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But androgen receptors were found to be nearly non-existent in the brains of newborn animals.

但是他们发现这些睾丸酮受体在动物幼体内的含量几乎为零。

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

They existed as dosage-effect and time-effect. All of these results proved that Sertoli cell was the first target cell, spermatogenic cell was the second one and mitochondrion was the target. Otherwise, we proved this compound can disturb the development and growth of sperms, influence the activity and amount of sperms and increase the deformation ratio.

结合组织病理和性激素及酶活性的改变,揭示该化合物睾丸毒性的靶细胞是支持细胞,其次是生精细胞,作用的靶点是细胞内的线粒体,此外还证明该化合物干扰精子的生长和发育,影响精子的活力和数目,增加畸形率,是雄性精子畸变的诱导剂。

No postive of SARS-Cov signal was detected, but there were extensive autoantibodies of IgG and IgA in the seminiferous epithelium.

原位杂交没有检测到睾丸内存在SARS病毒,但是在生精上皮内存在大量的自身抗体IgG和IgA。

Blood-Testis Barrier is the harrier between capillary vessel and seminiferous tubule which is important to produce sperm and maintain male procreation function.

血睾屏障是睾丸内毛细血管与精小管之间存在的屏障结构。血睾屏障对精子的正常发生及雄性生殖功能的维持具有重要的作用。

Concept of intraepithelial neoplasm and cervical carcinoma;etiology, pathogenesis, pathologic types, pathologic changes, pathway of metastasis, staging and prognosis of cervical carcinoma,endometrial carcinoma,breast carcinoma,and protatic carcinoma; pathogenesis and pathologic changes of endometrial hyperplasia, fibrocystic disease of breast, benign prostatic hyperplasia.

子宫颈上皮内肿瘤和子宫颈癌的概念,子宫颈癌,宫内膜癌,乳腺癌,前列腺癌的病因,发病机制,病理类型,病理变化,转移途径,分期和预后。卵巢和睾丸肿瘤的分类原则。子宫内膜增生症,乳腺纤维囊性变,良性前列腺增生的发病机制和病理变化。

Methods Samarium nitrate was administrated to mice at different doses (0,5,50,500,2000mg/L) for 90d.The contents of samarium and trace elements in testicle were observed.

110只雄性ICR分为5组,小鼠分别自由饮用0,5,50,500和2 000mg/L的硝酸钐水溶液90d,测定睾丸内钐和微量元素含量的变化。

Objective: The very nature of spermatogonial stem cellsis still poorly understood.

目的 :目前对人睾丸内精原干细胞的生物学本质特性尚缺乏深入研究。

under acute and chronic pain stress, reproduction of male rats was suppressed in virous extent.

疼痛应激能诱导睾丸内annexin 5在蛋白质和mRNA水平在不同时间点可能发生量的变化。

METHODS: Using tissue culture technique, the maximal non-toxic concentration of luteolin in swine testicle cell culture was measured, and the inhibitory effect of luteolin on TGEV was measured by cytophathogenic effect observation and crystal violet measurement. In addition, the degrees of inhibition of the luteolin on CPE of virus in cell culture were observed by changing sequence of adding the vires and the drag. RESULTS : The maximal non-toxic concentration of luteolin was 873.39 μmol/L.

采用细胞培养法,通过结晶紫摄入量测定结合细胞病变作用,观察木犀草素在不同的加药方式下,对感染TGEV的猪睾丸细胞的保护作用、结果:TGEV病毒先吸附ST细胞再加入药液,先与TGEV病毒孵育再接种于卯细胞,或药液先与细胞孵育再用病毒感染等不同方式下,木犀草素在0.84-54.50μmaol/L浓度范围内,均能明显地提高细胞的存活数。

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