破骨细胞

Collagenase and 25 m filtration web, and characterized in terms of the expression of different phenotypic markers of osteoclasts and their ability to resorb bone matrix. The phenotypic markers of DCs include the expression of tartrate-resistant acid phosphatase, acid phosphatase, resorption pit assay, reaction to calcitonin and identification of F-actin.

于37oC，5％CO。培养箱中培养1－2小时后，弃培养液：lxPBS清洗数次；0.25％胰酶－EOTA 37℃消化5分钟，加入含川％FBS的a－MEM，经小25tim过滤网过滤；IX PBS清洗末过滤细胞，即得到纯化的破骨样细胞，并进行细胞形态学和分于生物学鉴定。

However, the mechanism how PDL cells transform mechanical signals into the activity of bone remodelling has been unclear.

但是正畸过程中牙周膜细胞受机械力后如何将力信号转化为成骨及破骨活动的机理至今仍不清楚。

Bone resorbing osteoclasts contain high amounts of tartrate-resistant acid phosphatase 5b and secrete it into the blood circulation.

破骨细胞重吸收骨骼时包含大量的抗酒石酸酸性磷酸酶（TRACP 5b）并且分泌到血液循环之中。

In women at the menopause, blood levels of the female hormone oestrogen start to decrease.

女性在更年期内，血液中雌激素的水平开始降低，增加了破骨细胞对骨骼的分解作用，导致骨骼密度的降低。

E2 can down-regulate the expression of CK mRNA in OCLs, but has no effect on the expression of RANK or CTR mRNA.

217β-E_2可下调破骨样细胞CK的表达，而对RANK和CTR表达量无明显作用。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。