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硫因

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Isolation, purification and characteristics of D-hydantoinase were carried out. The experiment results showed that the optimal pH and temperature of pure D-hydantoinase were pH 8. 5 and 35℃, respectively. D-hydantoinase activity could be increased significantly by Mn〓 and Fe〓. Substrate specificity of D-hydantoinase on various hydantoin derivatives showed the optimal substrate of D-hydantoinase was 5-phenylhydantoin, but hydantoin, 5-methylthioehylhydantoin, 5-hydroxyphenylhydantoin, 5-methylhydantoin, 5-benzylhydantoin could also be hydrolyzed by D-hydantoinase.

本文还进行了海因酶的分离纯化及性质研究,发现酶的最适反应温度为35℃,最适pH为8.5,金属离子Mn〓和Fe〓能显著提高D-海因酶活力。D-海因酶的特异性实验表明,D-海因酶的最适底物为D,L-苯海因,其次为海因,D,L-对羟基苯海因和D,L-甲硫乙基海因,对D,L-甲基海因和D,L-苯甲基海因也有一定的作用。

In conclution, PFA and PFB prepared from the solid state fermentation of polished adlay and buckwheat have better nutritional, taste quality and physiologically active components, antioxidant properties and anti-inflammation activity.

综合以上结果,薏仁与荞麦经固态发酵制备成高麦角硫因之杏鲍菇薏仁和杏鲍菇荞麦,具有提高营养、呈味特性、生理活性物质含量、抗氧化及抗发炎性质。

Inonotus obliquus is precious medicinal mushroom due to the fact that it contains many physiologically active materials, including polysaccharides, adenosine, ergosterol and ergothioneine.

中文摘要白桦茸为一种非常珍贵的药用真菌,含有许多生理活性物质,包括:多醣体、腺苷、麦角固醇和麦角硫因

Ergothioneine of PFA and PFB at various culture condition, investigated optimal incubation to intent to get hight content of ergothioneine.

在不同发酵条件下生长所得之杏鲍菇薏仁和杏鲍菇荞麦,探讨获得最大麦角硫因含量之最适培养组成。

Results showed that optimal conditions for high content of ergothioneine were moisture content at 40%, temperature at 30℃and polished adlay as medium, cubation 17 days (574.0 mg/kg dw), biomass was 139.0 mg/g dw.

经探讨后,以含水量40%之薏仁於30 ℃下培养17天后之麦角硫因量最高,可达574.0 mg/kg dw,再添加酵母萃取物为氮源可提高麦角硫因到824.8 mg/kg dw,生物质为139.0 mg/g dw;以含水量45%之荞麦於30 ℃下培养17天后之麦角硫因含量最高,可达580.6 mg/kg dw,生物质为163.4 mg/g dw,添加酵母萃取物为氮源,麦角硫因含量可达651.6 mg/kg dw。

With regard to physiologically active components, the contents of ergosterol and ergothioneine of mycelia (2056.4 mg/kg dw and 1514.6 mg/kg dw) were higher than others.

在生理活性方面,麦角固醇含量上以杏鲍菇菌丝体(2056.4 mg/kg dw)>杏鲍菇荞麦(469.2 mg/kg dw)>杏鲍菇薏仁(443.4 mg/kg dw);麦角硫因含量也是以杏鲍菇菌丝体最高(1514.6 mg/kg dw)。

Ergothioneine is a kind of precious amino acid, and it is also a natural antioxidant. It plays an important role to prevent the oxidative damage in organism. Ergothioneine cannot be biosynthesized in humans, only can be absorbed from the diet. In recently the research discovered the high content of ergothioneine in the mushroom. Content of ergothioneine of Pleurotus eryngii is the highest from 28 kinds of mushrooms, therefore the research untilized P. eryngii to make the products of P. eryngii-fermented adlay and P. eryngii-fermented buckwheat which content high ergothioneine. Also, we analyzed their proximate composition, nutritional components, taste and physiologically active components, antioxidant properties and evaluation of anti-imflammatory effect.

中文摘要麦角硫因为一种稀有的胺基酸,也是天然的抗氧化剂,其在生物体内对氧化压力伤害之预防扮演著重要角色,麦角硫因不能在动物体内合成,仅能由食物中摄取,近年来发现在菇类中含有高含量的麦角硫因,从28种食药用菇类中筛选出麦角硫因含量较高的菌种,以杏鲍菇子实体最高(1521.6 mg/g dw),因此本论文以杏鲍菇为媒介,利用固态发酵方式,制备出高麦角硫因之杏鲍菇薏仁和杏鲍菇荞麦,并针对薏仁、杏鲍菇薏仁、荞麦、杏鲍菇荞麦和杏鲍菇菌丝体,进行一般成分、营养、物性、呈味、生理活性物质、抗氧化性质之分析及抗发炎反应之评估。

The addition of yeast extract for nitrogen source can increase content of ergothioneine (824.8 mg/ml dw);Fermentation of Pleurotus eryngii was polished buckwheat as medium, moisture content at 45%, temperature at 30℃and cubation 17 days could get high content of ergothioneine (580.6 mg/kg dw), and biomass was 163.4 mg/g dw, yeast extract as nitrogen source (651.6 mg/kg dw), Fructose as carbon source (721.4 mg/kg dw).

在碳源方面,添加果醣能提高麦角硫因含量为721.4 mg/kg dw。

The results showed that PIG28 encoding protein, ABC transporter, metallothioneine, polyubiquitin 2, plasma membrane H+-ATPase, amino acid permease etc.

同时,得到PIG28编码蛋白、ABC transporter、金属硫因、泛素、plasma membrane H+-ATPase、amino acid permease等寄主与病原菌互作相关蛋白。

Basing on experimental study, the article analyses the process of calcination and desulfuration reaction and builds a mathematical model of calcium-based materials (limestone, shell and their mixture), which will provide theoretical basis and practical guidance for improved performance of the normal sulfur sorbent and the choice of new sulfur sorbent.

然而传统的燃煤固硫剂石灰石,因钙转化率低而使该技术的推广应用受到限制,在实验研究的基础上,对钙基材料—石灰石和贝壳用于燃煤的煅烧固硫反应过程进行数学建模与分析,以便为改善常规固硫剂的性能,选择新型固硫剂提供理论依据和实用指导。

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