研磨

Methods The antimicrobial peptide of M. domestica pupa were produced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography of AKTA purifier. Then the antimicrobial peptides with anti-Toxoplasma activity were selected by haemacytometry and MTT colorimetric method.

通过损伤加感染的方法诱导家蝇蛹大量表达抗菌肽，然后经过研磨、离心和AKTA purifier层析等步骤，将家蝇蛹抗菌肽进行分离纯化，采用血细胞计数法和MTT比色法筛选对弓形虫速殖子有抑制作用的抗菌肽。

Methods The antimicrobial peptides of housefly larvae were induced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography. Then the antimicrobial peptides with anti-Toxoplasma activity were sieved by methyl thiazolyl tetrazolium colorimetric method and haemacytometry. The DNA contents of T.gondii tachyzoites were detected by flow cytometry.

通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，分离纯化并筛选出抗弓形虫作用的抗菌肽，采用流式细胞术分析其对弓形虫速殖子DNA含量的影响。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

The motion state and grinding theory of milling medium in tube object of vibrating mill are analyzed in this paper.

分析了振动磨筒体内研磨介质的运动状态及粉磨机理，在此基础上，从能量观点出发，对振动磨整机的功率消耗进行了研究，并提出了计算振动磨机功率的理论公式。

Soldiers were marching outside the gate, blowing trumpets and beating tympani and drums.

於是，渔夫往回走，到家时一看，整座宫殿都由研磨抛光的大理石砌成，石膏浮雕和纯金装饰四处可见。

Soldiers were marching outside the gate, blowing trumpets beating tympani drums.

於是，渔夫往回走，到家时一看，整座宫殿都由研磨抛光的大理石砌成，石膏浮雕和纯金装饰四处可见。

For example, ultramarine, made by the grinding of lapis lazuli, and red pigments made of sulphur and silver , were admired for their cost

如由天青石研磨制成的佛青色、用硫和银制成的红颜料，因其高昂的费用而倍受青睐。

For example, ultramarine, made by the grinding of lapis lazuli, and red pigments made of sulphur and silver, were admi red for their cost

如由天青石研磨制成的佛青色、用硫和银制成的红颜料，因其高昂的费用而倍受青睐。

Continuous glass filaments as unclassifiable or probably non carcinogenic.

加强型的连续玻璃纤维丝产品。当切割、研磨、制造时通常不会产生吸入性颗粒。 IARC 组织认为连续纤维丝不会

He body undulating, grinding against me.

他身体起伏，对我研磨。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！