石油醚

The analysis methods for the determination of microor trace elements in high moisture jellyfish were developed. The fatty acid compositions indifderent parts of fresh jellyfish were determined by GC/MS method. Thirty-five fatty acids wereidentified, and most of them were found in R. esculentum jellyfish for the first time. Especially,two unusual very long chain polyunsaturated fatty acids that were never detected in the otherjellyfish also were determined. Amino acids were abundant in R. esculentum jellyfish, especiallycontaining sulfur amino acids, and could be supplied for human diet. The polysaccharide inumbrella part of jellyfish was composed of glucose, galactose and uronic acid, and its molecularweight was 40,000, but the polysaccharide of the oral arms part consisted of glucose, mannose andglycuronic acid, and its molecular weight was 43,000. Above-mentioned data were never reported.The ethanolic extract of oral arms part of jellyfish were extracted by different polar solvents(petroleum ether, acetic ether, n-butanol), and antibacterial activity was tested to these extracts byfour species of terricolous pathogenic bacilli and three species of botanic pathogenic fungi. Theresult demonstrated that the petroleum ether extract had certain bactericidal activity for twospecies of pathogenic bacilli, and n-butanol extract had certain inhibited activity on apple rotpathogenic fungus.

建立了高含水量的海蜇产品中微量、痕量元素成分测定的分析方法；采用 GC/MS 方法测定了新鲜海蜇不同部位的脂肪酸组成，共鉴定出 35 种脂肪酸，其中大多数脂肪酸是首次在海蜇中被检测到，尤其是两种不常见的 C24：5 超长链多不饱和脂肪酸的分析和鉴定在其它水母种属中也从未见报道；海蜇三个部位中氨基酸成分齐全，含量丰富，含硫氨基酸含量较高，可与其它食物蛋白质的氨基酸互补；其中海蜇皮多糖是由葡萄糖、半乳糖和糖醛酸组成，分子量为 40，000，海蜇头多糖是由葡萄糖、甘露糖和糖醛酸组成，分子量为 43，000，以上工作均未见报道；利用石油醚、乙酸乙酯、正丁醇三种不同极性溶剂分别萃取海蜇头乙醇浸提物，用纸碟法和生长速率法分别对四株陆源病原菌和三株植物病原真菌进行了抑菌实验，结果表明海蜇头石油醚提取物和正丁醇提取物具有一定的抑菌活性。

Primary extract the active elements from the root,stem and foliage of Artemisia annua L with petroleum ether (30~60℃), petroleum ether (60~90℃), ethanol, acetone and water were carried out in laboratory, then further separation of active elements was detected by column chromatography, and then the separated elements′acaricidal activitie was measured against Tetranychus cinnabarinus.

采用石油醚（30~60℃）、石油醚（60~90℃）、乙醇、丙酮和水等5种溶剂对采自6月份的黄花蒿的根、茎、叶分别进行活性成分的初步提取，然后用柱层析进行分离检查，并进一步用分离得到的组份对朱砂叶螨进行生物测定。

The exact of petroleum ether was the acaricidal active site of Eungenia caryophyllata. 3.Isolation, purification and identification of compounds with acaricidal activity in Eugenia Caryphyllata: The exact of petroleum ether was separated into 6 parts by silica gel column chromatography with gradually increasing polarity of petroleum ether: ethyl acetate. The part C was demonstrated to be the most effective part by acaricidal activity test in vitro,and the time for it to kill all experimental mites was about 20 min.

丁香杀螨活性成分的追踪分离、纯化与结构鉴定：采用硅胶柱层析法，以石油醚：乙酸乙酯溶剂系统梯度洗脱，对丁香石油醚提取物进行分离，得到A~F 等6 段混合物，离体杀螨试验发现，C 段混合物具有突出的杀螨活性，50%浓度平均15min可杀死全部供试螨虫。

The models of auricular edema in mice by dimethyl benzene and increased permeability of celiac blood capillary by acetic acid were studied,the results showed that the petroleum ether and acetic ester fraction of fermentation product of Calvatia gigantea could inhibit ear edema;acetic ester and n-Butanol fraction of fermentation product could reduce the permeability of celiac blood capillary by acetic acid.

大秃马勃Calvatia gigantea发酵液的石油醚、乙酸乙酯和正丁醇提取物进行二甲苯诱导小鼠耳廓急性炎症和醋酸所致小鼠腹腔毛细血管通透性实验，结果表明大秃马勃Calvatia gigantea发酵液石油醚提取物处理组、乙酸乙酯提取物处理组对二甲苯诱导小鼠耳肿胀具有明显的抑制作用；大秃马勃Calvatia gigantea发酵液乙酸乙酯提取物处理组、正丁醇提取物处理组对醋酸所致小鼠腹腔毛细血管通透性具有明显的抑制作用。

The free fatty acids were extracted with petroleum ether and were separated on silicon gel column chromatography. The ethyl acetate-soluble lipid fraction and water-soluble lipid fraction were subjected to hydrochloric acid-catalyzed methanolysis, then were extracted with petroleum ether, respectively.

