硝化菌

After years' effort, a new aerobic denitrifier that can deoxidize NO 3 - to N 2 and N 2 O has been isolated from ocean environment. Compound bacterial production is made up of aerobic denitrifying bacteria, nitrifying bacteria and organic compound degrading bacteria.

本项目将好氧还硝化菌、硝化细菌和有机质高效降解菌制成复合微生物制剂，通过多种微生物共同作用，在好氧的条件下能同时进行有机质降解、硝化作用和反硝化作用，可有效降低水中的三氮（ NH 4 +、 NO 2 -、 NO 3 -）。

According to the above study results, especially the steady operation of two-sludge system（2A/O+N 2SBR）, it can be proved that denitrification and excess phosphorus uptake are relatively independent and intersectant biological processes. The overlap is that denitrifying phosphorus removing bacteriawhich possesses the two bacterial metabolisms, could utilize nitrate as an electron acceptor for phosphorus uptake. The two-sludge system can not only combined biological phosphorus removal and denitrification processes to form a process in practical wastwater treatment , but also solved the main difficulties of current single-sludge systems:①the sludge age competition between phosphorus removing bacteria and nitrifier;② the substrate competition between denitrification and dephosphatation.

以上的研究结果，尤其是时间序列的双泥生物反硝化除磷脱氮系统工艺（2A/O+N 2SBR法）的稳定运行，不仅证明了生物脱氮与生物除磷是两个既相对独立又相互交叉的生理过程，其交叉点是同时拥有硝酸盐还原性和超量吸磷这两种生化特性的细菌进行的反硝化吸磷脱氮生化反应，而且双泥系统工艺克服了常规单泥生物脱氮除磷工艺的两大问题（①聚磷菌和硝化菌的SRT相互干扰；②反硝化与生物除磷竞争VFA），同时保证了脱氮和除磷效果，排水指标达到污水综合排水标准（GB8978-1996）的一级标准，具有实际工程使用价值。

Nitrite oxidoreductase was responsible for the nitrification of nitrite-oxidizing bacteria, which was coded by norB gene and others.

硝化菌主要由两大生理群组成：氧化氨氮为亚硝酸盐氮的是亚硝酸菌；氧化亚硝酸盐氮为硝酸盐氮的是硝酸菌，硝酸菌中负责硝化作用的酶是亚硝酸氧化还原酶，这个酶由norB等基因编码，因而对这个酶和基因的研究将有助于氮污染的控制和防治。

Raise bacterium from different, inferior nitrify bacterium and nitrify bacterium 3 person in light of the value of SOUR, different raises bacterium active to be compared in the lower level of filter strong, and inferior nitrify bacterium, nitrify bacterium raise bacterium to be in oneself medium, upper levels of the system is more active.

从异养菌、亚硝化菌和硝化细菌三者的sOUR的值来看，异养菌活性在滤池的下层比较强，而亚硝化菌、硝化菌等自养菌则在系统的中、上层比较活跃。

According to the above study result, the diversity of microorganisms population in the SUFR system is complex and the bio-community formed in the SUFR system is stable. The close relations between abundance of bacteria and nutrients were found. The correlation between heterotrophic bacteria and COD was 0.949. The correlation between organic phosphate bacteria and TP was 0.815. The correlation between nitrosobacteria and NH3-N was 0.909. The correlation between disnitrifier bacteria and TN was 0.653. These inneglectable great factors effecting on phosphorus-uptake and phosphorus-release of phosphorus accumulating organisms are the influent COD、the concentration of DO、the sludge retention time、the temperature. The diversity of organic phosphate bacteria population in the SUFR reactor was complex and there is no phosphate bacteria taking the absolute superiority in quantity. The near-native pure culture method powerfully complement the traditional pure culture technique and enrich culture technique. The sludgy bioactivity is very good in whole SUFR reactor. By the quality of being biochemistry, the anaerobic phase is higher than anoxic phase and the anoxic phase is higher than aerobic phase of SUFR.

