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Objective: The purposes of this project include: to explore the activated basophils expression ratio of acute, chronic urticaria patients and healthy controls peripheral blood.

目的:分析急慢性荨麻疹患者外周血中活化的嗜碱性粒细胞的数量,以及在受到不同变应原刺激之后嗜碱性粒细胞的活化程度。

Objective To investigate the distribution of basophils in airway walls of fatal asthmatics, nonfatal asthmatics and non-asthmatics by using a novel monoclonal antibody.

目的 采用一种新的针对嗜碱性粒细胞的单克隆抗体,对不同程度支气管哮喘患者支气管肺组织进行免疫组织化学检测,以明确嗜碱性粒细胞在不同组织结构中的分布及其临床意义。

The extracts of water and alcohol were separated by ion exchange resin. The eluates of neutral solution were the most effective against hydroxyl radicals and the highest rate of inhibiting hydroxyl radicals was 81%. The eluates of basic solution were also effective, the inhibit rate of which was 60%.

将发酵灵芝粉的水和醇提取物上离子交换树脂柱,依次用酸性、中性、碱性淋洗液洗脱分组,发现中性洗脱部分对羟基自由基抑制作用最强,抑制率最高可达81%;碱性洗脱部分亦具有抑制羟基自由基的效果;酸性洗脱部分抑制羟基自由基的作用不强,且占很少比例。

An alkaline protease Alcalase AF 2.4 L was used to hydrolyze corn gluten meal and study the effects of pH and enzyme concentration on protein conversion. The optimum technological conditions were obtained in this test as follow: hydrolyzing temperature 55℃,addition of 2 ml alkaline protease(90 947 U/ml),pH=9,concentration of substrate 0.1g/ml,hydrolysis time 1 h.

研究了碱性蛋白酶Alcalase AF 2.4L水解玉米蛋白时,pH值和酶浓度对蛋白质转化率的影响,得到了实验范围内的最佳工艺条件:水解温度55℃,碱性蛋白酶加入量2ml(90 947U/ml),pH值9,底物浓度0.1g/ml,水解时间1h。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论:成骨细胞呈梭形,多突起;细胞核呈卵圆形,偏于一侧;胞体大,胞浆丰富;碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒,某些部位染成黑色,定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞;细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

Their activities, heat-proof, acid-proof and alkaline-proof of the two enzymes were inhibited with increasing TBT concentrations.The effect of TBT on the phagocytic activity of oyster haemocytes was studied in vitro and in vivo.

从未受污染的牡蛎个体中分离的碱性磷酸酶和超氧化物歧化酶的活性、耐热性、耐酸碱性均随TBT浓度的增大而降低。

Pulp reactions were obvious during 3 days after lased. Alkaline phosphatase examination: After fixed, decalcified and selected, all experimented teeth were stained with the Ca-Co method in order to examine the alkaline phosphatase activity in dental pulp cells.

碱性磷酸酶的检测:实验牙在丙酮中固定1 d,再置入复合酸中脱钙,冰冻切片后,进行钙-钴法染色以显示牙髓细胞中碱性磷酸酶活性,根据米德冷图象分析系统测定切片的灰度值,进行统计学分析。

In this article, the effect of different water pH (pH 5, 6, 7, 8, 9)on the secretion of nacre of freshwater pearl mussel Hyriopsis cumingii were studied with histochemical methods and transmission electron microscopy.

结果表明:在中性水环境中,贝体能积极地从外界水环境中吸收钙,并能旺盛地合成和分泌贝壳珍珠层及珍珠有机基质前体物质;持续的酸性水环境导致贝体的钙严重丢失,并引起珍珠质分泌细胞对有机基质前体物质的合成和分泌能力减弱;持续的碱性水环境虽能导致贝体对钙的积累,但珍珠质分泌细胞合成和分泌珍珠质有机基质前体物质的能力减弱;水环境的酸性或碱性程度越高,对珍珠质分泌细胞的合成和分泌活动的影响越大。

The C-terminal domain, which is centered with a triple-stranded antiparallel β sheet, consists of four helices and three β strands. Two monomers further assemble into a functional dimer. Compared with other manganese dismutases, GP-MnSOD shows two significant characters.

与其它MnSOD比较,GP-MnSOD具有两个显著的特征:(1)底物通道入口增加了碱性残基Lys38;(2)底物通道以外的蛋白表面覆盖的酸性残基数量大大高于碱性残基的数量。

The analysis of polymer structure indicates that the non-crystalline phase is the majority of the agglomerate structure and the crystalline phase is the minority, the highest crystal value only reaches the level of 0.1646;There is a definite interactionadditives and PVA, which not only reduces the crystal value of ASPE to make amorphous areas enlarge but also improve the ability of segmental motion by destroying the well-regulated and orderly character of polymer chain, meanwhile restricts the mobility of potassium cation and enhances the anionic transference number of hydroxide ions, all these improve the ionic conductivity;The ionic conductivity occurs essentially within the amorphous phase of the ASPE;The structure of ASPE doesnt change in the range of our test temperature, this means, the temperature only takes effect on degradation of complex in certain compositions, ionic mobility and segmental motions of the polymer chain.

聚合物结构分析表明:碱性固体聚合物电解质中凝聚态结构以非晶态为主,仅有少量的晶态,结晶度最大为0.1646;添加的各组分与PVA之间都有一定的相互作用,这种作用不仅破坏了聚合物链段的规整性,能够降低聚合物的结晶度,增大无定形区域,提高链段运动的能力,同时这种作用限制K~+离子的运动,提高OH~-的迁移率,从而提高离子电导率;碱性聚合物电解质的离子传导主要发生在无定形区;在本实验测试离子电导率温度范围内,聚合物内部的结构不会发生变化,即温度只会影响电解质内部络合物解离程度和载流子迁移以及聚合物链段运动的情况。

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