研究动物

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

She sold chipmunks to Dawn Animal Agency for films, and provided numerous laboratories with animals for research.

她把花栗鼠卖给唐动物中介拍电影，为众多实验室的研究提供动物。

In this "five-step", the evolution of the first step in the series of groups of animals very strange: 14 more haikou insect specimens comparative anatomy studies show that, they not only, and the lack of chordomas in skin, muscle, breathing, circulation, neural organs such as the system with the fundamental difference between the existing chordates, One of the most distinctive is the gills of six foreign composed of respiratory system, and this is the mouth of higher animals in the gills.

在这分&五步走&的演化系列中，&第一步&的动物类群十分奇特：对1400多枚海口虫标本进行比较解剖学研究表明，它们不仅缺少脊索构造，而且在皮肤、肌肉、呼吸、循环、神经等器官系统上与脊索动物存在着根本差别；其中最为独特的是其由6对外鳃组成的呼吸系统，这与较为高等的后口动物的勤鳃迥然有别。

In this five-step, the evolution of the first step in the series of groups of animals very strange: 14 more haikou insect specimens comparative anatomy studies show that, they not only, and the lack of chordomas in skin, muscle, breathing, circulation, neural organs such as the system with the fundamental difference between the existing chordates, One of the most distinctive is the gills of six foreign composed of respiratory system, and this is the mouth of higher animals in the gills.

在这分&五步走&的演化系列中，&第一步&的动物类群十分奇特：对1400多枚海口虫标本进行比较解剖学研究表明，它们不仅缺少脊索构造，而且在皮肤、肌肉、呼吸、循环、神经等器官系统上与脊索动物存在着根本区别；其中最为独特的是其由6对外鳃组成的呼吸系统，这与较为高等的后口动物的内鳃迥然有别。

Dame Jane Goodall, DBE (born Valerie Jane Morris Goodall on 3 April 1934) is an English UN Messenger of Peace, primatologist, ethologist, and anthropologist.

珍。古德女爵士是英国灵长类动物学家、动物行为学家、人类学家和联合国和平使者、国际动物保育人士。珍。古德因45年长期致力於黑猩猩的野外研究而著名。

Not necessarily. Cat experts say felines are just different. They are solitary animals, motivated by the need to survive.

不尽然。猫的研究专家说猫科动物是不同的，他们是孤独的动物，由生存的需求推动。

A fence lizard or a 点击访问 http://club.topsage.com/forum-28-1.html 大家考研英语 desert iguana -- each cold-blooded -- usually has a body temperature only a degree or two below that of humans and so is not cold.

暖血动物包括哺乳动物和鸟类，其它动物统统被视为冷血动物。但是对更多物种进行的研究表明这种分类显然是不适当的。

Members of AAVP are teachers, students, scientists, veterinarians and others interested in parasites of companion, food-producing, and other domesticated animals and wildlife, some of which are transmissible to humans.

其成员主要是教师，学生，兽医学家和其它从事动物伙伴、食品加工和其它驯养和野生动物的寄生虫研究的人员，一些寄生虫可能会传给人类。

The result shown that,as feeding of fruit-eating bats and insert-eating bats,it is different in choosing the bat-bait,designing of bat-feeding box,feeding technique and method,feeding management.

野生动物饲养技术的发展，一方面为动物园展出动物的饲养提供技术保障，另一方面也为科学研究和科学实验提供技术支持。

Based on the model of Logistic, Levins and Tilman, the model of exponent, restoration of habitat,habitat overlap has been put forward systematically and probe into the following questions :how do the evolutional characteristic of meta-population in different regions, the relation between the extinction order of animals and the degree of habitat destruction upon by human activities.

本文主要研究了以下问题：1、不同区域集合动物种群的演化特点、动物物种灭绝的顺序与人类活动对栖息地破坏的&度&的关系2、人为增加或恢复部分栖息地，对不同区域集合种群里强弱不同的物种种群的演化、繁衍将产生什么影响？

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新