研究动物

Methods A model of autoimmune inner ear disease was established by systemically immunizing the guinea pig with the homologous crude inner ear antigen. The immunized cochleae and normal control cochleae were examined for the expression of hsp70 with techniques of immunohistochemistry and in situ hybridization.

利用同种异体内耳抗原免疫豚鼠，以建立自身免疫性内耳病（autoimmune inner ear disease， AIED动物模型，并应用免疫组织化学及原位杂位技术，研究热休克蛋白（heat shock protein， hsp70在正常对照组及 AIED 模型动物实验组耳蜗中的表达情况。

Squalene/Arlacel A(4:1)–MDP was used as adjuvant. First injection with 500 ug antigen was given to each animal, and three injection once two weeks with 250 ug antigen were followed. Serum Anti-ZP antibody response was evaluated by ELISA once two weeks. In eighth week after first injection, all female monkeys were encaged wi

在高等灵长类动物免疫研究证明去糖基卵透明带抗原免疫能产生抗生育作用，但不会引起卵巢功能紊乱等不良反应，糖基是导致免疫动物卵巢功能紊乱成分，为选择卵透明带抗原，用重组基因技术发展卵透明带避孕疫苗提供依据。

Based on the systematic research on investigation and taxonomy of Botany and Zoology as well as application of the indigenous knowledge to utilization and conservation of biodiversity, the families and species of mammals, distribution types and habitat distribution of birds, and faunas of amphibian and reptiles, types and characteristics of the fish in the area were investigated and analyzed.

基于系统的植物学和动物学调查和分类学研究，以及乡土知识在生物多样性的利用和保护方面的应用，对分布于保护区的哺乳动物的科和种、鸟类的分布类型和生境分布、两栖和爬行动物的区系、鱼类的类型和特点作了调查分析，讨论了分布于保护区的陆生和水生动物、动物的生态系统和多样性特征。

The research of foraging behavior ecology has a great significance in animal protection and artificial breeding.

非人灵长类动物觅食行为生态学的研究对动物保护、人工驯养和繁殖具有重要意义。

One such study, published recently in Animal Behaviour, shows not only that some do, but also that the presence of such traits is skewing the way data are collected by researchers.

最近发表在《动物行为》杂志上的一项研究表明，野生动物不仅具有个性特点，而且这些个性特点的存在使研究者们收集数据的方法也有了倾向性。

The maxillofacial and brain injuries were examined. Craniptomy was done to animals of impact injury 6 hours after damage, however it was done to animals of explosive injury in different time point (1 hour, 24 hours, 72 hours, 7 days, 14 days after damage). The pathological characteristics of corelative tissues were examined in detail under light and electron microscopes. The content of LPO, SOD, NO in soft tissue, brain, blood, and cerebral water content were measured, compared with control. The distribution and tissue location of NOS were studied by histochemistical and immunohistochemistic methods. Their relationship was studied to assess the pathological and physiological characteristics of maxillofacial injuries and brain damage associated.

撞击伤各组动物于伤后6h、爆炸伤各组动物于伤后不同时期（1h、24h、72h、7d、14d）开颅检查颅脑损伤情况，切取伤区肌组织、左侧脑组织、脑干标本行光镜、电镜观察；测定伤后肌组织、脑组织与血中LPO、SOD、NO含量及脑水含量变化；并使用组织化学和免疫组织化学的方法，观察伤后脑组织中NOS的分布和组织定位，研究伤后机体的病理及病理生理学改变特点，以及颅脑损伤与机体自由基反应、NO含量改变之间的关系，证实其病理作用。

Recent years, there were more and more researches on it. Daidzein, when used in livestock's diets, are claimed to improve animal growth, decrease feed cost, promote animal's reproduction and enhance body immunity. Owing to its wildly exist in legume crop and grass, the research and utilization of this compounds as new feed additives will have bright future.

大豆黄酮在畜禽日粮中的应用，能够较显著地促进动物生长、降低饲料成本、提高动物繁殖力和增强机体免疫力等，又因其广泛存在于豆类作物和牧草中，所以研究和开发这类化合物作为新型饲料添加剂将具有较大的潜力和广阔的发展前景。

The change of 〓 signal resulted in the disturbance of function in hypothalamuspituitary-adrenal axis and declination of immunity, and then the occurrence of symptoms in kidney Yang deficiency. Tonifying kidney herbs probably exerted an effect by regulating 〓 of hypothalamus and lymphocyte of blood.

本研究用醋酸可的松肌肉注射复制了肾阳虚动物模型，动物的表征类似于中医的肾阳虚症状，与文献报道的肾阳虚证表现相一致，说明本实验建立的肾阳虚大鼠模型是成功的，可用的。

So far, more than 60 invertebrate MTs have been reported each with two and more than two MT isoforms and variants respectively in most major invertebrate groups including nematodes, annelids, ech...

无脊椎动物物种间的金属硫蛋白存在着显著差异，研究无脊椎动物MTs多样性并揭示其生态服务功能，在理论与实践上都至关重要。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新