研磨

The result indicated that after the yellow seedlings of wheat was triturated by the high speed tissue triturating machine, high purity mitochondria could be exacted by density gradient centrifugation, and then the mitochondria was ruptured by the 5% SDS and protease K, and the protein was wiped off by organic liquid, pure mtDNA could be extracted.

结果表明，经高速组织匀浆捣碎机研磨小麦黄花苗，简易的密度梯度离心技术快速抽提大黄的高纯度的线粒体，5% SDS和蛋白酶K裂解线粒体及有机溶剂抽提去除蛋白质可获得纯度较高的mtDNA。

In order to establish the simple extraction method of wheat mitochondrial DNA, this study investigated the effects of triturating material, exacting mitochondria and exacting and purifying mtDNA from yellow seedling of wheat, respectively.

为了建立小麦线粒体DNA快速提取体系，以小麦黄化苗为材料，分别研究了材料的研磨、线粗体的抽提和mtDNA的提取及纯化过程中众因素的影响。

The products were identified by IR,NMR,MS and elemental analysis. In our method,K.OH was used in place of NaOH to synthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate was purified on a silica gel column with chloroform/ethylacetate(4:l,v/v) as eluent and further purified by triturating with hexane/acetone(2:l,v/v).The luminescence produced by DMAE-NHS is a flash light with maximumemission at 0.4s and decay half-time of 0.9s. The luminescence intensity is 6.11x10 cps/mol,which is affected by the composition of trigger and surfactant.

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿/乙酸乙酯(4：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，第二次柱层析用己烷/丙酮(2：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷/丙酮(2：1，Ⅴ：Ⅴ)进行研磨，过滤，洗涤，除去溶于己烷/丙酮(2：1，Ⅴ：Ⅴ)的部分，得到所需要的产品。

In this paper, purification of growth hormone receptor from ovine liver and identification of its biochemical character have been done.Firstly, by homogenizing, triturating, high-speed centrifuging, hypervelocity centrifuging and sucrose density grade centrifuging cell membrane of ovine liver was separated and its electron microscope slice wasprotein was disL~olved with TritonX-100,amL-thebinding activity between cell membrane or membrane protein and ~I-hGH using RRAwas measured, perfect saturated curve wasgained. Thirdly, specific binding of GILR protein was measured ,by SDSPAGE, its molecular weight is about 1 19KD.

本研究采取新鲜羯羊肝脏，匀浆、研磨，经高速离心，超速离心和蔗糖密度梯度离心，分离肝脏质膜，得到肝脏质膜的电镜切片：其次，用去垢剂TritonX-100增溶膜蛋白，利用放射受体分析法，测定细胞膜及膜蛋白与~(125)I-hGH的结合活性，均得到理想的饱和曲线；再次，将两点结合试验法与凝胶层析法结合检测到特异结合物，然后，经SDS-PAGE电泳分析，纯化后的样品呈现一条带，与标准分子量比较，得出分子量为119KD。

Mechanical Power Vises are rigidly constructed to give industry a truly dependable tool for milling, grinding, drilling, shaping and production as well as many other types of usage.

稳固结构的机械增压虎钳给予业界一个值得信赖的工具来进行铣、研磨、钻孔等加工，产量就如同其他型号之功能一样。

The main influence factors include the reactants proportion, whetting time, and the decomposing temperature. Decomposing temperature is the most influential factor and heating time is the next.

影响固相反应的主要因素有反应物的配比、研磨时间、热分解温度、时间等，其中分解温度是影响最大的因素，其次是加热时间。

Used to produce alumina cement, abrasive materials, ceramic industry, as well as a variety of aluminum compounds.

6制造矾土水泥，研磨材料，陶瓷工业以及化学工业可制铝的各种化合物。

High quality ceramic mounted stone with handle, diamond plated products, emery abrasive cream, alumina fiber sharpening stone, spring rubber polishing mounted stone, precision spring rubber polishing mounted stone, precision wool polishing mounted stone, high qualiy precision polishing cream.

中国·浙江省台州长市一砂磨具厂 China Zhejiang Taizhou Yisha Grind Factory 8D50 高档陶瓷带柄砂轮磨头、金刚石电镀制品、金刚石研磨膏、氧化铝纤维油石、弹性海绵抛光磨头、弹性精密橡胶抛光磨头、高精羊毛抛光磨头、高精抛光膏。

After adjusting, if the oil or water leeks continually, the rubber bowl, the oil seal and the axle sleeve should be changed if there is friction and it doesn't go smoothly, it should be grinded to avoid the bearing and the pump base being destroyed

经过调整后，如果油或水韭菜不断，橡胶碗，油封和轴套应该改变（如果有摩擦和不顺利，应研磨，以避免轴承和泵基地被破坏

The main business cleaning table, the coal jig, the spiral chute, the flotation machine, the spiral sorting machine, the ball mill, the stirring chest, the feeding engine, for the ore machine, the shaker, the breaker, the sand pump, the magnetic ore separator, the water power sorting machine, the water power cyclone, hits the granulated substance machine, the laboratory sand pump, the laboratory table, the laboratory spiral chute, the laboratory flotation machine, the laboratory ball mill, the laboratory breaker, the laboratory magnetic ore separator, the laboratory adjusts the stuffchest, the grinder, products and so on vacuumfilter, in addition may make to order each kind of model according to the customer request the dressing mechanical device.

主营选矿摇床，跳汰机，螺旋溜槽，浮选机，螺旋分级机，球磨机，搅拌槽，给料机，给矿机，振动筛，破碎机，砂泵，磁选机，水力分级机，水力旋流器，打砂机，实验室砂泵，实验室摇床，实验室螺旋溜槽，实验室浮选机，实验室球磨机，实验室破碎机，实验室磁选机，实验室调浆桶，研磨机，真空过滤机等产品，另可根据客户要求定做各种型号的选矿机械设备。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！