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Methods Applying 1,25-_2D_3 induced osteoclast-like multinucleated cells and isolated mature rabbit osteoclasts cocultured with bone slices, the effects of icariin on the differentiation and bone resorbing activity of osteoclasts were investigated.

方法采用1,25-2D3诱导兔骨髓单核细胞形成骨细胞样细胞以及原代分离的乳兔成熟骨细胞与骨片共培养的方法,考察淫羊藿苷对骨细胞的分化及骨吸收功能的影响。

This study is to further investigate the mechanisms of osteoclastic bone resorption induced by IL-1 with established osteoclasts culture and osteoclasts, bone marrow stromal cells co-culture system.

本文利用建立的骨细胞培养和骨细胞、骨髓基质细胞共培养体系,在体外进一步研究IL-1诱导骨细胞性骨吸收的机制,旨在探讨细胞因子在骨质疏松症骨丢失发生中的作用。

The effect of PTH on the osteoclastic bone resorption,and how the osteo-blasts mediat the reaction was studied in vitro by using the method of isolating and cul-turing rabbit osteoclasts and osteoblasts.

采用分离、培养兔骨细胞和成骨细胞的方法,体外研究甲状旁腺激素对骨细胞骨吸收功能的影响,以及成骨细胞和骨细胞之间的相互作用。

The biochemistry and image analyzing were used to investigate the effects of the PDGF-AA at high concentration on the osteoclastic bone resorption in the osteoblast-osteoclast co-culture system.

在高浓度PDGF-AA的作用下,应用生化检查和图象分析技术,检测PDGF-AA对成骨细胞-骨细胞共育体系中骨细胞骨吸收功能的影响;应用免疫荧光检测骨细胞合成抗酒石酸酸性磷酸酶的变化;应用免疫组化和RT-PCR技术检测骨保护素配体的表达。

RESULTS: The time of misplacement of pedicle screw was 5-69 days with an average time of 18.5 days, including 7 cases of screw penetrating into lateral cortex, 4 of screw penetrating into medial cortex, 2 of screw penetrating into pedicle cortex, 2 of overplacement, 2 of intervertebral foramen placement and 2 of intervertebral space placement.

结果:患者自螺钉置入后至发现椎弓根螺钉误置时间为5~69 d,平均18.5 d。其中螺钉穿椎弓根外侧皮质7例,穿椎弓根内侧皮质4例,螺钉穿椎弓根皮质2例,螺钉置入过深2例,螺钉进入椎间孔2例,进入椎间隙2例。

Hand-folded four-Pack (50g/mdoes-quadratical and the following paper) or hand-fold three fold (60-80g/m-squared paper) in a manner worthy of the books which should be in the book-to-last three fold in a manner worthy of the fold sewup hemomediastinum, to rule out the air and in a manner worthy of the book to prevent wrinkles appear, but now there are many flats in manual folding, knowing very well that should be hemomediastinum, but the pursuit of production would go for the big is not hemomediastinum, and finally after the wrinkles in buchbinderei and unrectifiable is extremely serious.

手工折四折(50g/m平方及以下的纸张)或手工折三折(60-80g/m平方纸张)的书帖均应在书帖倒数第三折折缝上口,以排除书帖内空气而防止皱褶出现,但现在有许多单位在手工折页时,明知应口,但一味追求产量就是不口,最后书籍装订后皱褶极为严重而无法补救。

The sensitivity studies of break location show that the cold leg SBLOCA is more serious.

试验回路的口位置敏感性分析表明:在口尺寸相同的情况下,冷端口失水事故的事故后果更严重一些。

Third passage osteoblasts were plated at the density of 5×104/ml in 75ml culture flask, twenty four hours later treatment factors were added. Total RAN was extracted from osteoblasts using guanidine, and mRNA expression of OPG and RANKL were examined using RT-PCR.

OPG作为RANKL的诱骗受体阻断其与表达于骨细胞前体细胞和成熟的骨细胞表面的功能性受体RANK的作用,从而抑制骨细胞的分化和功能。

The third passage osteoblasts were plated at the density of 5×104/ml in 75ml culture flask, twenty four hours later treatment factors were added. Total RAN was extracted from osteoblasts using guanidine, and mRNA expression of OPG and RANKL were examined using RT-PCR.

OPG作为RANKL的诱骗受体阻断其与表达于骨细胞前体细胞和成熟的骨细胞表面的功能性受体RANK的作用,从而抑制骨细胞的分化和功能。

In this study, we could not find significantly less root resorption lacuna and significantly less TRAP activity in the IL-1 group than in normal group, but there was significant difference betweens TNFα group and normal group.

其中IL-1调控于骨细胞前体的分化阶段,TNF α调控成熟骨细胞功能激活阶段。2:RANKL/RANK调控系统既作用于骨细胞前体的分化、成熟,又作用于功能激活阶段。

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