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Objective:To observe the effect of Jiawei Zhenwu Tang on rat model of hepatorenal syndrome.

目的:研究加味真武汤对硫代乙酰胺所致肝肾综合征模型大鼠肝肾功能的影响。

Methods: The rats were injected with thioacetamide 600mg/kg twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the normal saline hypodermically.

按600mg/kg的剂量经皮下注射硫代乙酰胺1次,24小时后按相同剂量重复注射1次,造成大鼠急性肝衰竭模型,于实验前3天至实验结束分别给予各组大鼠生理盐水,不同剂量丹黄方及促肝细胞生长素,观察造模后48小时内大鼠病死率,并于造模48小时后经腹主动脉采血测定ALT、AST、TBil、TNF-α、IL-6,取肝组织作病检。

The content of endogenous hormones, including IAA, ABA, GA3 and ZT, and phenol compounds were measured using HPLC, anti that of total sugar and total nitrogen were analyzed by the methods of hyposulphite titration and nitrogen.

采用高效液相色谱法测定插穗内源激素(IAA、ABA、GA3和ZT)和酚类化合物的含量,硫代硫酸钠滴定法测定总糖和凯式定氮法测定总氮含量。

Only Cr reacts with DDTC to form a 1: 3 complex which is then extracted with methyl isobutyl ketone and determined by flame atomic absorption spectroscopy.

本文拟定的方法是在pH-4条件下,用二乙胺硫代甲酸钠与甲基异丁基甲酮络合萃取铬,用原子吸收光谱法测定。

Objective To evaluate and compare the ability to detect tumor by bis (N-ethoxy-N-ethyl dithiocarbamato) nitrido 99mTc [ 99mTcN 2 ] and 99mTc hexakis-2- methoxyisobutyl isonitrile [ 99mTc-MIBI ].

目的 探讨99mTc-氮-二(N-乙基-N-乙氧基二硫代氨基甲酸盐)[99mTcN2]应用于肿瘤诊断的可能性并与99mTc-甲氧基异丁基异腈(99mTc-MIBI)肿瘤模型显像进行比较。

Furthermore, recent res earches have found out that isothiocyanates have the anticancer effects by usi ng the inhibiting and repressing factors.

近年来的研究发现,异硫代氰酸盐以阻遏和抑制因子发挥抗癌作用。

The myrosinase gene was amplified from total RNA by RT-PCR and inserted into Escherichia coli expression vector pGEX-4T-1.The result of sequencing was EF583560 in GenBank.

实验通过RT-PCR程序从提取的油菜总RNA中扩增出硫代葡萄糖苷水解酶(又称黑芥子酶,EC3.2.1.147)基因,酶解后插入大肠杆菌表达载体pGEX-4T-1,获得克隆菌株。

Influences of two temperatures (4 and 20℃) on weight loss, carotenoids andGS contents in pakchoi shoots were investigated during storage.

5研究了不同贮藏温度(20℃,常温;4℃,低温)对小白菜失重率、叶绿素、类胡萝卜素和硫代葡萄糖苷含量的影响。

Methods: rMSCs were separated from rib marrow with Ficoll Paque reagent and expanded in culture medium. Differentiation of rMSCs into neurons were induced with DMEM/monothioglycerol.

采用Ficoll Paque液(1.083 g/L)离心分离兔MSC,体外扩增,硫代甘油等试剂的无血清达乐伯克改良必需基本培养基诱导MSC分化为神经元。

Methods K-ras gene point mutation and its style at codon 12 of Patu 8988 cell were detected by using PCR-SSP and sequence analysis.

方法顺序特异引物聚合酶链反应法和基因测序检测Patu8988细胞K-ras点突变形式,根据点突变形式设计并合成硫代反义寡核苷酸(K-ras mutation ASODN)作用Patu 8988,通过噻唑蓝比色法检测细胞生长情况;流式细胞术检测K-ras蛋白表达和细胞凋亡;逆转录-聚合酶链反应检测K-ras mRNA表达水平;以Patu8988细胞建立裸鼠胰腺癌模型观察K-ras mutation ASODN在体内的抗肿瘤效果。

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