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Ointment extracted from Plastrum Testudinis was separated by silica gel column chromatography, gradiently eluted by petroleum ether-ethyl acetate, and 16 (Ts-1~Ts-16) components were obtained, which were biologically evaluated by MTT assay and flow cytometry on the proliferation of rat marrow-derived mesenchymal stem cells. Results showed that Ts-12 induced proliferation compared with the control group ( p <0.05), while Ts-4 showed inhibitive effect. Other groups showed no significance ( p >0.05). GC-MS was used to analyze the chemical components in different groups.

中药龟板提取物浸膏经硅胶柱层析,用石油醚-乙酸乙酯作洗脱剂,梯度洗脱,得到16个组分;采用MTT法和流式细胞技术研究了它们对鼠骨髓间充质干细胞的增殖作用,实验结果显示组分Ts-12具有促进rMSCs增殖的作用( p <0.05),而组分Ts-4则表现出抑制rMSCs增殖的作用( p <0.05),其它样品对rMSCs的增殖作用不显著( p >0.05),不具统计学意义。

objectiveto predict the promoting effect of chinese medicine on proliferation of rat bone marrow mesenchymal stem cells by bioactivity fingerprint in vitro.methodsthe ointment of plastrum testudinis was extracted by petroleum ether,ether and dichloromethane in burn to gain the extracts.the effects on the proliferation of bmmscs were examined by mtt assay and flow cytometry analysis, and each sample was studied by gc-ms.

目的运用生物活性指纹图谱预测其它中药能否促进骨髓间充质干细胞(bone marrow mesenchymal stem cells,bmmscs)。方法龟版浸膏依次用石油醚、乙醚、二氯甲烷提取,得到溶剂提取物,用mtt 比色法及流式细胞仪器研究了溶剂提取物干细胞活性,采用gc-ms 技术研究每一个提取物,根据总离子流色谱图的相同保留时间和相应峰面积,获得龟版浸膏 gc-ms 生物活性指纹图谱。

The antineoplastic effective parts of Plumbago zeylanica L.were chloroform and petroleum ether extracts.

白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好,值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

Objective To investigate the chemical constituents of the root of Solanum torvum Swartz.

目的 研究金钮扣根石油醚部位和醋酸乙酯部位的化学成分。

Properties: colorless needle-like crystals, melting point 105 ~ 106 ℃, optical rotation +51 °(c = 0.978, chloroform), other reports shows that by ethanol recrystallization the melting point 104 ~ 105 ℃, methanol re - the formation of solvate crystal melting point 84 ℃.it is Soluble in acetone, chloroform, insoluble in ether, petroleum ether, insoluble in water.

性质:无色针状结晶,熔点105~106℃,旋光度+51°( c=0.978,氯仿),还有报道经乙醇重结晶熔点104~105℃,经甲醇重结晶形成的溶剂化物熔点84℃。易溶于丙酮、氯仿,难溶于乙醚、石油醚,不溶于水。

Guagzhou Chenliji Pharmaceuticel Factry,Guangzhou,Guangdong 510180)Abstract To establish a method for the identification of storax and the determination of cassia acid in storax by HPLC. Methods: TLC and HPLC were applied.

HPLC在适当的条件下,采用色谱峰检出法,TLC采用硅胶GF254薄层板,ψ(石油醚∶正己烷∶甲酸乙酯∶甲酸)=10∶30∶15∶1为展开剂,荧光及硫酸乙醇喷雾两次检出;定量采用ODS C18色谱柱(4.6 mm×150 mm×5μm),流动相:ψ=58∶42∶0.3,检测波长271 nm,流速:1 mL/min。

The acaricidal activity of the extracts of n-butanol and distilled water were not detected in this test.

其中丁香石油醚提取物杀螨活性最为突出,是丁香的主要杀螨活性部位。

Then the extracts of petroleum ether,chloroform,ethyl acetate,n-butanol and distilled water were extracted by solvent extraction,and whose acaricidal activity were detected by killing Psorptes communis cuniculi in vitro.

石油醚、氯仿、乙酸乙酯、正丁醇、蒸馏水五种溶剂对丁香进行部位提取,采用离体杀螨试验观察各部位提取物的杀螨活性。

From Acropora pulchra and Porites Compressa Dana,a mixture of sterol has been isolated respectively.

从佳丽鹿角珊瑚乙醇提取液的乙酸乙酯部分和扁缩滨珊瑚乙醇提取液中的石油醚部分各分离出一甾醇混合物。

ResultThe Buxus Microphylla leaf ethyl acetate extraction (high-dose and low dose) have obvious effect on reducing the serum total cholesterol and triglyceride, moreover, the high-dose ethyl acetate extract reducing blood lipid was better than the other extracts.

结果 小叶黄杨叶乙酸乙酯提取物的高、低两个剂量组均有显著的降低血清总胆固醇和三酰甘油的作用,小叶黄杨叶的水提取物和石油醚提取物均有显著的降低血清中总胆固醇的作用,小叶黄杨叶氯仿提取物及正丁醇提取物均无降血脂的作用。

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