真菌

Results The results showed that the average concentration of the bacteria, fungi and total microbes in the outdoor environment were 5.04×10^4, 3.75×10^3 and 5.41×10^4 cfu/m^3 respectively and the fungi accounted for 6.92% of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%, 36.0%, 4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes.

结果 合肥城区空气细菌总数均值为5.04×10^4cfu/立方公尺，真菌总数均值为3.75×10^3cfu/立方公尺，合计为5.41×10^4 cfu/立方公尺，其中真菌占6.92%；对不同采样点的空气细菌和真菌进行了初步鉴定，优势细菌属为微球菌属、芽孢杆菌属、假单胞菌属和葡萄球菌属，分别占总细菌的37.3%，21.0%，10.3%，9.5%；真菌共检出5属，其中优势菌属为曲霉属、青霉属、毛霉属、根霉属，分别占总真菌的42.0%，36.0 %，4.4%，4.2%。

In this research, swainsonine contents of three Oxytropis species were determined by the high performance liquid chromatography coupled to mass spectrometry. The population genetic diversity was also studied and the swainsonine related molecular markers were screened by ISSR. In vitro fungi cultures from the individual plant of O. glabra were performed and microbiological morphology of the colony and the conidiophore were investigated. The 5.8S rDNA/ITS sequences of fungi amplified from total DNA in both plants and fungal endophytes were studied. These sequences were analysed and compared, and they were also compared to the 5.8S rDNA/ITS sequence of fungal enduphyte Embellisia sp. L12 from the O.lambertii, O.sericea and A.mollisimus distributed in USA.

本研究采用高效液相色谱-质谱联用法测定了三种棘豆植物的苦马豆素含量；通过ISSR分子标记技术对三种棘豆种群遗传多样性及其苦马豆素相关标记进行了研究；将小花棘豆植物单株进行体外真菌培养，研究了菌落和分生孢子的微生物形态学特性，从植物总DNA和相同植株培养的真菌总DNA扩增真菌的5.8SrDNA/ITS区，进行了DNA序列分析与排序；联系单株小花棘豆的苦马豆素含量测定和内生真菌ITS序列分析结果，与从美国分布的二种棘豆(O.lambertii、O.sericea)和一种黄芪(A.mollisimus)中分离的内生真菌埃里砖格孢属Embellisia sp.L12株得到的结果进行了比较。

However, the area is less studied than other areas of endophyteresearch world wide. Therefore, a series of experiments were carried out in laboratory,glasshouse and field to compare the responses of endophyte infected plants andendophyte free plants of perennial ryegrass varieties to 10 fungal pathogensisolated from turf type ryegrass in Lanzhou. The pathogens used in the experimentswere Alternaria alternata、Bipolaris sorokiniana、Fusarium avenaceum、Curvularialunata、Ascochyta leptospora、Fusarium acuminatum、Fusarium chlamydosporum、Fusarium oxysporum、Fusarium solani and Gliocladium roseum. In addition, someprotective enzymes activity and alkaloids contents of E+ and E- plants were measuredand LpsA gene of the common strain - Lp19 and mutant strain -AR1 of Neotyphodiumlolii were sequenced to identify their ...

本研究在初步明确兰州市坪用黑麦草内生真菌及其病原真菌多样性的基础上，在实验室、温室和田间开展了系列研究，比较了带内生真菌和不带内生真菌的黑麦草在离体和整株条件下对细交链孢、离蠕孢、燕麦镰孢、新月弯孢、小孢壳二孢、锐顶镰孢、厚垣镰孢、尖镰孢、腐皮镰孢和粉红粘帚霉等10种病原真菌的抗病性，测定了E+和E-植株数种保护酶活性及其生物碱含量的变化。

We detected the result with the chi square test and concluded that it has statistical significance (P.05). Conclusion The detection of 1-3-β-D glucosan in patients blood samples is a both effective and quick way to diagnose the fungal rhinosinusitis. The positive rate of the way is about 90%.

本研究共计对20例真菌性鼻窦炎(19例为真菌球型，1例为慢性侵袭性真菌性鼻窦炎)患者及5例对照组患者血浆中1-3-β-D葡聚糖进行检测，发现其中18例真菌性鼻窦炎患者血浆中1-3-β-D葡聚糖的含量大于试剂盒的诊断标准10pg/ml，实验的阳性率为90%，血浆1-3-β-D葡聚糖水平可以用于诊断真菌性鼻窦炎。

Result 1-3-β-D glucosan was found exceeding the diagnostic criteria (10pg/ml) of the kit in 18 cases of fungal rhinosinusitis; and it was found no more than 10pg/ml in all 5 cases of purely nasal septum deviation patients. One of the 18 cases of fungal rhinosinusitis patients is chronic invasive fungal rhinosinusitis, and its level of 1-3-β-D glucosan reaches to 21.82 pg/ml. We compared the result of the study group to the control groups, and found that there is significant deviation beteen the two groups.

