破坏地

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The nanoplex were smaller than the unmodified nHAP; thelipiodol/nanoplex emulsion, with the mean diameter at 75±11.8nm can disperse moreuniformly and be more soluble and stable, contrary to the unmodified nHAP and nanoplex Conclusion: Nanoplex/pDNA mass ratios greater than 15 had exhibited completepDNA absorption onto positive charged polyplex and pDNA protection from thedestruction of nucleinase in rabbit serum. Unmodified nHAP may can\'t induce effectivegene delivery.PartⅡThe construction of the recombint eukaryotic expressive vector ofwild-type human p53 and RbObjective: To construct the recombint eukaryotic expressive vector of wild-type humanp53 and Rb by utilizing the theory of molecular medicine.

结果nHAP颗粒本身大小小于100nm，但由于颗粒表面本身带负电，不能包括有效地结合及保护DNA；经Ca~(2+)修饰后，颗粒可带正电，可一定程度地结合和保护DNA，但聚合物本身的中性电与带负电的细胞膜结合，而且本身的颗粒则变地容易聚集而成为很大的颗粒，也不利于细胞的吞噬；经PLL修饰后，颗粒溶解性提高，直径变小，表面带正电，电泳结果显示PLL-nHAP颗粒可包括有效地结合和保护DNA免受兔血清的破坏，而且复合物表面的正电也包括有利于与细胞膜的结合与吞噬。

In the generalization of contact lens develops day by day, on the issue associated with corneal infection due to incorrect habit of contact lens is put much emphasis. Pseudomonas aeruginosa is one of the most important infectious pathogens, it will adhere on the cornea and destroy the corneal tissue opportunistically with its quite violent virulence. The infected corneas will be attacked to necrosis by one to two days with very fast destructive speed. It is very important to diagnosis the infectious pathogen in time.

在隐形眼镜愈来愈普及的趋势下，因隐形眼镜配戴习惯不正确造成角膜感染的议题也逐渐受到重视，而绿脓杆菌正是其中最重要的一种感染病菌，其会附著在角膜上，然后伺机地破坏角膜组织，绿脓杆菌毒性相当猛烈，故其破坏的效率相当地快，角膜大概一至二天就会被摧残溃烂，因此即时诊断出角膜感染的病原体是相当重要的。

You have destroyed your vow to Eul when you met on that fateful summers noon as a young and lost map developer who didn't have a true purpose in the world and decided to ask those with more wisdom because you were afraid of being unable to live up to the great expectations bestowed upon you, down at the serene windy lake with it's glassy surface and lone fisherman in a small and easily overturnable boat hovering quite near the center worrying about feeding his family and the deers trotting off in the distance being jealously eyed by small rabbits who envied their youthfulness but at the same time were glad they were such innocent symbols of cuteness that were sometimes ignored by the American MEDIA, you have destroyed the oath you took with him as he decided to leave the glowing jeweled crown with the more exuberant youth of his dying generation.

在那个命运中的夏午，当时还是一个年轻且彷徨的地图开发员的你破坏了你和Eul的约定。因为你在世上没有一个确定的目标。所以你决定去请教那些更有智慧的家伙们，因为你无法承受如此大的期待而你为此感到恐惧，低着头看着碧波荡漾的湖水、那玻璃一样的湖面的非常靠近中心的位置有一个乘着简陋的随时都有沉船危险的轻舟的孤独的渔夫正在为养活他的家庭而感到担心。林中的小鹿们踏着碎步而在另一边小兔子们则充满嫉妒地注视着它们。兔子们羡慕它们的活力同时又为自己的可爱能作为纯真的象征而感到高兴。因为有时这一点能够迷惑美国媒体，你已经破坏了你和他在他决定把镶嵌着炽热宝石的皇冠留给更有朝气的年轻人作为他的继承人时的誓约。

Then the sustainable development of Qitai artificial oasis was discussed. Researches showed that the oasis development has gone through three phases: The first was harmonic stage before the middle of 20, the oasis developed according to water resources. The second was unharmonic stage from 1950's to 1980's because of unreasonable policies, that is cultivated area enlarged rapidly and water resources exploited backward. As a result, land output was lower, and environment destroyed heavily. The third stage is after 1980's, the new fragile balance between oasis and its environment was established on the base of the more and more ground water utilization.

