破骨细胞

Methods Murine RAW264.7 and SP 2/0 cells were co-cultured in DMEM containing dexamethsone and 1, 25 2VitD3, and the mature of osteoclasis was identified by TRAP staining.

将小鼠RAW264.7和SP2/0细胞在含地塞米松和1，252VitD3的破骨细胞诱导分化液中混合培养，通过TRAP染色鉴定破骨细胞的成熟。

Objective To develop a method for quantitative determination of biological activity of osteoclast suppressor using osteoclast as a model.

目的 以破骨细胞为模型，建立抑制破骨细胞功能药物的生物活性定量测定方法。

Objective To establish a method for human osteoclast marrow culture on the base ofpreviously published method.

目的 在国内前人建立动物破骨细胞骨髓培养方法的基础上，首次建立人破骨细胞骨髓培养法。

Bone is a complex tissue composed of several cell types and the two major of them responsible for bone remodelling are osteoclast, which resorb bone, and osteoblast, which form new bone.

中文摘要骨骼是具有数种细胞型态的复杂组织，而其中最重要的两种分别是负责骨吸收作用的破骨细胞及职司骨生成的造骨细胞。

Semiquantitative reverse transcription PCR method was used to determine the expression level of MMP-9 mRNA and MMP-8 mRNA.

结论不同浓度的雌激素呈时间和剂量依赖性地抑制破骨细胞的活性，对兔破骨细胞基质金属蛋白酶表达的调控作用随其浓度变化而不同。

We review the effect of rare earth ions on bone metabolism as the following three aspects:(1)effect of rare earth ions on differentiation and function expression of osteoblasts and osteoclasts;(2)effect of rare earth ions on the osteogenic and adipogenic differentiation of bone marrow stromal cells;(3)effect of rare earth ions on bone mineral phase of animals.

本文回顾了稀土离子对骨代谢影响的研究进展，探讨了其药用和安全性问题，主要从以下三个方面进行综述：(1)稀土离子对成骨细胞细胞和破骨细胞的分化和功能表达的影响；(2)稀土离子对骨髓基质细胞成骨分化和成脂分化的影响；(3)稀土离子对动物骨矿物相的影响。

Immunohistochemical staining revealed that expression of RANKL and OPG protein was detected in osteoblasts, bone lining cells, fibroblasts and osteoclasts which mostly located in resorption lacunae.

免疫组化结果显示，RANKL和OPG的阳性表达位于牙周成骨细胞、骨衬里细胞、牙周纤维细胞和位于骨陷窝内的破骨细胞中。

After cultured for 4 days,the cells were stained with TRAP. Multinucleated(≥ 3 nuclei) TRAP-positive cells were counted as osteoclasts, and the expression of TRAP mRNA were determined by RT-PCR.

2.0？10.0？50.0 ？滋g/ml，对获得的各组破骨细胞行抗酒石酸酸性磷酸酶染色计数阳性破骨细胞(核≥ 3)的数目；提取总RNA用逆转录-聚合酶链反应检测TRAP mRNA的表达？

In general, osteoporosis is a disease of bone which is caused by an imbalance between osteoblastic bone formation and osteoclastic bone resorption. However, the recent research showed that the ability of bone marrow mesenchymal stem cells differentiates into adipocytes is greater than osteoblasts and it was thought to cause into osteoporosis, such as diabetes.

骨质疏松症一般被认为是细胞的骨生成作用与破骨细胞的骨吸收作用失去平衡，然而近年来的研究指出骨头内的间质干细胞分化成造骨细胞的能力减弱或是间质干细胞分化成脂肪细胞的能力增强，这样的结果往往会导致骨质疏松症，如糖尿病状态。

In general, osteoporosis is a disease of bone which is caused by an imbalance between osteoblastic bone formation and osteoclastic bone resorption. However, the recent research showed that the ability of bone marrow mesenchymal stem cells differentiates into adipocytes is greater than osteoblasts and it was thought to cause into osteoporosis, such as diabetes.

中文摘要骨质疏松症一般被认为是细胞的骨生成作用与破骨细胞的骨吸收作用失去平衡，然而近年来的研究指出骨头内的间质干细胞分化成造骨细胞的能力减弱或是间质干细胞分化成脂肪细胞的能力增强，这样的结果往往会导致骨质疏松症，如糖尿病状态。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。