破伤风

Objective To obtain highly purified tetanus toxin fragment C with good immunogenicity.

目的获得高纯度、高免疫原性的破伤风毒素C片段蛋白。

Methods Express tetanus toxin fragment C in E.

均获得高纯度的破伤风毒素。

Purification of tetanus toxin was carried out by chromatography procedures.

用疏水层析和离子交换层析纯化破伤风毒素。

Will control the synthesis of tetanus toxin gene implanted in mice nerve cells, by regulating the toxin activity, the development of free mice brain circuit switching technology.

将控制破伤风毒素合成的基因植入实验鼠神经细胞，通过调节毒素活动，开发出可自由开关实验鼠脑神经回路的技术。

For example,1889-1890 years, the people studies the diphtheria toxin and the tetanus infection toxins with the immunology technique, using it again later on to study the plant toxin, such as the castor toxin, the croton toxin and snake poison, spider poison within the animal toxins.

例如，1889～1890年，人们用免疫学技术研究白喉毒素和破伤风毒素，随后又用它来研究植物毒素，如蓖麻毒素、巴豆毒素和动物毒素中的蛇毒、蜘蛛毒。

Interference with presynaptic vesicle fusion by exposure to tetanus toxin reverted functional to silent transmission, implicating SNARE-mediated fusion as a determinant of the ratio of NMDA:AMPA receptor activation.

以破伤风毒素干扰前突触链胞分泌，影响了无讯号传导，并显示以SNARE造成的分泌，决定NMDA受体与AMPA受体的激发的比率。

Now, research replaces the option of index in the various disease appraisement of curative effect to discuss , if with blood plasma 羟 hydrogen can appraise when ketone density is replacing index A flat kind ease pain medicine Kong Shi dose relieve pain effect , is taking ATT( aim at the antibody effect of tetanus toxin) as replacing index to appraise the immunity inhibition researchs of? single clone antibody 5c 8 of the CD 154 in monkey body to get better effect.

目前，已有研究就多种疾病疗效评价中替代指标的选择进行了探讨，如以血浆羟氢可待酮浓度作为替代指标评价阿片类镇痛药控释剂的止痛效果、以 ATT(针对破伤风毒素的抗体效应)作为替代指标评价猴体内 CD154的单克隆抗体5c 8的免疫抑制作用等研究都取得了较好的效果。

Methods Th multi-epitope peptide was made up of 5 Th epitopes(heat shock protein 65 of Mycobacterium tuberculosis p1-20,E2 protein of Rubella virus p54-65,engineering epitope PADRE,heat shock protein 60 of Chlamydia trachomatis p35-48,and tetanus toxin p830-843),and its gene sequence including of 219bp.

方法复合T辅助细胞表位由5个T辅助细胞表位组成(结核杆菌热休克蛋白65p120、风疹蛋白E2-4p5465、人工合成T辅助细胞表位PADRE、沙眼衣原体热休克蛋白60p3548、破伤风类毒素p830843)，其基因序列由219个核苷酸组成。

A multi-epitope antigen gene pcx was assembled by five highly conserved B-and/or T-cell epitopes, which were selected from C (1-16aa, 132-141aa), E1 (126-135aa), NS3 (139-147aa) and NS5B (768-775aa) protein, as well as a T-cell epitope of tetanus toxin, which was added to enhance T-cell immunity.

针对HCV的高度变异性及免疫特点，在HCV基因产物中优选了5个具有良好免疫原性的高度保守B／T细胞表位，组合成一个多表位抗原基因pcx，这些表位分别来自核心蛋白(1～16aa，132～141aa)，E1(126～135aa)，NS3(139～147aa)及NS5B(768～775aa)，以及破伤风毒素的T细胞表位用以增强T细胞免疫。

No month plague, SARS, polio, people infected with highly pathogenic avian influenza, dengue fever, anthrax, diphtheria, neonatal tetanus, leprosy, typhus, kala-azar, echinococcosis and wire Disease cases reported.

当月无鼠疫、传染性非典型肺炎、脊髓灰质炎、人感染高致病性禽流感、登革热、炭疽、白喉、新生儿破伤风、麻风病、斑疹伤寒、黑热病、包虫病和丝虫病病例报告。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。