研究认为奇台人工绿洲人地关系发展经历了三个阶段：第一阶段是20世纪50年代以前，&以水定地&的相对协调发展时期；第二阶段是20世纪50-80年代人地关系发展失衡时期，人工绿洲迅速扩张，水资源开发滞后，耕地因缺水灌溉而成为低产田，环境破坏严重，人地发展失衡的原因是政策因素；第三阶段是20世纪90年代以来人地关系脆弱平衡时期，决定其平衡的因素是不断增加的地下水开采量。

The results showed that the failure area extended, the failure probability increased greatly, and the reliability index decreased greatly when water was considered. It is concluded that water has unignored effect on slope stability.

研究表明，地下水的存在使边坡的破坏面积增大，破坏概率剧增，边坡可靠性指标极大地降低，因此地下水对节理边坡稳定性具有不可忽视的作用。

One who willfully or maliciously defaces or destroys public or private property.

破坏他人财产者故意或恶意地''。'毁坏'。''或破坏公共财产或私有财产的''。'人'。

They are dislodged by new industries, whose introduction becomes a life and death

古老的民族工业被破坏了，或者正在被每天地破坏。

Traditional architecture is the most important tangible cultural heritage, an important carrier of our culture and memory and valuable non-renewable wealth, so it should be properly protected, but unfortunately, the vast majority of China's architectural heritage has been destroyed, and now the destruction is still going on.

传统建筑是最重要的物质文化遗产，是我们文化和记忆的重要载体，是不可再生的宝贵财富，应该得到妥善地保护，但是非常可惜的是我国的绝大部分建筑遗产都被破坏了，而且现在这样的破坏还在继续。

The assembly of journal and plain bearing liner space is too little, force of organic still oil pressure is small it is the crucial index that affects lubricant result, make lubricate because of lacking engine oil between crankshaft and plain bearing liner and bite dead invalidation, include to be pulled badly, lack lube, layer of alloy of dry attrition generation is fused, grind sliding surface to did not maintain the oily film of certain ply, and all sorts of local overload exhaustion are destroyed, cavitation erosion and V form are destroyed etc, through technology of him director diesel locomotive old experience reachs the analysis of pair of its breakdown reasons, put forward precautionary measures especially. As a result of,the burns caustic mechanism plain bearing liner to burn caustic mechanism of 1 plain bearing liner is the lube film burst between journal and plain bearing liner, cause plain bearing liner and crankshaft generation clash, below high temperature, high pressure and high rotate speed, the wear-resisting alloy layer of plain bearing liner wears away prematurely or fuse, cause plain bearing liner and crankshaft to stick thereby bite, can make the steel back of plain bearing liner and Kong Zhong produces whirligig, inlet port is jammed, carry on the back by steel again with the friction of aperture causes high temperature to make plain bearing liner burned-out further, can cause bolt to break off, happen swing a malign accident that pounds cylinder body of bad internal-combustion engine.

轴颈和轴瓦的装配间隙太小，还有机油压力低是影响润滑效果的关键指标，使得曲轴和轴瓦之间因缺少机油润滑而咬死失效，包括严重拉伤，缺少润滑油，干摩擦产生合金层熔化，磨滑面没有保持一定厚度的油膜，以及各种局部过载疲惫破坏，气蚀和V形破坏等，通过本人主管内燃机车技术多年经验及对其故障原因的分析，特提出预防办法。1轴瓦的烧损机理轴瓦烧损的机理是由于轴颈和轴瓦之间的润滑油膜破裂，导致轴瓦和曲轴产生摩擦，在高温、高压和高转速下，轴瓦的耐磨合金层过早地磨损或熔化，从而引起轴瓦和曲轴粘咬，会使轴瓦的钢背和座孔中产生旋转运动，进油孔被堵塞，再由钢背和座孔的磨擦进一步引起高温使轴瓦烧坏，并会造成螺栓折断，发生甩出捣坏内燃机缸体的恶性事故。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。