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The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因,并将该基因克隆至真核表达载体pcDNA3.1中,成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后,通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

Velocity measurements, both mean and instantaneous, are made using a two-component laser Doppler anemometer.

速度测量,都意味着和瞬时,所使用的是双组分激光多普勒测速仪。

At the same time, large angular acceleration will cause impact in the hydraulic system.

溢流缓冲主要是降低瞬时压力冲击。建立相应的功率状态方程。

We make use of a micro-cell network model and a planar heat source, based on the atomic layer thermopile model, to obtain a time-dependence formula of the effect.

我们通过建立微电源网络模型、瞬时平面热源模型并结合原子层热电堆模型,得到了随时间变化的激光感生电压公式。

The Earth's instantaneous axis of rotation movement in the earth's interior.

地球瞬时自转轴在地球内部的运动。

The simulation results show that the vector control system based on this improved scheme has better dynamic and static performance. It solves a lot of problems such as larger error in the MRAS based on the back electromotive force when the motor runs across the zero speed and the instability based on the instantaneous reactive power in the regenerating mode and has high estimating precision.

仿真表明基于该方法的矢量控制系统动静态性能好,能有效解决反电动势模型参考自适应方法在速度过零点辨识误差大以及瞬时无功功率模型参考自适应方法在转速给定为负阶跃时转速不稳定问题,转速估计精度高。

The principle and system structure of direct measurement method and indirect measurement methods ( including thrust wall pressure integral method and ballistic pendulum method) are introduced.

针对脉冲爆震发动机瞬时推力、平均推力测量问题,介绍了直接测量法和间接测量法(包括推力壁压力曲线积分法和抛物摆法)的测量原理及系统构成。

From the perspective of minimum transmission losses, an instantaneous reactive power theory for systems with any number of phases and with or without neutral line is developed. Under the new definition, each quantity has its own physical meaning, and the zero sequence current can be decomposed into active and reactive components. Furthermore, a generalized compensation method for zero sequence current is developed.

从输电损耗最小的角度出发,提出了任意相无中性线或有中性线系统的瞬时无功功率理论,在新的理论下,每个量都有清晰的物理意义,且能分解出零序电流的有功分量和无功分量,并给出了通用的零序电流补偿方法,揭示了瞬时无功功率理论与传统平均意义下的无功功率理论的联系及本质区别,从而为研究HVDC-VSC及各种FACTS装置的无功功率控制奠定了理论基础。

When the nonlinear layer which has weak nonlinearity is thin and far away from the seismic source which be low energy, compared with the corresponding results in linear antis, the medium nonlinearity produces abort 1/10~1/4 and 1/10~1/3 differences of instantaneous dominant frequency aid bandwidth. These instantaneous feature differences of seismic waves induced by the nonlinearity of media become weaker and weaker with the decrease of source energy. However, with the increase of the wave travel path within the mummer layer, the nonlinear accumulation effect could still keep such phenomena as the waveform distortion and instantaneous dominant frequency and bandwidth variations in records.

在本文给定的弱非线性介质中(非线性炽较薄且远离震源),在震源能量较低的情况下,介质的非线性效应会造成地震波瞬时主频和频宽相对于线性介质情况分别为1/10~1/14和1/10~1/3左右的变化量此外,随着入射波能量的下降,介质非线性所造成的地震波瞬时特征的变化量减小,但随着地震波在穿越非线性介质时路径的加长,非线性作用的累积效应使得波形畸变和频率分布特征的变化等现象得以维持。

A prediction model has been proposed to deal with threshold voltage shift as a function of 1MeV neutron flux and gate oxide thickness, and to deal with room-temperature annealing of threshold voltage shift induced-by 〓Coγ as a function of electric field and gate oxide thickness. The commonness and individuality of MOS device degradation between hot-carrier effect and ionizing radiation were investigated. The dependence of substrate current, gate current and threshold voltage shift due to hot-carrier on gate oxide thickness were simulated with MEDICI-2D simulator. The photocurrents of PN junction and bipolar transistor were studied. Their transient responses on varied bias voltages, pulse durations and absorbed doses were simulated. The influences of NPN base width on base and collector photocurrents were investigated. We also studied 1MeV neutron displacement damage in PN junction, and calculated reverse current leakage under the neutron flux of 2. 67×10〓 per square centimeter. 3 The study of nondestructive screening method for MOS radiation tolerance A theoretical and experimental study of nondestructive screening methods for radiation tolerance of nMOS and pMOS were firstly fulfilled. We determined the informative parameters pertinent to the method and proposed how to deal with experimental data and verify obtained results statistically, as well as make the required steps for nondestructive screening. Based on the experimental data of 180 samples of discrete pMOS devices, the relation between sample quantity and correlation coefficient, screening equation, and other significant results were obtained from the threshold voltage shifts of pre-and post-irradiation samples.

二、分立器件电离和位移辐射损伤响应研究在国内首次开展了电离辐射引起的氧化层阈值电压漂移和退火理论研究,编程计算了1MeV中子引起的MOS器件阈值电压漂移与中子通量及栅氧厚度的关系,计算了〓Coγ光子引起的阈值电压漂移及退火的时效曲线与纵向电场和栅氧厚度的关系等,对评估MOS型集成电路电离损伤和加固方法研究,以及论文中开展的MOSFETs无损筛选方法研究奠定了基础;分析了MOS器件热载流子损伤效应与辐射电离损伤效应之间的异同性,利用二维模拟软件MEDICI-2D模拟了衬底电流和栅电流,在不同栅氧厚度下随栅压的变化曲线,计算了热载流子效应造成的阈值电压漂移,研究结果对当前存在的以热载流子效应研究取代电离辐射损伤实验研究的设想,具有重要的参考价值;开展了PN结和双极晶体管电离光电流研究,计算了不同偏压、不同辐射脉宽和不同吸收剂量下PN结光电流的瞬时响应,计算了不同辐射脉宽下NPN晶体管基极电流和收集极电流的瞬时响应,以及基区宽度对NPN晶体管光电流的影响,研究结果对双极器件抗电离辐射损伤响应及加固方法研究具有重要意义;在国内首次开展了器件位移损伤的理论研究,分析了辐射产生的缺陷对电性能的影响,计算了注量为2.67×10〓cm〓的1MeV中子产生的缺陷对PN结反向电流的影响。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

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