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硝化细菌

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This paper is to find out the best condition of the immobilized microbial beads to treat NH+4-N in fishery wastewater ,which is made from entrapping nitrobacteria with chitosan and alginate as entrapment carriers.

用壳聚糖和海藻酸钠作为载体,包埋固定化硝化细菌,制备固定化小球,处理养殖水体中的氨氮,寻找了固定化硝化细菌小球的最佳工艺条件。

Nitrobacteria in the sediment in Liaohe estuarine were investigated by MPN-Griess and MPN-PCR in spring, summer and autumn of 2007. The summarization of the results on temporal and spatial distribution of nitrobacteria in sediment was done.

采用MPN-Gtiess和MPN-PCR两种方法,于2007年春季(5月份)、夏季(8月份)和秋季(11月份)分3个航次,对辽河口区域沉积物中的硝化细菌进行了计数,并对硝化细菌在时间和空间上的分布进行了定量分析。

The effects of immobilized microbes including Rhodospirillaceae, Bacillus, Nitrobacteria and denitrifying bacteria on the changes in species, quantity and biomass of algae and zooplankton in aquiculture water and on the physicochemical environmental factors, such as nitrogen, were studied.

研究了固定化微生物(含有光合细菌、芽胞杆菌、硝化细菌以及反硝化细菌)对养殖水体中藻类和浮游动物的种类、数量、生物量以及水体氮素等理化环境的影响。

The mount of germ and fungus quantity in soil are remarkably increased for the continuous cropping about 5 years, puts the trichobacteria quantity always change tendency to reduce;The edaphon total quantity, the bacterium quantity change reduces generally above the continuous cropping 5 years and below 5 years (including 5 years) the change are not obvious;In the nitrogen physiology group by ammoniates the bacterium primarily and the quantity changes are not very big;The nitrifying bacteria quantity is lower than the comparison (non- continuous cropping), the quantity of denitrifying bacteria is opposite;The alkalinity phosphatase, the urease, invertin activeness have a closed relations with the continuous cropping when it will increase less than 5 years or decrease in more than 5 years. The relation between catalase activeness and the continuous cropping is not very big. The reduction of the invertin activeness, the alkalinity phosphatase, urease activeness have slightly elevates, catalase activeness have no the regular changes.

表现为连作条件下土壤真菌数量显著增大,放线菌数量总的变化趋势降低;土壤微生物总量、细菌数量变化在连作5年以上普遍降低,5年以下(包括5年)则变化差异不明显;氮素生理群中以氨化细菌为主,但数量变化不大;硝化细菌数量低于对照组,反硝化细菌数量则相反:多酚氧化酶、转化酶活性在5年之前逐渐上升,5年之后逐渐下降,,脲酶活性随着连作年限增加持续上升,过氧化氢酶活性、碱性磷酸酶活性变化无规律。

The number of autoterophic bacteria were significantly lower than the heterotrophic bacteria. Majority of 21 strains of heterotrophic bacteria that isolated from water body were Pseudomonas. Majority of 25 strains of heterotrophic bacteria isolated from sediment were bacillus, microccus and Pseudomonas.Three genus of autotrophic bacteria were sulphuret bacteria, nitrify bacteria and nitrosobacteria that were capable of oxidizing H2S, S, FeS, oxidizing NH3 to NO2 and NO2 to NO3 respectively.

试验稚鳖池水中共分离出21株异养细菌,其中优势菌为假单胞杆菌属细菌;底泥中共分离出25株异养细菌,其中优势菌为芽胞杆菌属细菌,微球菌属细菌假单胞菌属细菌,三种自养细菌分别为具有氧化硫化氢、元素硫或硫化亚铁的硫细菌;具有氧化氨为亚硝酸的亚硝化细菌和能将亚硝酸进一步转化成硝酸的硝化细菌

The number of heterotrophic bacteria and nitrifying bacteria increased with the time of the hydraulic retention time, contrary to the nitrobacter.

根际细菌和硝化细菌数量随着水力停留时间增加而增加,而根际反硝化细菌数量则减少。

In addition, under the above-selected nitrosification condition, the immobilized cells N4 and B08 were used for the combined short-cut biodenitriflcation with the ratio of immobilized cells 1:1 and the seed volume 15%, 27h the denitrogenation efficiency reached 97.53%; the immobilized cells N4 and B08 were used to do denitrogenation application test on eutrophic lake water of our school, 12h the NH4+-N removal rate was 84.06%.

在以上所选亚硝化条件下,按1:1的比例,亚硝化细菌N_4和反硝化细菌B_(08)固定化细胞接种量为15%,广西大学硕士学位论文固定化微生物捷径生物脱氮的研究作联合捷径脱氮试验,27h脱氮率为97.53%;并作本校富营养化湖水的脱氮应用试验,处理12h,NH4十一N去除率为84.06%。

In this paper, adopted augmenting culture in nitrite bacteria culture media and the method of silica gel plate isolation,31 strains of bacteria were isolated from vegetable garden soil of our school. The color reaction test was carried out with Griess reagent and culture fluid, which was regarded as the index to determine producing NO2- or not. 13 strains which color were deep were obtained and they were further rescreened by doing nitrite test. A strain N4(coded N4,the same to the following)with higher rate of nitrosification was picked up after rescreened; A strain B08( coded B08 ,the same to the following) with higher rate of denitrification was obtained after isolated and rejuvenated from our lab conserving mixed denitrifying bacteria culture.

本研究采用亚硝化细菌富集培养基选择培养和硅胶平板分离法,从本校农场菜园土中分离到31株细菌,以格利斯试剂对培养液的反应颜色深浅作为指标衡量其产NO_2~-的多少,经初筛从分离株中筛选出13株格利斯试剂反应颜色较深的菌株,再对这13株菌作亚硝化试验,最终选出一株亚硝化速率较高的菌株(编号为N_4,下同);另外,通过对本实验室保存的反硝化细菌混合菌液进行分离复壮,筛选出一株反硝化速率高的菌株编号为B_

However, nitrite nitrogen was observed to accumulate in the MBR effluent during the maturation of nitrosomonas group and the start-up accomplishment lagged 6 days behind.

而在亚硝化细菌的成熟过程中MBR出水中出现了NO2-N的积累,硝化细菌的成熟滞后了6d。

The distributions of microbes in various physiological groups had bigger difference in the 5 soil types, n which, the proportions of ammonifying, nitrite, and denitrifying, aerobic dinitrogen fixed bacteria were relatively bigger and that of sulfate-reduction and inverse-sulfuration bacteria were relatively less.

土壤各生理类群微生物在5种类型土壤中的分布有较大差异,氨化细菌、亚硝化细菌、反硝化细菌和好氧固氮菌在5种类型土壤中占有的比例相对较大,硫化细菌和反硫化细菌相对较少。

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