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Gold nanoparticles was self-assembled onto a platinum electrode to form a negatively charged surface, and cationic dye-thionine was linked by Au—S and Au—N covalent bond and electrostatic adsorption as an electron transfer mediator.

在铂电极上自组装一层纳米金,构建负电荷的界面,然后通过金-硫、金-氮共价键合作用和静电吸附作用自组装一层阳离子电子媒介体硫堇

The electrochemical polymerization of thionine in the aqueous acetic solution has been carried out using cyclic voltammetry.

使用循环伏安法实现了硫堇在酸性溶液中的电化学聚合。

The top is colored with silver stain, and the bottom treated with thionine.

图中上面的切片经过银黄着色,下面的切片则用硫堇加以处理。

Visible and FTIR spectra of polythionine have been presented in this paper. Based on FTIR spectra, a suggestion for mechanism of polymerization of thionine is presented.

测定了聚硫堇的可见光谱和红外光谱,根据红外光谱,提出了硫堇的电化学聚合机理的假设。

It was found that thionine could mediate the electron transfer of HRP with the electrode substrate.

硫堇的表观覆盖度为8.54×10〓mol/cm〓,硫堇与玻碳电极之间的异相电子转移速率常数为0.97s〓。

A coronal section of a whole human brain stained with thionine.

整个人脑的冠部经过硫堇染色。

A new fluotescence quenching method has been developed to determine nitrite in water samples. The method is based on the diazo-reaction between nitrite ion and thionine in dilute suifuric acid medium.

文章研究了在稀硫酸介质中亚硝酸根与硫堇发生的重氮化反应,基于硫堇的荧光强度随亚硝酸根的加入量增加而明显降低的现象,建立了荧光猝灭法测定痕量亚硝酸根的新方法。

The fluorescence emission peak of inclusion complex shifted toward short waves and fluorescence intensities decreased in the presence of DNA. The quenching constant was calculated to be 6.12×10~4L mol~(-1) by Stern-Volmer method. The quenching mechanism was studied. The interaction ofβ-CD-TH inclusion complex and thionine with DNA were compared.

荧光光谱实验的结果是DNA对硫堇包合物的荧光峰有蓝移和猝灭效应,猝灭常数Ksv为6.12×10~4L mol~(-1);探讨了其荧光猝灭机理,比较了硫堇包合物和游离态硫堇与DNA作用程度的大小。

The thionine was successfully modified on pretreated PPCF microelectrode by electrochemical polymerization.

通过电聚合方法在石油沥青基碳纤维微电极表面修饰硫堇,获得了理想的修饰层。

INTERVENTIONS: 4 vessel occlusion(4VO) brain ischemic models in rats stained with thionine staining and GFAP immunohistochemistry staining. were used. Sixty-four rats, of which bilateral vertebral arteries were occluded permanently by electrocautery, were divided into the following 8groups: sham operation group, cerebral ischemic preconditioninggroup, ischemic insult group; BIT group; MTPG + sham operation group;MTPG+BIT group; MTPG+ischemia group and -4C3HPG+BIT coup. All the rats were killed 7 days after the operation or the final ischemic treatment. Cerebral sections were selected and stained with thionine staining and GFAP immunohistochemistry staining.

干预:采用大鼠四血管闭塞全脑缺血模型,应用硫堇染色和胶质纤维酸性蛋白免疫组化法。64只大鼠椎动脉凝闭后分为假手术组、单纯预处理组、单纯缺血组、脑缺血耐受组,MTPG+假手术组、MTPG+脑缺血耐受组、MTPG+缺血组和-4C3HPG+脑缺血耐受组,所有动物均在手术后或末次缺血后7 d处死取材,行脑组织切片,硫堇染色和胶质纤维酸性蛋白免疫组化染色。

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