研究动物

We further evaluated the synergistic cytotoxicity of TRAIL in combination with actinomycin D, cisplatin and paclitaxel in A549 cell lines in vitro and in vivo.

本实验选择了人肺腺癌A549细胞瘤株作为我们实验研究的对象，通过TRAIL对人肺腺癌A549细胞诱导凋亡研究，在A549的荷瘤裸鼠的动物模型上研究了其体内的抗肿瘤活性，并进一步研究了TRAIL与化疗药物放线菌素D(actinomycin D，Act D)、紫杉醇和顺铂(cisplatin，CDDP)的联合作用对人肺腺癌A549细胞的体外协同抗肿瘤活性，通过化疗药物协同增强TRAIL抗肿瘤活性作用机制的初步研究，为降低临床化疗药的用药量，减轻其对机体的不良反应提供一定的理论基础。

The use of animals has been central to the development of anaesthetics, vaccines and treatments for diabetes, cancer, developmental disorders...most of the major medical advances have been based on a background of animal research and development.

使用动物对于麻醉学和疫苗的发展，对糖尿病、癌症和紊乱的治疗等极其重要。多数重要的医学都是以动物研究和开发的背景为基础的。

Carabid beetle was selected as bioindicators and pitfall trapping was used as sampling method for animal biodiversity evaluation; for flora evaluation, both field experiment and releve approach were used with assistance of remote sensing image; for the evaluation of soil organic carbon change, a Regional Resources and environmental information system was established; and RS, GIS and biogeochemistry model were all used to evaluate the soil organic carbon change.

生物多样性研究中，动物多样性研究部分以步甲作为指示生物展开，方法为陷阱法；植物多样性研究部分采用田间试验以及遥感影像配合下的样方记录法；对区域土壤有机碳变化的研究采用遥感、地理信息系统和生物地球化学循环模型模拟相结合的方法。

PSO is a population-based optimization algorithm, which mimics the social behavior of animals in a flock. It makes use of individual and group memory to update each particle position allowing global and local search optimization. The objective function considered was the total weight of the structure subjected to stresses, displacements and forces constraints. The effects of the parameters were investigated as well and such combination of tuning parameters promote a better global search behavior avoiding premature convergence while rapidly converging to the optimal solution. Results showed the effectiveness of the proposed method by comparing with ANSYS Design Optimization Tool (zero-order method). The PSO with the tuning parameters makes it an ideal method for offshore wind turbines foundations optimization tasks.(2) A reliability analysis method for pile foundation bearing axial loads based PSOThe performance function of pile foundation's axially bearing capacity sometimes is nonlinear and complex, on the basis of geometric meaning of structural reliability index, an optimum model with PSO for structural reliability analysis under arbitrary random variables was established, The PSO algorithm is very efficient to solve global optimization problemsIts use in structural reliability field presents not only the advantage of its facility of implementation, but also the possibility to obtain the design point and the failure probability with a good accuracy. In addition, PSO is a zero order algorithm, for no derivative is necessary for its implementation.

本文的研究针对桩式海上风机基础结构的特点，在国内外有关研究成果的基础上对海上风机基础结构优化设计理论和可靠度方法一些相关问题进行了较为深入的研究，具体做了以下几个方面的工作：(1)基于粒子群优化的桩式海上风机基础确定性优化设计方法桩式海上风机基础的优化设计是一个复杂的、非线性约束的优化问题，针对传统的基于梯度信息的优化方法在处理非线性问题中易陷入局部最优解的问题，本文将一种耦合惩罚函数的PSO算法引入到海上风机基础结构概念设计中，PSO算法是从群体动物聚集觅食这一活动中受到启发而发展的，该算法利用个体和群体的信息共享不断改进自身的位置从而进行局部和全局最优搜索，本文中以桩和三脚架连接段直径及壁厚为设计变量，以基础总重量作为优化的目标函数，在给定的约束条件下建立了三脚架基础优化数学模型，另外本文还研究了PSO参数变化对结果的影响，协调的参数组合可以避免陷入早熟收敛而能够快速的获得全局的最优解，通过与ANSYS优化模块的计算结果比较验证了该方法的有效性，本方法为海上风机基础的确定性设计提供了一条有效的途径。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

A 2. 5～5. 5 MHz transducer was used according to the acoustic depth of the heart examined. Group B was used x-ray fluoroscopy (a Philip mono-rotatable C arm X-ray equipment) as image guide with routine method reported by american college of cardiology cardiovascular technology assessment committee. TTE guiding CSC in site: The guide wire was inserted into left subclavicular vein and inferior vena cava and it was displayed clearly by TTE. The sheath could be displayed clearly too by TTE. CSC was put through the sheath into right atrium in prone position and was thoroughly showed by TTE via untypical parasternal four-chamber view.