通过以上试验内容研究发现，SUFR 系统中的微型动物种群较多，微生物生态系统稳定；系统中微生物的数量与营养盐的含量密切相关，其中总异养菌与COD的相关系数r 为0.949，有机磷细菌与TP 的相关系数r 为0.815，亚硝化菌与NH4+-N的相关系数r 为0.909，反硝化菌的数量和TN 的相关系数r 为0.653；污水生物除磷工艺中进水COD 的浓度、DO 浓度、泥龄的长短、温度都是影响聚磷菌释磷及吸磷效果的不可忽视的因素；SUFR 系统中的磷细菌呈现种群多样化的趋势，没有占绝对优势数量的磷细菌；微孔滤膜近自然培养法是对传统纯培养技术和富集培养技术的有力补充；整个SUFR 反应器系统中污泥的生物活性很好，就可生化性来讲，厌氧反应器大于缺氧反应器大于好氧反应器；按照动力学方程式求出的细胞生长动力学特征值和SUFR 脱氮除磷系统工艺的实测值结果基本吻合。

Experimental results showed that, in this process, the optimal pH of the shortcut nitrification was 8.0～8.5; when the pH was less than 6,the nitrification is completely inhibited; when the pH was 6～7, at the end of aeration, the Nitrobacteria become the dominant bacteria because of the dropping of the free ammonia concentration. It is known to all, the activity of the Nitrobacteria and Nitrosomonas was affected by FA concentration, so nitrification was affected by FA concentration. According to the author's research results, the FA concentration which inhibit the Nitrobacteria was below 1.0mg/L,which inhibit the Nitrosomonas was above 10mg/L.

实验研究结果表明：本工艺中，短程硝化反应的最佳pH值在8.0～8.5之间，当pH小于6时，整个硝化反应都会受到抑制，当pH在6～7之间时，随着游离氨浓度的降低，在曝气后期，硝化菌将会占据优势；FA浓度对短程硝化反应的影响是通过对亚硝酸菌和硝酸菌的抑制来实现的，在本研究条件下，游离氨对亚硝酸菌的抑制浓度在10mg/L以上，对硝酸菌的抑制浓度在1.0mg/L以下；温度对短程硝化反应的影响是双重的，既影响微生物的生理活性，又影响FA浓度。

Five different selective culture medium were used for sewage microorganisms directional isolated cultivate, advantage compound bacteria of photosynthetic bacteria, ammonifying bacteria, nitrifying bacteria, denitrifying bacteria and phosphate solubilizing bacteria could be gained through enrichment cultivate, then compounded them and used in test wastewater static treatment.

采用5种选择性培养基对水样中的微生物进行定向分离，通过富集培养，可以得到光合细菌、氨化菌、硝化菌、反硝化菌和磷细菌的优势菌群，进行复合后，再对试验污水进行静态处理。

The population distribution of physiological groups of bacteria,including ammonifying bacteria, denitrifying bacteria, nitrobacteria and nitroso bacteria, organic ph

用最大可能数法和平板计数法，于2002年1月～2003年3月对苏州河水体和底泥中的主要微生物功能菌群——包括有机磷分解菌、无机磷分解菌、氨化菌、亚硝化菌、硝化菌和反硝化菌等进行了生态调查，并分析探讨了它们在苏州河水生态系统中的作用。

The population distribution of physiological groups of bacteria,including ammonifying bacteria, denitrifying bacteria, nitrobacteria and nitroso bacteria, organic phosphate dissolving bacteria and inorganic phosphate dissolving bacteria in water body and sediment of Suzhou Creek are studied with MPN and flat account method from Jan. 2002 to Mar. 2003. The role of these physiological groups of bacteria in Suzhou Creek aquatic ecosystem is discussed.

用最大可能数法和平板计数法，于2002年1月～2003年3月对苏州河水体和底泥中的主要微生物功能菌群——包括有机磷分解菌、无机磷分解菌、氨化菌、亚硝化菌、硝化菌和反硝化菌等进行了生态调查，并分析探讨了它们在苏州河水生态系统中的作用。

This product is composed of high-effect compound bacillus, photosynthetic bacteria, nitrifying bacteria, denitrifying bacteria, sulfurizing bacteria etc.

本品由高效复合芽孢杆菌、光合菌、硝化菌、反硝化菌、硫化菌等有益菌群组成。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。