碱

The results showed as follows: 1 Extraction of gelsemium total alkaloids. Total alkaloids were extracted with 95 per cent ethanol. The extract rate was 0.519 per cent and the extract purity were 88.02 per cent and 87.38 per cent; Total alkaloids were extracted with chloroform .The extract rate was 0.416 per cent and the extract purity were 89.02 per cent and 94.52 per cent. 2 Isolation of gelsemium total alkaloids with TLC. Total alkaloids contained five or more alkaloids. The optimal developing agent was chloroform :methanol :ethyl acetate(9:l:0.3),the optimal eluent was methylene chloride:methanol(92:8). 3 Isolation of total alkaloids with column chromatography. Alkaloid A and alkaloid B were obtained, alkaloid B was identified as koumine.

研究结果如下：1、钩吻总碱的提取：用95％的乙醇提取钩吻生物碱提取率为0.519％，其提取物纯度为88.02％、87.35％；用氯仿提取钩吻生物碱提取率为0.416％，其提取物纯度为89.02％、94.52％。2、钩吻总碱的薄层层析分离：总碱中含有5种或5种已上的生物碱；氯仿：甲醇：乙酸乙酯(9：1：0.3)是最佳薄层层析的展开剂；二氯甲烷：甲醇(92：8)是柱层析的最佳洗脱剂。3、钩吻总碱的柱层析分离：共得到两种钩吻生物碱单体成分碱A和碱B，碱B为钩吻素子，碱A可能是钩吻素甲。4、钩吻总碱的急性毒性试验：钩吻总碱小鼠腹腔注射的半数致死量LD_(50是：7.38mg／kg，95％的可信限范围为：6.67mg／kg～7.94mg／kg。

Macerate 1.0g of the powdered sample with 20.0 ml of chloroform plus 0.3ml of 25％ NH_4 OH in a glassstoppered flask overnight.

本文报道用不含粘合剂的氧化铝薄层，以二甲苯-丙酮-无水乙醇-二乙胺(50：40：10：0.6)为展开剂分离茄科植物中的莨菪碱、东莨菪碱、山莨菪碱、樟柳碱及红古豆碱等五种莨菪烷生物碱，用改良Dragendorff试剂-Wagner试剂(1：1)显色，定量收集莨菪碱、东莨菪碱及山莨菪碱的斑点，莨菪碱和东莨菪碱用氨甲醇洗脱，蒸干洗脱液后分别用pH 5.6及pH 4.5的溴甲酚绿溶液作比色测定；山莨菪碱用4％醋酐氯仿洗脱，蒸干洗脱液后用pH 5.6的溴甲酚绿溶液作比色测定。

Flushes is cool when should take down the jewelry, meets 碱 a kind of product by against jewelry, after specially contains the chlorine bleaching agent and the silver contact, can have the chlorination to respond, causes the silver personal ornament rapid nigrescence.

冲凉时应取下首饰，以防首饰遇到碱类产品，特别是含氯的漂白剂和银接触后，会起氯化反应，使银饰迅速变黑。

The constituents of bulb of F. puqiensis were studied. Five steroid alkaloids were isolated. The plane structure and stereoscopic configuration of 4 compounds were elucidated. They are named as puqienine A (FP-33), puqienine B (FP-3), N-demethyl-puqietinone (〓 FP-34) and puqietinone (〓, FP-9). Puqienine A and N-demethyl-puqietinone are new compounds. Puqienine B is as same as FPQ-3 elucidated by Prof.

对F.puqiensis鳞茎化学成份进行了研究，分得5个甾体类生物碱成分，并采用 UV、IR、EI-MS、1D-NMR，HHCOSY、CHCOSY、HMBC和X-RAY等光谱技术和理化常数测定，鉴定出4个化合物的平面结构和立体构型，分别为蒲圻贝母碱甲（puqienineA，FP-33）、蒲圻贝母碱乙（puqienineB，FP-3）、N-去甲蒲贝酮碱（N-demethyl-puqietinone，FP-34）和蒲贝酮碱（puqietinone，FP-9），其中蒲圻贝母碱甲和N-去甲蒲贝酮碱为新化合物。

This dissertation involves the study of the growth dynamics of colloidal particles under unequilibrium and irreversibility condition. Firstly, it is of interest to investigate whether the ideal diffusion or reaction-limited aggregation universal behavior can be observed for the initially polydisperse practical system of basic yttrium carbonate as well. The fractal structure of basic yttrium carbonate aggregate cluster was observed using transmission eletron microscopy. The aggregation kinetics of basic yttrium carbonate colloids was studed using dynamic 1ight-scattering techniques. At 1.00mol/l NaCl solution the fast diffusion-limited aggregation behavior was observed with an increase in the aggregate size over time described by a power law. The aggregates are characterized by a fractal dimension D=1.82. The disordered and ramified cluster is consisted of primary colloidal particles and tenuous and more open. These are in good agreement with those observed for other colloidal system such as colloidal gold (1.86), silica (1.85) and polystyrene latex (1.82) in diffusion-limited aggregation from the static and dynamic light scattering by Lin. Therefore ideal diffusion-limited aggregation behavior can be observed in practical polydisperse system as well. Smoluchwski and fractal theories were used to obtain the aggregation rate constant for initially polydisperse system for the first time.

