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眼镜蛇

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To study the effect and mechanism of fraction C isolated fromN Naja naja Actra Venomin inducing apoptosis of human leukemia cells.

目的:研究眼镜蛇蛇毒组分C诱导白血病细胞凋亡的作用及机制。

Objective:To investigate the effect of Naja Naja Actra Venom on leukemia cells.

目的:探讨中华眼镜蛇毒(Naja Naja Actra Venom,NNAV)抗白血病作用。

Naja naja atra venom could increase the expressions of AchE positive neurons in spinal cord of rat.

本实验观察大鼠眼镜蛇毒中毒后,随着时间的延长,腰髓前角AchE表达的变化,为临

ABSTRACT: Objective To isolate muscarinic peptide from Naja atra venom and to study the effect of MP in muscarinic cholinergic receptor.

目的 从舟山眼镜蛇Naja atra蛇毒中分离毒蕈碱样多肽,探讨其与M胆碱受体的关系。

In the second part of work, a basic peptide with mass weight of 7597 Da was isolated and purified from the Naja naja atra venom .

论文的另一部分工作通过对眼镜蛇毒进行分离纯化,获得了一个&极性很强&的组分,通过蛋白测序和分子量测定,组分确定为一个弱神经毒素,该毒素二级结构稳定,神经毒性很低。

METHODS CTX-d was fractionated and purified from Naja naja atra venom by column chromatography and RP-HPLC.

方法应用柱层析及RP—HPLC,从眼镜蛇毒粗毒中分离纯化细胞素素。

Three new cDNAs named NL1, NL2 and NL3 were cloned from the total RNA of Naja naja atra by RT PCR.

通过RTPCR 的方法,从广西产中华眼镜蛇总RNA 中克隆到3 个新的神经毒素cDNA序列,命名为NL1 、NL2 和NL3 。

Results The IgY solution obtained from water dilution method has specific binding with Naja atra and the titration increased coinciding with purity.

结果鸡卵黄经水溶法的粗提物与中华眼镜蛇毒即有较明显沉淀反应,其效价随着纯度的提高而增强。

The method of suceessive cation-exchange and RP-HPLC can efficiently purify CTX without PLA2 from Naja naja atra venom.

结论结合阳离子交换层析和RP—HPLC可从眼镜蛇毒中高效分离获得不舍PLA2的CTX。

To observe the growth inhibitory effects of 10 peptides (KD Ⅰ-1, KD Ⅰ-1 H, KD Ⅰ-2, KD Q Ⅰ-2, KD Ⅱ, KD Ⅱ-2, KD Ⅱ-3, KD Ⅲ-1, KD Ⅲ-2, KD Ⅲ-2) purified from KD, one of components in Naja Naja Actra venom, on human glioma U251 cell line.

目的:探讨中华眼镜蛇毒卡迪中进一步分离纯化的10个组分KDⅠ-1、KDⅠ-1 H、KDⅠ-2、KDQⅠ-2、KDⅡ、KDⅡ-2、KDⅡ-3,KDⅢ-1,KDⅢ-2和KDⅢ-2′对神经胶质瘤细胞U251的抑制作用。

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