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The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2/6/7病毒样颗粒,待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解,培养基上清和细胞裂解液样品两次40%蔗糖垫93000g超速离心,沉淀样品经SDS-PAGE胶分离,用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性,电镜检测重组病毒样颗粒的形态结构。

Chemical products containing gold and silver, including gold chloride, gold and potassium cyanide, gold water, silver nitrate, silver oxide, silver chloride, silver iodide, silver bromide, etc.

含金银化工产品:包括氯化金、氰化金钾、金水、硝酸银、氧化银、氯化银、碘化银、溴化银等。

A method for the determination of water soluble chloride content in iron ore by potentiometric titration was studied.

以2g/L硫酸钾为溶解试剂,硝酸银为滴定剂,电位滴定法测定铁矿石中的水溶氯,通过电极电位-浓度二次微商滴定曲线,计算二次微商等于零时对应的V为滴定终点,可省去绘制E-V曲线或ΔE/ΔV-V一次微商曲线作图带来的麻烦。

Chlorine contents of the representative composition of MSW are quantitatively analyzed by using silver nitride volumetric analysis method, mercury thiocyanate spectrophotometer method and ion chromatography method etc, the results have been tabled and the determination methods are confirmed.

文章首先分析了入炉垃圾中PVC类的主要赋存形态,常见塑料的用途和种类,选用有代表性的垃圾组分采用离子色谱法、硝酸银容量法和硫氰酸汞分光光度法测定垃圾组分含氯量,得到了常见垃圾组分的含氯量数据,确定了不同垃圾组分的含氯测定方法。

The silver nanoparticles were characterized by using X-ray diffraction,energy dispersive spectrometer and scanning electron microscope.

在70℃时采用聚乙烯吡咯烷酮和氢氧化钠的混合水溶液,利用葡萄糖还原硝酸银制备了银纳米颗粒。

Siler sulfadiazine is used to treat serious burns. Fabric impregnated with siler is sometimes used as a dressing for wounds or skin infections. And siler nitrate is occasionally used to treat warts and corns.

磺胺嘧啶银用于治疗严重烧伤;用银浸渍的纺织品有时可用于包扎伤口或皮肤感染;而硝酸银可偶尔用于治疗疣和鸡眼。

Past 30,Jewelcrafting items seem to be at roughly a"quest reward"level,and as of yet there are no"ringers"that would warrant taking off dungeon loot for.

超过30级后***加工的装饰品被副本硝酸银溶液战利品远远的超过,还没有发现那些可以超越副本战利品的东西。

Results Well-resolved,reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established.Silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.Gels stained with colloidal Coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.

结果 获得了背景清晰、重复性好的双向凝胶电泳图谱,两种染色凝胶相比,硝酸银染色在样品少时显示更佳,过量则影响图像质量,而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加,并无明显拖尾。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr,用双向凝胶电泳技术分离总蛋白,银染及胶体考马斯亮蓝染色,image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱,两种染色凝胶相比,硝酸银染色在样品少时显示更佳,过量则影响图像质量,而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加,并无明显拖尾。结论两种显色方法受样品量影响较大,恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

Dao first art paint line刮干净Department, the lead Department in his wax-coated, and then along the alignment pin is zoned to the wax, then drop in on top of silver nitrate solution.

先用美工刀把断线处的漆刮干净,在露出的导线处涂上蜡,再用针顺着走线把蜡划去,接下来就是在上面滴上硝酸银溶液。

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