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Add 0.1% Dimethyl Sulfate to cell suspensions to methylate free G residues.

调整浓度后的细胞悬液中加入0.1%的硫酸二甲酯(DimethylSulsate,DMS),进行甲基化处理。

Silicon phthalocyanine dihydroxide was prepared from silicon phthalocyanine dichloride through two methods. The first was alkali treating that meaned silicon phthalocyanine dihydroxide was treated in the ethanol solution containing sodium methylate .The second was acid treating meaned precipitating its solution in concentrated sulfuric acid into ice water. The product yield of the second method was 77.0%, which was higher than the method of alkali treating.

此外将合成的二氯酞菁硅采用两种方法转化为二羟基酞菁硅,第一种方法是碱处理法即将二氯酞菁硅在甲醇钠的乙醇溶液中回流得到,最高收率为53%第二种方法是酸处理法即采用二氯酞菁硅的硫酸溶液在冰水析出的方法转化为二羟基酞菁硅,其中酸溶析出法收率为77.0%。

The method on trans fatty acids analysis in food is very important. The effects of methylate sodium, potassium hydroxide, sulfuric acid and boron trifluoride in methanol and their different concentration and dosage on TFA analysis were studied. The chromatographic condition and the influence of time and temperature on the esterifation of TFA using boron trifluoride in methanol were optimized. A method for analyzing TFA were established by GC based on boron trifluoride in methanol and RT-2560(100m×0.25mm×0.2μm) capillary column.

研究了甲醇钠-甲醇、氢氧化钾-甲醇、浓硫酸-甲醇和三氟化硼-甲醇4种甲酯化试剂的浓度、用量及甲酯化时间和温度对反式脂肪酸检测的影响,优化了色谱条件,建立了以三氟化硼-甲醇法甲酯化,RT-2560(100m×0.25mm×0.2μm)毛细管柱为基础的反式脂肪酸气相色谱检测方法,C18:1-9 t, C18:1-11 t, C18:2-9 t, 12 t和C22:1-13 t 4种反式脂肪酸的最小检出浓度在0.774.08 mg/kg之间。4种反式脂肪酸不同添标浓度回收率为98.44%101.23%,重复测定的相对标准差在0.22%1.31%之间,有较好的准确度和重现性。

The optimal conditions were 30 ℃,28.26 lx,2 min,λ40nm, n (AgNO\-3)∶ n=2∶1,dosage of metol 0.5 g/L and dosage of hydroquinone 5 g/L.

适宜条件为 30℃,2 8.2 6lx,2min ,λ 4 0nm ,n(AgNO3 )∶n =2∶1,硫酸对甲胺基苯酚用量为 0 5g/L ,对苯二酚用量为 5g/L。

The CNTs were characterized with the transmission electron microscope and infrared spectroscopy. In addition, the dispersion of the CNTs in water was investigated with the optical microcopy and the viscosity meter. The FTIR spectrum experiments showed that there were many hydroxyl groups and carboxyl groups, on the surface of the acid-treated CNTs.

采用硫酸和硝酸混合酸对催化裂解法制备的多壁碳纳米管进行纯化,然后运用十六烷基三甲基溴化胺对CNTs进行修饰,并利用透射电子显微镜、红外光谱和拉曼光谱表征了CNTs的表面结构;另外,通过数码照相机和乌氏粘度计研究了修饰的CNTs在水中的分散性能。

Objective To explore the mitochondria and microsome of testis cells caused by nickel sulfate in rate.

目的从氧化应激角度探讨硫酸镍(NiSO4)对大鼠睾丸细胞线粒体及微粒体的毒性作用。

Abstract摘要: Objective To explore the mitochondria and microsome of testis cells caused by nickel sulfate in rate.

摘要摘要:目的从氧化应激角度探索硫酸镍(NiSO4)对大鼠睾丸细胞线粒体及微粒体的毒性功能。

The experimental results indicated that accelerated corrosion of plain 12CrMoV and mild steel was caused by SO_3 from decomposition of ferric sulfate.

实验结果表明由于硫酸铁分解产生的SO_3引起了12CrMoV、20~#钢的加速腐蚀。

Said method includes the following steps: firstly, adopting dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, citric acid, ammonium ferric citrate, disodium EDTA, sodium carbonate and minor element solution A5 to prepare culture liquor of nostoc according to a certain ratio; then controlling temp. light intensity and deration condition of the culture liquor to make culture of nostoc; third ste, harvesting nostoc; fourth ste, extracting nostoc polysaccharide; and fifth step, purifying, harvesing and drying nostoc polysaccharide.

首先是配制葛仙米培养液:采用磷酸氢二钾、硫酸镁、氯化钙、柠檬酸、柠檬酸铁铵、乙二胺四乙酸二钠盐、碳酸钠,微量元素溶液A5按比例配制;其次是葛仙米的培养:控制温度、光强、光质、培养液通气条件;第三是葛仙米的收获;第四是葛仙米多糖的提取;第五是葛仙米多糖的纯化、收获与干燥。

The influence of temperature, catalyst and the molar ratio of raw materials on the yield of the product were studied.

分别考察了硫酸氢钠和对甲苯磺酸对该反应的催化性能,确定了各自的最佳合成条件。

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呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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