本论文研究胶粒的非平衡不可逆聚集动态学，分为两个部分，第一部分是通过实验研究一个新的初始多分散的碱式碳酸钇胶粒实际体系中，聚集标度性质的普适性，即使用透射电子显微技术观测聚集粒子簇的分形结构，使用动态激光光散射研究碱式碳酸钇胶粒的聚集动力学，胶粒带电而产生静电排斥作用使得碱式碳酸钇胶粒处于稳定状态，当加入电解质NaCl溶液时，碱式碳酸钇胶粒间的Debye-Hückel屏蔽距离缩短，从而降低静电排斥能垒，引起胶粒发生不可逆聚集，加入电解质NaCl的浓度为1.00mol/l时，碱式碳酸钇胶粒进行快速聚集，经历扩散控制聚集机理，形成结构疏松、更为开放的分形聚集粒子簇，其分形维数为1.82，碱式碳酸钇胶粒聚集增长动力学遵循幂函数增长规律，与Lin等使用静态激光光散射和动态激光光散射测量硅胶(1.85)、金胶(1.86)和聚苯乙烯胶乳(1.82)在扩散控制聚集状态形成聚集粒子簇的分形维数值以及粒子簇聚集增长的动力学规律相一致，表明胶粒聚集标度性质的普适性质在初始多分散的实际体系中也是存在的，首次由动态激光光散射的实验数据得到初始多分散胶粒分形粒子簇的聚集速率常数，并且其值与Smoluchowski聚集速率理论预期相符。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Result: Eight compounds were isolated and identified as matrine,sophoridine,sophocarpine,sophoramine,oxymatrine,oxysophocarpine,aloperine and sparteine.

结果：分离鉴定出8个化合物：苦参碱、槐定碱(sophoridine，Ⅱ)、槐果碱(sophocarpine，Ⅲ)、槐胺碱(sophoramine，Ⅳ)、氧化苦参碱(oxymatrine，Ⅴ)、氧化槐果碱(oxysophocarpine，Ⅵ)、苦豆碱(aloperine，Ⅶ)和鹰爪豆碱(sparteine，Ⅷ)。

Till today, we have extracted and purified alkaloids from from Macleaya Cordata, Chelidonium Majus L., Tripterygium Wilfordii Hook f., Sedum Sarmentosum Bunge, and obtained pure sanguinarine, ethoxysanguinarine, oxysanguinarine, chelidonine, chelerythrine, ethoxychelerythrine, chelilutine, chelirubine, protopine,α-allocryptopine,β-allocryptopine, coptisine, berberine, corysamine, bocconine; triptolide, tripdiolide, triptonide, wilforddine; N-methylisopelletierine, dihydro-N-methylisopelletierine, sarmentosin etc.

我们对博落回、白屈菜、雷公藤、垂盘草等天然植物进行了大量的提取和分离研究；并分离出了血根碱、乙氧基血根碱、氧化血根碱、白屈菜碱、白屈菜红碱、乙氧基白屈菜红碱、白屈菜黄碱、白屈菜玉红碱、原阿片碱、α-别隐品碱、β-别隐品碱、黄连碱、小檗碱、沙明碱、博落回碱，雷公藤内酯醇、雷公藤内酯二醇、雷公藤内酯酮、雷公藤碱，N-甲基异石榴皮碱、二氢- N-甲基异石榴皮碱、垂盘草甙等单体。

In order to meet the market development, our factory introduced a 55% focus on content protopine, protopine content 60%, 65% content protopine, and 70% content protopine, 75% and 80% and other low-grade protopine, low-grade films alkali, low-level protopine, and 90% content of 92% and other non-GB Product in order to adapt to different manufacturers and the general needs of distributors, products are exported to Shandong, Jiangsu, Zhejiang, Anhui, Guangzhou and other places, by our customers trust and.

为适应市场发展我厂重点推出了55%含量片碱，60%含量片碱，65%含量片碱，和70%含量片碱，75%和80%等各种低度片碱，低度片碱，低含量片碱，以及90%含量92%等非国标产品，以适应不同厂家和广大经销商的需求，产品远销山东，江苏，浙江，安徽，广州等地，深受客户的信赖和欢迎。

rutaecarpine pharmacological effects and its related mechanism: rutaecarpine the heart positive inotropic, positive changes in the role and the heart protective effect, which with its promotion of calcitonin gene-related peptide release; rutaecarpine alkali have vasodilative and hypotensive effect, its mechanism mainly related to endothelial cells Ca2 +-NO-cGMP pathways and calcitonin gene-related peptide release; rutaecarpine to relax the anal sphincter, gastrointestinal motility inhibition of stress ulcer and ulcerative colitis have therapeutic effect; rutaecarpine on cerebral ischemia-reperfusion injury has a protective effect, which instead of increasing the concentration of calcitonin gene-related peptide-related; rutaecarpine inhibits platelet aggregation and arterial thrombosis; In addition, rutaecarpine Alkali also anti-inflammatory and analgesic, anti-tumor and other pharmacological effects.

综述了吴茱萸次碱的药理作用及其相关机制：吴茱萸次碱对心脏有正性肌力、正性变时作用和心脏保护作用，这与其促进降钙素基因相关肽释放有关；吴茱萸次碱有扩血管和降压作用，其机制主要涉及内皮细胞Ca2+-NO-cGMP途径和降钙素基因相关肽的释放；吴茱萸次碱能松弛肛门内括约肌，抑制胃肠运动，对应激性溃疡和溃疡性结肠炎也有治疗作用；吴茱萸次碱对脑缺血再灌注损伤有保护作用，这与其增加降钙素基因相关肽浓度有关；吴茱萸次碱能抑制血小板聚集和动脉血栓形成；此外，吴茱萸次碱还有抗炎镇痛、抗肿瘤等药理作用。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。