本研究对60例心内电生理检查患者，应用TTE作为影像学引导的可行性和临床应用的方法学进行了探讨，并对5只犬TEE引导射频导管消融术时大头电极导管到位的可行性进行了动物实验研究，具体为：(1)专用冠状窦电极导管置放时TTE引导的可行研究；(2)普通电极导管置放时TTE引导的可行研究；(3)专用冠状窦电极导管和普通冠状窦电极导管置放时TTE的对比研究：(4)TTE引导His束电极导管和右室电极导管到位的临床应用研究；(5)TTE在心内电生理检查中的应用研究；(6)TEE引导射频导管消融术大头电极导管到位的实验研究。

Three infectious bronchitis virus isolates named as Q1, J2 and T3 were isolated from proventricular tissue taken from vaccinated chicken flocks in China The biological characteristics of the three isolates were observed.

中文题名传染性支气管炎致腺胃病变毒株变异的分子基础及我国和东南亚分离株的分子流行病学研究副题名外文题名 Molecular basis for variation of proventriculus pathogenic IBV isolates and molecular epidemiology of IBV isolates from China and southeast Asia 论文作者蒋贻海导师陈溥言教授学科专业预防兽医学研究领域\研究方向动物分子病毒学及免疫学学位级别博士学位授予单位南京农业大学学位授予日期2002 论文页码总数127页关键词鸡传染性支气管炎传染性支气管炎病毒腺胃毒株馆藏号BSLW /2003 /S855 /9 从我国接种过传染性支气管炎疫苗的幼鸡群的腺胃组织中分离得到三个传染性支气管炎毒株Q1、J2和T3，并对它们的部分生物学特性进行研究。

The coeve to the plasma study of monoamine neurotransmitter in normal person and schozophreniac on four mian solar term in four seasons; tostudy the amount and the component of middle molecule substances in plasma on four main solar term in four seasons in nor- mal person and schizophreniac; to estabisht the experimental animal model of sehijorhrenia and the improve the measurement index; to study monoamine neurotransmitter value of brain in normal rats and experimental schizophrenia rats on four main soler terms; to study the amount and component of MMS in plasma of normal rats and experi- mental schizophrenia rats on four main solar terms, and to make The morphological study of the brain tissue in experimental schizophre- nix rats and normal rats.

健康人、精神分裂症患者一年四季血浆单胺类神经递质含量的研究。2。健康人、精神分裂症患者一年四季血浆中分子总量及组分的研究。3。精神分裂症动物模型的建立及评分标准的改进。4。正常大鼠、精神分裂症模型大鼠一年四季脑组织单胺类神经递质含量的研究。5。正常大鼠、精神分裂症模型大鼠一年四季血浆中分子总量及组分的研究。6。精神分裂症模型大鼠、正常大鼠脑组织形态学研究。

In order to understand the expressed genes, construct the known gene expressed profile, study the tissue-specific genes and the common genes from pig and chicken; In order to do primary researches for comparative genomics, functional genomics, QTLs mapping, animal molecular breeding and so on, we have constructed 3 cDNA libraries of Xiang Pig muscle tissue, Silkie Fowl muscle tissue and liver tissue, sequenced a large scale ESTs and analyzed their bioinformation from the ESTs of Silkie Fowl muscle tissue and liver tissue in our study.

为全面了解肌肉组织和肝脏组织的基因表达类型，构建猪、鸡肌肉组织和肝脏组织的功能基因表达谱，研究肌肉组织和肝脏组织的特异表达基因及这两种组织的共同基因；为比较基因组学的研究、功能基因组学的研究、QTLs定位等的动物分子育种做前期基础科研工作，本研究构建了香猪肌肉组织、丝毛乌骨鸡肌肉组织和肝脏组织的3个cDNA文厍，并对丝毛乌骨鸡的肌肉组织和肝脏组织进行了大规模的ESTs测序及其生物信息学的初步研究。

In addition, there have no enough studies in the mechanism of spermatogenesis arrest induced by heat, and also there are many negative effects in the male contraceptive methods during these years like surgical ligation of vas deferens. All these factors have brings researchers to found a safety and efficacy methods for the problems in male reproduction.

因此，本研究通过高温处理未成年动物的模型，从细胞及分子水平探讨高温作用后生精功能障碍的机理，并对高温引起不育的机制进行深入研究；这些研究对阻止、延缓或减弱上述因素对人类生育能力的损害有重要意义，同时也为临床治疗一些男性不育、指导未成年男性生殖保健和探索男性避孕措施的研究提供理论基础。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新