结果 经过实验检测20例真菌性鼻窦炎(19例为真菌球型，1例为慢性侵袭性真菌性鼻窦炎)及5例对照组患者血浆中1-3-β-D葡聚糖的含量，可以发现其中18例真菌性鼻窦炎患者血浆中1-3-β-D葡聚糖的含量大于试剂盒的诊断标准10pg/ml，其中一例慢性侵袭性真菌性鼻窦炎患者血浆中1-3-β-D葡聚糖的含量达到21.82pg/ml，2例真菌性鼻窦炎患者血浆中1-3-β-D葡聚糖的含量小于10pg/ml，而对照组患者血浆中1-3-β-D葡聚糖的含量均小于10pg/ml，病例组与对照组间采用卡方检验测得卡方值为16.071，(P＜0.05)两组间有差异，有统计学意义。

The aims of the Cascade Mycological Society are: study fungi; educate members and the public about fungi identification and ecology; promote conservation of fungi; promote health and safety in the gathering and consumption of edible fungi; have fun!

这是客斯客特真菌学会的网站。该学会的目的是：真菌研究；教育其成员和公众对真菌的鉴别及其生态学；促进真菌的保护；提高可食性真菌的采集和消费的安全性；娱乐！

But when we examined the structure of cultivar of D. huoshanense, endomycorhiza was founded unexpectedly. And in the wild type of D. huoshanense, fungi was also founded in its stems and leaves. So the author thought that endomycorhiza is not only located in the roots of Dendiobium plants, but also exists in other organs of plants, including stems, leaves. As plant produces seeds, mycorhiza could enter into the cells of seeds. When mature seeds germinate in media, the endomycorhiza enter into the roots of cultivar.

石斛属植物与真菌共生，根为菌根，作者在研究霍山石斛组织培养型结构时发现，组织培养苗的根部也分布有真菌，而且在野生霍山石斛的茎、叶中发现有真菌，作者因而指出，真菌并不是完全分布在根部，而是遍布于整个植物体，推测植物形成种子时，真菌进入种子，所以在组织培养时，真菌随着种子的萌发，进入植物体根部。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

objective in order to investigate the variety of the airborne fungi in jingzhou area and study the relationships between airborne fungi and allergic diseases.methods we surveyed the fungi sensitizing of air in jingzhou city from feb.1th,2007 to jau.31th,2008 to quantitatively observe the daily airborne fungi and count under microscope through one year.results the fungi spores were present through all year.more than 20 taxa of airborne fungi have been collected,among which the most common fungi were uredinales,ustilaginales,alternaria,etc.fungi spores were ralated with the season and weather,the most aboudant period in sring and summer.conclusion the airborne fungi were seen one year and were mostly allergenic.many of then had seasonal distribution.

目的：调查荆州市气传真菌的种类、数量变化及飘散规律，了解优势菌群与过敏性疾病的关系，为荆州市空气真菌过敏症的防治提供参考。方法：2007年2月1日至2008年1月31日，在荆州市设曝片点，每日换片，收集全年气传真菌样品，在显微镜下统计，定量获得每天及全年的真菌数量。结果：全年均可见真菌孢子，共收集真菌种类20多种，其中最常见的有链格孢、锈菌、黑粉菌等，其数量和季节、气候有关，以春季、夏季最多，秋季和冬季依次降低。结论：气传真菌全年均有分布并具有较强的致敏性，部分真菌具有明显的季节性变化。

Ninety seven spores or sporocarps of VAMF were wet sieved from the rhizosphere soil of mycorrhized pteridophytes, from which 25 VAMF species which belonged to the genera of Acaulospora, Gigaspora, Glomus, Sclerocystis and Scutellospora respectively were identified, and the taxonomic characters of the 25 species were described. The Glomus fungi were the dominant VAMF in the rhizosphere soil of pteridophytes, they took up 44% in the identified 25 VAMF (11 species belong to Glomus); the fungi in Acaulospora and Sclerocystis in the next place, they took up 24% and 16% respectively in the identified VAMF; the fungi in the other genera took up 16%. In terms of the relationships of pteridophytes and the VAMF isolated from their rhizosphere soil, there was no specifity between the VAMF and their host.

从具有VA菌根的蕨类植物对应的根际土壤中用湿筛法筛取了97份VA菌根真菌的孢子或孢子果，从中鉴定出了分属于无醒囊霉属、巨孢囊霉属、球囊霉属、硬囊霉属和盾孢囊霉属的VA菌根真菌25种，并对这25种真菌的分类学特征进行了描述；球囊霉属真菌是蕨类植物根际土壤中的优势类群，已鉴定的25种真菌中，有11种属球囊霉属真菌，占44%，其次是无梗囊霉属和硬囊霉属真菌，分别占24%和16%，其他属的VA菌根真菌占16%，从分离鉴定的蕨类植物根际土壤中的VA菌根真菌与蕨类植物的关系看，VA菌根真菌与蕨类植物之间无明显的专一性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！