用石油醚提取低极性组分，经硅胶柱层析分离得到游离脂肪酸；用乙酸乙酯提取酯溶性神经酰胺及其衍生脂类，提取物和残余的水溶性脂类分别经盐酸甲醇解，水解产物再用石油醚提取。

The result indicates that the best method for extracting β-carotene is to saponify the test specimen one hour with 4% NaOH methyl alcohol solution in 60-70'c and then to separate with 3:7 acetone-benzine component solvent as extraction solvent, MgO as the absorbent, the acetone-benzine as the eluent. Extracted solvent has been determined in the color dish condition (1cm) by ultraviolet spectrophotometer at 447 nm. The result shows that the β-carotene in carrotis 0.134 mgg^(-1), 0.0863mgg^(-1) in red pepper and 0.0426 mgg^(-1) in spinach.

结果表明，比较优先的β-胡萝卜素提取方法是：试样用4% NaOH甲醇溶液在60℃~70℃皂化1h，用3：7的丙酮－石油醚混合溶剂提取，以Mgo为吸附剂，丙酮－石油醚为洗脱剂分离，提取液用紫外分光光度计在波长447nm，1cm比色皿条件下测定，得出蔬菜中β-胡萝卜素含量：胡萝卜为0.134mgg^(-1)，红椒为0.0863mgg^(-1)，菠菜为0.426mgg^(-1)。

The result shows that: the extraction of petroleum ether, polysaccharide and sodium copper chlorophyllin from banana leaves perform higher spumescence ability and foam stability. The critical concentration form pastern namely CMC and the surface tension in this concentration are 1.0 g/l (34.295 mN/m), 1.0 g/l (42.398 mN/m), 3.0 g/l (40.123 mN/m) respectively, which the value is close or excess the reference samples. The emulsify capability of the extraction of petroleum ether is the best in these active ingredients, and close to the emulsify capability of Tween-80. The emulsify capability of polysaccharide and sodium copper chlorophyllin from banana leaves are follow then.

试验结果表明：香蕉叶石油醚抽出物、叶多糖和叶绿素铜钠盐具有良好的起泡性和稳泡性；其临界胶束浓度CMC值和其该浓度下的表面张力分别为：1.0g/l（34.295 mN/m），1.0g/l（42.398 mN/m），3.0g/l（40.123 mN/m），已经接近或超过参照物的表面活性；香蕉叶石油醚抽出物的乳化能力最强，乳化性能已接近乳化剂吐温-80，其次为叶多糖和叶绿素铜钠盐。

Five species of solvents, namely, acetone, ethyl acetate, ether, trichloromethane and petroleum ether were selected to obtain extracts from Portulaca oleracea L. with microwave assistance and the biological activities of each extract was tested against Plutella xylostella L.

在微波加热条件下，以丙酮、乙酸乙酯、乙醚、三氯甲烷和石油醚为溶剂对马齿苋干粉进行提取，试验了各溶剂提取物对小菜蛾幼虫的触杀、胃毒及拒食活性，确定了石油醚提取物触杀作用的LD50。

The allelopathic bioactivities on mycelial growth and the dose-activity relationships of two different Eucalyptus globulus leaves extracts,petroleum ether extract and chloroform extract,against five phytopathogens,Pyricularia oryzae,Rhizoctonia solani,Fusarium solani,Venturia pyrina,and Alternaria solani,were determined by mycelial growth rate method in PDA petri dish.

采用菌丝生长速率法，在PDA培养基上测定了蓝桉叶片的石油醚和氯仿提取物对水稻稻瘟病、小麦纹枯病、蚕豆根腐病、梨黑星病、番茄早疫病5种植物病原菌的化感作用，研究了蓝桉叶片的石油醚和氯仿提取物与病原菌的剂-效关系和提取物对病原菌菌丝生长速率的影响。

Result: The anticancer active component includes: taxol 9.45%、cephalomanine: 9.63%、10- deacetylbaccatinⅢ: 22.64%、total flavonoids: 19.15%. 3. The preparation of Seiadopitysin control Methods: Take dry Ginkgo leaves and extracted three times by sonication with 95% EtOH, solutions were concentrated under vacuum, after defatting by petroleum benzin, the sample was extracted with EtOAc and the solutions were concentrated under vacuum.

金松双黄酮对照品的制备方法：取干燥银杏叶以95％乙醇超声提取3次，提取液合并，减压浓缩，石油醚脱脂后，用乙酸乙酯萃取，萃取液减压浓缩，经硅胶柱(6cm×80cm)层析，石油醚-乙酸乙酯(9∶1～5∶5)梯度洗脱，产物用甲醇多次冲淋，再以二甲基甲酰氨溶解，反复重结晶，得黄色粉末结晶。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。