyte acetylcholinesterase were determined by Alky hydroxylamine chromatometry and DTNB methods, respectively.

用高效液相色谱法测定各组工作场所空气中BP的浓度,并对工人尿中1-羟基芘(1-OH-Py)的水平进行检测;分别用碱性羟胺比色法和二硫代二硝基苯甲酸法测定全血滤液中乙酰胆碱的含量和红细胞乙酰胆碱酯酶的活性。

Methods: from prepation of crude drug as start, study the technology of extract and defat with L9 (3~4) orthogonal test. With EtOH extraction and water sed, alkali washing, EtOAC extraction as main methods of eliminate dopant. Finally with alky AlzO3 chromatographic column, rich and gain anticancer active site. The maximum response was achieved at a 40 min extracting time with 10 times volume 90% EtOH added to the sample for the first time and 30 min for the second time. Adding 10 times water will produce precipitation; Place the precipitation into 6 times petroleum benzin to de-fat at 60℃.

云南红豆杉中抗癌活性物制备工艺研究方法:利用正交设计试验L9(3~4)确定原料药提取工艺为:10倍量90%乙醇提取两次,第一次40分钟,第二次30分钟;醇提液用10倍量水沉;沉淀加6倍量石油醚40℃脱脂;用1mol/L的NaOH溶液碱洗,除去酸性杂质;以碱性氧化铝柱,以环己烷/丙酮为洗脱溶剂,在环己烷-丙酮(6:4)段富集到所需活性部位,洗脱剂用量为3VB。

The effects of above-mentionedconditions on alkylene reaction are studied to obtain the optimum reactive conditionsby orthogonal experiments having seven factors and three levels. GCLE is chosen as material to prepare 7-phenyl acetamideyl vinyl cephalkyl acidp-methoxy benyl eatsr in the experiment. The process is as follow: GCLE reacts withiodide in solvent, and produce 3-iodid, then reacts with PPh_3 to produce Witting agent,and reacts with HCHO to produce 7-phenyl acetamideyl vinyl cephalkyl acid p-methoxy benyl eatsr in alkaline conditions.

试验选用GCLE作为原料制备7-苯乙酰氨基-3-乙烯基头孢烷酸对甲氧苄酯,反应过程如下:GCLE与碘化物、PPh3在溶剂中反应生成3位膦盐,在碱性条件下生成Wittig试剂再与HCHO反应生成7-苯乙酰氨基-3-乙烯基头孢烷酸对甲氧苄酯。

The catalytic effect of basic lithium phosphate on the preparation of allyl alcohol by isomerization of propylene oxide was studied in this dissertation.

本论文主要研究了碱性磷酸锂在环氧丙烷异构化制烯丙醇反应中的催化作用。

The isomerization reaction of propylene oxide to produce allyl alcohol catalyzed by basic lithium phosphate was studied.

研究了环氧丙烷在碱性磷酸锂催化作用下异构化生成烯丙醇反应过程,考察催化剂的制备条件,异构化温度对催化性能的影响,以及催化反应机理和动力学特性。

Based on the work of the first part, we studied the possibility of allyl as a protection group in the synthesis of oligonucleotides consisting in some unstable nucleotide analogues, such as nueleoside carbonate and sulfite.

在寡核苷酸的类似物中,碳酸酯及亚硫酸酯在碱性条件下的不稳定性使其在合成上有一定的困难,这限制了对它们的性质作进一步的研究。

Objective:To investigate the effects of mouse recombinantPeriostin on proliferation and ALP activity of periodontal ligament cells.

目的:研究小鼠重组Periostin对牙周膜细胞增殖及碱性磷酸酶活性的影响。

MAIN OUTCOME MEASURES: The proliferation rate and the ALP activity of osteoblasts.

主要观察指标:成骨细胞的增殖率和碱性磷酸酶活性。

Higher than normal levels of ALP may indicate liver damage or disease.

高於正常水平的碱性磷酸酶可能表明肝损伤或疾病。

MAIN OUTCOME MEASURES: The ratio of absorbance value between ALP and total protein.

主要观察指标:碱性磷酸酶与总蛋白的吸光度值。

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