着丝粒

Primary culture and subculture were adopted using lung cells of a female Elaphodus cephalophus' embryo. A new karyotype and a pair of big telocentric chromosomes were found. The diploid has 48 chromosomes, and the karyotype formula is 2M+2ST+42T+XX. C-banding analysis shows that these chromosomes are homologous with those reported relevant chromosomes. This article is the first to report the polymorphism of Elophodus cephalophus B-chromosomes and studies it's transmitted mechanism.

采用原代培养和传代培养法，对1头毛冠鹿的胚肺组织细胞进行观察，发现一种新的核型，其二倍体染色体数目为2n=48，核型公式为2M+2ST+42T+XX，出现1对大的末端着丝粒染色体，C-带显示该染色体与已报道的相关染色体同源，首次提出毛冠鹿B染色体多态，对其传递机制进行了探讨。

Software and results were saved in EXCEL for consequent statistic operation and obtaining chromosome patterns. 4 indexes were chose, such as chromosome karyotype, C-band pattern, chromosome behavior and B chromosome for comparative analyses, and adopted SPSS to reconstruct dendrogram. Combined with studies on insect morphology and insect geography, we made a detailed analysis and speculation on the relationship of taxa concerned. Finally we got the conclusions as follows: 1. The number of chromosomes in grasshoppers of Oedipodiae is 2n 〓=23=22+XO. All chromosomes are telocentric chromosomes. Most of the genome formulas are 3L+6M+2S+X and 3

论文选取染色体核型、C-带带型、染色体行为和B染色体这4个方面的指标进行比较分析，运用SPSS系统分析软件建立表达各属和各种之间亲缘关系的系统树，并结合昆虫形态学和昆虫地理学等方面的研究成果，对其亲缘关系进行了比较详细的分析和推测，得出以下结论：一、斑翅蝗科蝗虫的染色体数目都稳定在2n〓=23条，性别决定机制是XO型，染色体均为端部着丝粒，染色体组式以3L+6M+2S+X和3L+7M+1S+X这两种类型为主，即3对大型染色体，6～7对中型染色体和2～3对小型染色体，X染色体以中型为主，在染色体组中主要排列为4～7位。

The next phase is telophase , followed again by cytokinesis.

着丝粒最终分离，每个姊妹染色单体分向两极。

Using a 3H-labelled 7.3kb fragment of 18S and 28S rRNA g enes as a probe and by chromosomal in situ hybridization,it was demonstrated tha t the autoradiographic silver grains to be located along entire p+ part of the m arker chromosome.

应用3H标记的7.3kb长的rRNA基因探针进行染色体原位杂交，自显影银颗粒沿整个p+区域分布，p+上的银颗粒数是正常近端着丝粒染色体短臂上银颗粒平均数的5倍。

Lacryma-jobi and S. bicolor only relatively strong or strong CRM signals primarily located in the centromeric regions were detected.

在薏苡和高粱中仅测检到主要分布在着丝粒区的较强或强的CRM信号。

Lines which were 2n=21"+1" and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group.

对导入赖草优质耐盐基因的株系Line15进行了C-分带、荧光原位杂交、SSR相结合的鉴定，确认Line15是一个二体异附加系，附加的一对染色体与小麦的第二同源群长臂、第三同源群着丝粒和第七同源群短臂密切相关，并确定了Line15的外源染色体C-分带特征带型，为目后鉴定Line15附加系的真伪提供了简便快捷的途径；对抗旱附加系Line17进行了荧光原位杂交和SSR检测，得出Line17也是一个二体异附加系，外源染色体与小麦的第四同源群短臂密切相关。

It represents 99.4% of the euchromatic DNA and includes one megabase of heterochromatic sequence within the pericentromeric region of the short and long arm of the chromosome.

其中99.4％是常染色质，在着丝粒周围短臂和长臂区域还有1Mb的异染色质序列。

Site was detected near the centromere of metacentric chromosome, while the other homologous one had no hybridization signal.

在石蒜染色体上发现9个杂交位点，在1号长臂端部、5号近端部区域和6号长臂近着丝粒区域各有3个位点。

Karyotypic findings showed that all cultivars were mixoploid with chromosome number ranging from 49 to 57, and 54 was the most frequent number observed.

结果表明：（1）17个栽培小菊均为混倍体，染色体数在49～57之间变异，以54条为主；（2）染色体呈现多态性：多数品种由中部、近中部和近端部着丝粒染色体组成；最长与最短染色体的比值为1.62～2.39；臂比＞2的染色体所占的比率为11.11%～29.62%；核型不对称系数为59.74%～63.37%；3个地被菊品种和'奥运橙光'、'金陵白凤'（2n=53）核型类型为&2B&，其它品种为&2A&型。

These findings convincingly confirmed that the isochromosomes 8q were monocentric chromosomes and that they were truly isochromosomes 8q rather than isodicentric chromosome (8)(p11) or a translocation between a chromosome 8 and any other chromosomes.

由此证实此核型为47，XY，-8，+i(8)(q10)×2，而非等臂双着丝粒8q或8号染色体与其它染色体易位所致。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新